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Denmark Tamiflu Resistant Sequence Released
Denmark has released the full sequence of isolate with H274Y.
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Re: Denmark Tamiflu Resistant Sequence Released
LOCUS CY043352 1424 bp cRNA linear VRL 04-AUG-2009
DEFINITION Influenza A virus (A/Denmark/528/2009(H1N1)) segment 6 sequence.
ACCESSION CY043352
VERSION CY043352.1 GI:255103492
DBLINK Project:37813
KEYWORDS .
SOURCE Influenza A virus (A/Denmark/528/2009(H1N1))
ORGANISM Influenza A virus (A/Denmark/528/2009(H1N1))
Viruses; ssRNA negative-strand viruses; Orthomyxoviridae;
Influenzavirus A.
REFERENCE 1 (bases 1 to 1424)
AUTHORS Gregory,V., Nielson,L., Zheng,X., Whittaker,L., Daniels,R. and
Hay,A.
TITLE Pandemic influenza A(H1N1) virus sequences
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1424)
AUTHORS Gregory,V., Nielson,L., Zheng,X., Whittaker,L., Daniels,R. and
Hay,A.
TITLE Direct Submission
JOURNAL Submitted (04-AUG-2009) WHO Collaborating Centre for Reference and
Research on Influenza, National Institute for Medical Research, The
Ridgeway, Mill Hill, London NW7 1AA, UK
FEATURES Location/Qualifiers
source 1..1424
/organism="Influenza A virus (A/Denmark/528/2009(H1N1))"
/mol_type="viral cRNA"
/strain="A/Denmark/528/2009(H1N1)"
/serotype="H1N1"
/isolation_source="gender:Female; age:21y"
/host="human"
/db_xref="taxon:664181"
/segment="6"
/lab_host="swine kidney 2, MDCK 3 passage(s)"
/country="Denmark"
/collection_date="09-Jun-2009"
/note="Oseltamivir resistant, Zanamivir sensitive,
Amantidine resistant (31N in M2), lineage: swl"
gene 1..1410
/gene="NA"
CDS 1..1410
/gene="NA"
/codon_start=1
/product="neuraminidase"
/protein_id="ACU00954.1"
/db_xref="GI:255103493"
/translation="MNPNQKIITIGSVCMTIGMANLILQIGNIISIWISHS IQLGNQN
QIETCNQSVITYENNTWVNQTYVNISNTNFAAGQSVVSVKLAGNSSLCPV SGWAIYSK
DNSIRIGSKGDVFVIREPFISCSPLECRTFFLTQGALLNDKHSNGTIKDR SPYRTLMS
CPIGEVPSPYNSRFESVAWSASACHDGINWLTIGISGPDNGAVAVLKYNG IITDTIKS
WRNNILRTQESECACVNGSCFTVMTDGPSDGQASYKIFRIEKGKIVKSVE MNAPNYYY
EECSCYPDSSEITCVCRDNWHGSNRPWVSFNQNLEYQIGYICSGIFGDNP RPNDKTGS
CGPVSSNGANGVKGFSFKYGNGVWIGRTKSISSRNGFEMIWDPNGWTGTD NNFSIKQD
IVGINEWSGYSGSFVQHPELTGLDCIRPCFWVELIRGRPKENTIWTSGSS ISFCGVNS
DTVGWSWPDGAELPFTIDK"
ORIGIN
1 atgaatccaa accaaaagat aataaccatt ggttcggtct gtatgacaat tggaatggct
61 aacttaatat tacaaattgg aaacataatc tcaatatgga ttagccactc aattcaactt
121 gggaatcaaa atcagattga aacatgcaat caaagcgtca ttacttatga aaacaacact
181 tgggtaaatc agacatatgt taacatcagc aacaccaact ttgctgctgg acagtcagtg
241 gtttccgtga aattagcggg caattcctct ctctgccctg ttagtggatg ggctatatac
301 agtaaagaca acagtataag aatcggttcc aagggggatg tgtttgtcat aagggaacca
361 ttcatatcat gctccccctt ggaatgcaga accttcttct tgactcaagg ggccttgcta
421 aatgacaaac attccaatgg aaccattaaa gacaggagcc catatcgaac cctaatgagc
481 tgtcctattg gtgaagttcc ctctccatac aactcaagat ttgagtcagt cgcttggtca
541 gcaagtgctt gtcatgatgg catcaattgg ctaacaattg gaatttctgg cccagacaat
601 ggggcagtgg ctgtgttaaa gtacaacggc ataataacag acactatcaa gagttggaga
661 aacaatatat tgagaacaca agagtctgaa tgtgcatgtg taaatggttc ttgctttact
721 gtaatgaccg atggaccaag tgatggacag gcctcataca agatcttcag aatagaaaag
781 ggaaagatag tcaaatcagt cgaaatgaat gcccctaatt attactatga ggaatgctcc
841 tgttatcctg attctagtga aatcacatgt gtgtgcaggg ataactggca tggctcgaat
901 cgaccgtggg tgtctttcaa ccagaatctg gaatatcaga taggatacat atgcagtggg
961 attttcggag acaatccacg ccctaatgat aagacaggca gttgtggtcc agtatcgtct
1021 aatggagcaa atggagtaaa aggattttca ttcaaatacg gcaatggtgt ttggataggg
