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Realtime RT-PCR Assay Unable to Detect H7 Subtype Avian Influenza Viruses Isolated from Wild Birds
<nobr>Zheng Xing<sup>*</sup>,</nobr> <nobr>Carol Cardona,</nobr> <nobr>Phuong Dao,</nobr> <nobr>Beate Crossley,</nobr> <nobr>Sharon Hietala,</nobr> and <nobr>Walter Boyce</nobr> Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616; Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616; Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616; California Animal Health and Food Safety Laboratory; Wildlife Health Center
<sup>*</sup> To whom correspondence should be addressed. Email: zxing@ucdavis.edu<script type="text/javascript"><!-- var u = "zxing", d = "ucdavis.edu"; document.getElementById("em0").innerHTML = '<a href="mailto:' + u + '@' + d + '">' + u + '@' + d + '<\/a>'//--></script>.
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</td> <th align="left" valign="middle" width="95%"> Abstract</th></tr></tbody></table>
We report a failure of the current real-time RT-PCR H7 subtyping<sup> </sup>protocol, used in national avian influenza surveillance programs.<sup> </sup>Significant substitutions in primer and probe target sequences<sup> </sup>were identified, especially in wild bird viruses. The protocol,<sup> </sup>designed originally for detecting H7 influenza viruses in poultry,<sup> </sup>is not reliable for wild bird surveillance.
<nobr>Zheng Xing<sup>*</sup>,</nobr> <nobr>Carol Cardona,</nobr> <nobr>Phuong Dao,</nobr> <nobr>Beate Crossley,</nobr> <nobr>Sharon Hietala,</nobr> and <nobr>Walter Boyce</nobr> Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616; Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616; Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616; California Animal Health and Food Safety Laboratory; Wildlife Health Center
<sup>*</sup> To whom correspondence should be addressed. Email: zxing@ucdavis.edu<script type="text/javascript"><!-- var u = "zxing", d = "ucdavis.edu"; document.getElementById("em0").innerHTML = '<a href="mailto:' + u + '@' + d + '">' + u + '@' + d + '<\/a>'//--></script>.
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<table bgcolor="#e1e1e1" cellpadding="0" cellspacing="0" width="100%"> <tbody><tr><td align="left" bgcolor="#ffffff" valign="middle" width="5%">
</td> <th align="left" valign="middle" width="95%"> Abstract</th></tr></tbody></table> We report a failure of the current real-time RT-PCR H7 subtyping<sup> </sup>protocol, used in national avian influenza surveillance programs.<sup> </sup>Significant substitutions in primer and probe target sequences<sup> </sup>were identified, especially in wild bird viruses. The protocol,<sup> </sup>designed originally for detecting H7 influenza viruses in poultry,<sup> </sup>is not reliable for wild bird surveillance.
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