Check out the FAQ,Terms of Service & Disclaimers by clicking the
link. Please register
to be able to post. By viewing this site you are agreeing to our Terms of Service and Acknowledge our Disclaimers.
FluTrackers.com Inc. does not provide medical advice. Information on this web site is collected from various internet resources, and the FluTrackers board of directors makes no warranty to the safety, efficacy, correctness or completeness of the information posted on this site by any author or poster.
The information collated here is for instructional and/or discussion purposes only and is NOT intended to diagnose or treat any disease, illness, or other medical condition. Every individual reader or poster should seek advice from their personal physician/healthcare practitioner before considering or using any interventions that are discussed on this website.
By continuing to access this website you agree to consult your personal physican before using any interventions posted on this website, and you agree to hold harmless FluTrackers.com Inc., the board of directors, the members, and all authors and posters for any effects from use of any medication, supplement, vitamin or other substance, device, intervention, etc. mentioned in posts on this website, or other internet venues referenced in posts on this website.
We are not asking for any donations. Do not donate to any entity who says they are raising funds for us.
Announcement
Collapse
No announcement yet.
Specific antibody response of mice after immunization with COS-7 ...
what are COS-7 ?
Is this a new method to produce vaccine not in eggs ?
COS-7 cell derived avian influenza virus (H5N1) recombinant proteins
______
Original research Specific antibody response of mice after immunization with COS-7 cell derived avian influenza virus (H5N1) recombinant proteins
Journal of Immune Based Therapies and Vaccines 2007, 5:10doi:10.1186/1476-8518-5-10
<TABLE cellSpacing=0 cellPadding=0><TBODY><TR><TD><TR><TD>Published:</TD><TD>3 October 2007</TD></TR></TD></TR></TBODY></TABLE> Abstract (provisional)
To develop avian influenza H5N1 recombinant protein, the hemagglutinin (HA), neuraminidase (NA), matrix (M), and non-structural (NS1) of avian influenza H5N1 isolates from Thailand were engineered to be expressed in prokaryotic (E. coli) and mammalian cell (COS-7) system. The plasmid pBAD-His and pSec-His were used as vectors for these inserted genes. Mice immunized with purified recombinant proteins at concentration 50-250 ug intramuscularly with Alum adjuvant at week 0, week 2, and week 3 showed a good immunogenicity measured by ELISA and neutralization assay. The HA and NS recombinant proteins produced in COS-7 cells can induce specific antibody titer detected by neutralization assay significantly higher than corresponding recombinant proteins produced in E.coli system. The antibody produced in immunized mice could neutralize heterologous avian influenza virus determined by micro-neutralization assay. This study shows that avian influenza virus H5N1 recombinant proteins produced in mammalian cell system were able to induce neutralizing antibody response.
... engineered to be expressed in prokaryotic (E. coli) and mammalian cell (COS-7) system ...The HA and NS recombinant proteins produced in COS-7 cells can induce specific antibody titer detected by neutralization assay significantly higher than corresponding recombinant proteins produced in E.coli system.
From full text PDF: To construct the mammalian expression vector, ..., and NS1 protein expression in COS-7 cell line, ... DNA was used for transformation into ... E.Coli cells and ..., ... DNA to transform COS-7 cell line by using polyfect transfection system (Qiagen, USA) - from page 6 PDF full text; ... Imunization of mice - page 7,8 were immunized three times intramuscularly ... with ... and rM protein produced in E.coli and COS-7 cells plus an emulsion prepared with Alum adjuvant. ... One week after the last boosting, mice were sacrificed and whole blood was collected for imm. analysis. Than, ..., and rM protein produced in E.coli and COS-7 cells were selectedto immunize 5 groups of mice for each protein ...
Re: Specific antibody response of mice after immunization with COS-7 ...
This is interesting because not only are there the mass production aspects of using cell culture but they also refer to the fact that mammalian cell culture may be better than other types of cell culture in that the antigenic epitopes are modified similarly to that in naturally viral infected human cells. (This could lead to a more immunogenic and protective vaccine) ( http://pecf.epfl.ch/webdav/site/pecf...urebiotech.pdf Wurm, FM Nat Biotechnol 2004)
Tweet
Comment