1081 agaactaaaa gcattagttc aagaaacggt tttgagatga tttgggatcc gaacggatgg
1141 actgggacag acaataactt ctcaataaag caagatatcg taggaataaa tgagtggtca
1201 ggatatagcg ggagttttgt tcagcatcca gaactaacag ggctggattg tataagacct
1261 tgcttctggg ttgaactaat cagagggcga cccaaagaga acacaatctg gactagcggg
1321 agcagcatat ccttttgtgg tgtaaacagt gacactgtgg gttggtcttg gccagacggt
1381 gctgagttgc catttaccat tgacaagtaa tttgttcaaa aaac -
Re: Denmark Tamiflu Resistant Sequence Released
H274Y travel log
gb|GQ397279.1| Influenza A virus (A/Yamaguchi/22/2009(H1N1)) ... 36.2 0.73
gb|GQ365445.1| Influenza A virus (A/Osaka/180/2009(H1N1)) seg... 36.2 0.73
gb|GQ351316.1| Influenza A virus (A/Hong Kong/2369/2009(H1N1)... 36.2 0.73
gb|EU567011.1| Influenza A virus (A/Indiana/01/2008(H1N1)) se... 36.2 0.73
gb|DQ493078.1| Influenza A virus (A/Vietnam/CL2009/2005(H5N1)... 36.2 0.73
gb|DQ250165.1| Influenza A virus (A/Vietnam/CL2009/2005(H5N1)... 36.2 0.73
Denmark is not listed because sequence is not yet in searchable database.Comment
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Re: Denmark Tamiflu Resistant Sequence Released
HA travel log
gb|CY040549.1| Influenza A virus (A/swine/North Carolina/4395... 32.2 11
gb|CY039991.1| Influenza A Virus (A/New Jersey/8/1976(H1N1)) ... 32.2 11
gb|FJ688266.1| Influenza A virus (A/swine/Thailand/HF6/2005(H... 32.2 11
dbj|AB434328.1| Influenza A virus (A/swine/Chachoengsao/NIAH5... 32.2 11
dbj|AB434320.1| Influenza A virus (A/swine/Chonburi/NIAH589/2... 32.2 11
dbj|AB434312.1| Influenza A virus (A/swine/Chonburi/NIAH977/2... 32.2 11
dbj|AB434304.1| Influenza A virus (A/swine/Chonburi/NIAH9469/... 32.2 11
dbj|AB434296.1| Influenza A virus (A/swine/Ratchaburi/NIAH550... 32.2 11
gb|EU798784.1| Influenza A virus (A/swine/Korea/Asan04/2006(H... 32.2 11
gb|EU798783.1| Influenza A virus (A/swine/Korea/JL04/2005(H1N... 32.2 11
gb|EU798781.1| Influenza A virus (A/swine/Korea/JL01/2005(H1N... 32.2 11
gb|EU798780.1| Influenza A virus (A/swine/Korea/Hongsong2/200... 32.2 11
gb|EU296605.1| Influenza A virus (A/swine/Chonburi/06CB2/2006... 32.2 11
gb|EU296603.1| Influenza A virus (A/swine/Chonburi/05CB1/2005... 32.2 11
gb|EU296601.1| Influenza A virus (A/swine/Chachoengsao/NIAH58... 32.2 11
gb|EU296599.1| Influenza A virus (A/swine/Chonburi/NIAH589/20... 32.2 11
gb|CY026427.1| Influenza A virus (A/swine/Jamesburg/1942(H1N1... 32.2 11
gb|CY022469.1| Influenza A virus (A/swine/Kansas/3228/1987(H1... 32.2 11
gb|EF101749.1| Influenza A virus (A/Thailand/271/2005(H1N1)) ... 32.2 11
gb|DQ431990.1| Influenza A virus (A/swine/Taiwan/CO935/2004(H... 32.2 11
gb|DQ447187.1| Influenza A virus (A/swine/Taiwan/CO935/2004(H... 32.2 11
gb|AY852271.1| Influenza A virus (A/swine/Guangdong/711/2001(... 32.2 11
gb|U80950.1|IAU80950 Influenza A virus hemagglutinin (HA) gen... 32.2 11
gb|U72666.1|IAU72666 Influenza A virus hemagglutinin (HA) gen... 32.2 11Comment
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Re: Denmark Tamiflu Resistant Sequence Released
NJ isolate above is from Fort Dix (1976 swine flu outbreak).Comment
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Re: Denmark Tamiflu Resistant Sequence Released
PB2 travel log
gb|GQ160588.1| Influenza A virus (A/Virginia/04/2009(H1N1)) s... 38.2 0.19Comment
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Re: Denmark Tamiflu Resistant Sequence Released
PB1 travel log
dbj|AB441949.1| Influenza A virus (A/Sendai-H/271/2007(H3N2))... 32.2 11
dbj|AB441947.1| Influenza A virus (A/Sendai-H/F150/2007(H3N2)... 32.2 11
