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Re: H5N1 returns to Germany
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Re: H5N1 returns to Germany
CommentaryOriginally posted by niman View Post
Endemic H5N1 Confirmed in Saxony Germany
Recombinomics Commentary 15:20
October 20, 2008
8.10. confirmation AI H5N1 by national reference lab, sequencing ongoing ? first results indicated the presence of a HPAIV almost identical to the strain found in a tufted duck (Aythya fuligula) from the lake of Bautzen in 2006
The above comments from a report on the H5N1 and H5N3 outbreaks in Saxony strongly suggest that H5N1 is endemic in Germany and is circulating largely undetected. In 2006 there were three distinct sub-clades circulating in Germany, including one sub-clade which was also found in Switzerland, France, and the Czech Republic. Although this sub-clade was reported in multiple countries in early 2006, it has not been reported since. The recent detection of this sub-clade in 25 free range birds in Saxony indicates it has been circulating undetected in Germany for over 2 years.
Reports of H5N1 from Germany since the summer of 2007 have been limited to outbreak involving sub-clade 2.2.3, which is easily distinguished from the 2006 isolates in Germany.
However, in early 2007 clade 2.2.3 isolates began acquiring NA G743A, which had been limited to the 2006 isolates that included A/tufted duck/Germany/R1240/06. G743A was appended onto multiple clade 2.2 genetic backgrounds in early 2007, and clade 2.2.3 with G743A was widely detected in Europe after the summer 2007 outbreaks.
The detection of 2008 isolates with sequence closely related to the tuft duck isolate above suggests that this sub-clade may have been circulating undetected in late 2006 / early 2007 and may have been the donor sequence for the acquisition of G743A.
The detection of the 2006 sequence in 2008 strongly suggests that H5N1 is endemic in Germany and throughout Europe.
."The next major advancement in the health of American people will be determined by what the individual is willing to do for himself"-- John Knowles, Former President of the Rockefeller FoundationComment
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Re: H5N1 returns to Germany
Source: http://www.lvz-online.de/aktuell/content/76419.html
Google translation:
Bird flu at Gut M?lkau: All poultry flock killed
Leipzig. The bird flu has reached Stadtgut M?lkau. It is thus the Leipzig zoo after the second case in the fair city. The Veterinary Office confirmed that during a routine inspection in early October when three animals are low pathogenic influenza viruses were detected. Certainty have an investigation by the Friedrich-Loeffler-Institute on the island of Riems brought. Although it is less dangerous variant of the disease that had All 106 animals on the farm had been killed. "The legislature writes the way," said office manager Gabriela Leupold compared LVZ online. The inventory included 59 ducks, 17 geese, 24 chickens and 7 quails.
Has infected the poultry M?lkau probably in wild birds. They carry the H5 virus is often in and give it further. The experts are infected Hoftiere that although clinically healthy and showed no disease characteristics. The danger exists but is that the virus pathogenic type H5N1 to mutate. These variant of avian flu can also be dangerous for humans and lead to death.
According to a report to the Federal Ministry of Food, Agriculture and Consumer Protection, the LVZ online exists, is the case of Stadtgut M?lkau since the 9th October known. Previously, he was for the people, however, kept under lock. "With the low pathogenic variant is no information requirement," said a spokesman for the Dresdner Social Ministry.
Authorities would only internally by the official veterinarian, a data chain ausgl?st by the National Directorate of the Ministry of State until the federal government to Berlin rich. The state economy ministry directs a spokeswoman that the messages from the authorities network "Animal News" in addition to the European Union.
The Leipzig veterinary office head Leupold looks through the second case within a few days with no increased risk for Leipzig's poultry farmers in the vicinity of the goods M?lkau are just a breeder. Its stock had long been investigated, without proof of influenza viruses.
In the last week of the Leipzig Zoo had indicated that his investment is also less dangerous type of bird flu occurred. A yellow-beak, a Sichelente and a Brandgans therefore had to be killed. Also here are wild animals as a suspected polluter.
