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Influenza Res Treat. 2010;2010:976084. doi: 10.1155/2010/976084. Epub 2010 Dec 14.
Shedding of the Pandemic Swine-Origin Influenza A Virus (H1N1) after Oseltamivir Administration.
Weng L, Wang Q, Wang W, Ren P, Deubel V, Toyoda T.
Source
Unit of Viral Genome Regulation, Institut Pasteur of Shanghai, The Key Laboratory of Virology and Immunology, Chinese Academy of Sciences, 411 Hefei Road, Shanghai 200025, China.
Abstract
We analyzed the virus shedding of an oseltamivir-treated patient who had been infected with the pandemic swine-origin influenza A (H1N1) virus which had an oseltamivir-sensitive neuraminidase. The virus was isolated from the pharyngeal swabs of the patient using MDCK cells, and the virus genome RNA was detected in the same samples both by real-time RT-PCR and RT-PCR. The virus was isolated until 44 h after oseltamivir administration although the virus genome was detected until one day after oseltamivir treatment was stopped. Due to their high sensitivity, RT-PCR and real-time RT-PCR may cause misdiagnosis by detection of viral genome which does not infect, and classical virus isolation and clinical symptoms are recommended for the evaluation of oseltamivir treatment.
PMID:
23074651
[PubMed]
Shedding of the Pandemic Swine-Origin Influenza A Virus (H1N1) after Oseltamivir Administration.
Weng L, Wang Q, Wang W, Ren P, Deubel V, Toyoda T.
Source
Unit of Viral Genome Regulation, Institut Pasteur of Shanghai, The Key Laboratory of Virology and Immunology, Chinese Academy of Sciences, 411 Hefei Road, Shanghai 200025, China.
Abstract
We analyzed the virus shedding of an oseltamivir-treated patient who had been infected with the pandemic swine-origin influenza A (H1N1) virus which had an oseltamivir-sensitive neuraminidase. The virus was isolated from the pharyngeal swabs of the patient using MDCK cells, and the virus genome RNA was detected in the same samples both by real-time RT-PCR and RT-PCR. The virus was isolated until 44 h after oseltamivir administration although the virus genome was detected until one day after oseltamivir treatment was stopped. Due to their high sensitivity, RT-PCR and real-time RT-PCR may cause misdiagnosis by detection of viral genome which does not infect, and classical virus isolation and clinical symptoms are recommended for the evaluation of oseltamivir treatment.
PMID:
23074651
[PubMed]