gb|CY031569.1| Influenza A virus (A/California/UR06-0588/2007... 32.2 11
gb|CY030211.1| Influenza A virus (A/Virginia/UR06-0605/2007(H... 32.2 11
gb|CY030203.1| Influenza A virus (A/Illinois/UR06-0600/2007(H... 32.2 11
gb|CY026201.1| Influenza A virus (A/California/UR06-0589/2007... 32.2 11
gb|CY025865.1| Influenza A virus (A/Alabama/UR06-0545/2007(H3... 32.2 11
gb|CY025841.1| Influenza A virus (A/New York/UR06-0373/2007(H... 32.2 11Comment
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Re: Denmark Tamiflu Resistant Sequence Released
NP travel log
dbj|AB268554.2| Influenza A virus (A/duck/Mongolia/47/2001(H7... 40.1 0.047
gb|CY036158.1| Influenza A virus (A/long-tailed shrike/Hong K... 40.1 0.047
gb|CY031159.1| Influenza A virus (A/gray teal/Western Austral... 40.1 0.047
gb|CY024789.1| Influenza A virus (A/chicken/Victoria/1976(H7N... 40.1 0.047
gb|CY005727.1| Influenza A virus (A/wedge-tailed shearwater/W... 40.1 0.047
gb|CY005720.1| Influenza A virus (A/sooty tern/Western Austra... 40.1 0.047
gb|CY005408.1| Influenza A virus (A/wedge-tailed shearwater/W... 40.1 0.047
gb|CY005742.1| Influenza A virus (A/duck/NZL/164/1976(H11N3))... 40.1 0.047
gb|CY005737.1| Influenza A virus (A/duck/NZL/160/1976(H1N3)) ... 40.1 0.047
gb|CY005694.1| Influenza A virus (A/black duck/AUS/4045/1980(... 40.1 0.047
gb|CY005682.1| Influenza A virus (A/gray teal/AUS/3/1979(H4N6... 40.1 0.047
gb|CY005674.1| Influenza A virus (A/grey teal/Australia/2/197... 40.1 0.047
gb|AY623834.1| Influenza A virus (A/swine/Pingtung/199-2/2002... 40.1 0.047
gb|M30761.1|FLANPS Influenza A virus (A/grey teal/Australia/2... 40.1 0.047Comment
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Re: Denmark Tamiflu Resistant Sequence Released
It is worth noting that PB1 in pandemic swine H1N1 is human H3N2 from 1993. The travel log is of human H3N2 from 2007.Originally posted by niman View Post
This is NOT a coincidence and NOT due to "random mutation".Comment
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Re: Denmark Tamiflu Resistant Sequence Released
Niman could you please post a short summary for us who don?t understand this kind of information? meaning, what does it mean and what is the consequenses? is this a mutation we should be worried about?
thanks
TheforeignerComment
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Re: Denmark Tamiflu Resistant Sequence Released
NS travel log
gb|GQ160590.1| Influenza A virus (A/Virginia/04/2009(H1N1)) s... 38.2 0.19Comment
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Re: Denmark Tamiflu Resistant Sequence Released
NS travel log matchs PB2 travel logComment
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Re: Denmark Tamiflu Resistant Sequence Released
The PB1 travel log is the only newly acquired polymorphism, A178T, in the PB1 sequence. I suspect it is nonsynomous because it is a transversion (swaps a purine for a pyrimidine) but haven't looked yet. I believe the donor sequences is Brisbane/10 (but also haven't looked yet). The polymorphisms represents an "upgrade" so to speak (adding a human 2007 polymorphism to a 1993 sequence).Originally posted by theforeigner View PostComment
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Re: Denmark Tamiflu Resistant Sequence Released
No new polymorphisms in PA or MP.Comment
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Re: Denmark Tamiflu Resistant Sequence Released
Denmark has also released two companion sequences
A/Denmark/523/2009
A/Denmark/524/2009Comment
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