All birds in the zoo must now begin for three weeks remain in the stall. Then again samples taken from 60 animals. "Are all the negative, can be transferred back to normalcy," said Stefan Barton, spokesman for the National Directorate of Leipzig. Zoodirektoren Joerg Junold asks for understanding that these precautionary measures because some birds that would otherwise live in open-air enclosures, such as ostriches, pelicans and several species of water birds Not be seen.
In marker near Goerlitz was about two weeks before even the most dangerous variant of the bird flu outbreak at a duck. Other cases, according to council late last week not reported. In a radius of 50 kilometers, however, there is a strict requirement to keep birds indoors. Of these, the county Goerlitz, as well as parts of the counties Bautzen and Saxon Switzerland-east affected. In this restricted zone is also an import and export ban on poultry products.
Matthias Roth, LVZ-Online/L.P./dpa
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Re: H5N1 returns to Germany
26.04.2006 SN Bautzen Bautzen (Neumalsitz) Wildente
34 > A/tufted duck/Germany/R1240/06
3 strains in Germany 2006:
(1)Baltic
(2)Bavaria (edit: also Switzerland,Czech,France)
(3)Italy/Slowakia/Austria/Czech/Slovenia
the tufted duck if drom (2) although Saxony, not Bavaria and close to the current location.I'm interested in expert panflu damage estimates
my current links: http://bit.ly/hFI7H ILI-charts: http://bit.ly/CcRgTComment
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Re: H5N1 returns to Germany
The Bavaria sub-clade was in Switzerland, czech republic, and france in earlyy 2006. All had g743a.Originally posted by gsgs View PostComment
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Re: H5N1 returns to Germany
AVIAN INFLUENZA (107): GERMANY (SAXONY), HPAIV & LPAIV
************************************************** ****
A ProMED-mail post
<http://www.promedmail.org>
ProMED-mail is a program of the
International Society for Infectious Diseases
<http://www.isid.org>
Date: Wed 22 Oct 2008
From: Martin Beer <Martin.Beer@fli.bund.de>
Highly pathogenic avian influenza virus H5N1 has been detected in
clinically healthy ducks in a mixed poultry holding in Saxony,
Germany. 30 oropharyngeal swabs from geese and ducks had been sampled
for routine monitoring purposes. A single duck tested positive for
HPAIV H5N1, and the cleavage site sequence was confirmed as
GERRRKKR*GLF on 9 Oct 2008. Subsequently, 157 additional duck samples
were obtained and revealed a further 24 H5N1 positives, of which 9
had sufficient viral loads to be confirmed as HPAIV. All poultry were
culled. No more cases have been detected up to now in contact farms
or in wild bird samples obtained from this region.
Preliminary sequence and phylogenetic analysis of the HA gene from 4
duck samples gave evidence for a representative of a cluster 2.2
sublineage which had been detected in spring 2006 in Southern
Germany. In fact, a virus from a tufted duck (R1240/06; Starick et
al., 2008) found in 2006 40 km away from the present outbreak
location is very closely related. So far, there are no records for
the presence of viruses of this sublineage after May 2006 in Germany
and Europe. In summary, the data suggest a very recent introduction
of HPAIV H5N1 into this farm from an as yet unknown source.
In parallel, LPAIV H5 infections have been detected in waterfowl in
the zoo of Leipzig, Saxony and in a small mixed poultry holding near
Leipzig. These viruses turned out to be typical representatives of
Eurasian H5 LPAIV currently also detected in aquatic wild birds in
Germany. Poultry at the LPAIV-affected holdings had been culled as
well.
[Elke Starick (Senior Scientist, OIE and National Reference
Laboratory AI) Dr. Christian Grund (Deputy Head OIE and National
Reference Laboratory AI) Dr. Timm Harder (Head OIE and National
Reference Laboratory AI) Dr. Martin Beer (Head Institute of
Diagnostic Virology, Friedrich-Loeffler-Institut) Prof. Dr. Thomas C.
Mettenleiter (President, Friedrich-Loeffler-Institut)]
--
Communicated by:
Dr. Martin Beer
Head Institute of Diagnostic Virology
Friedrich-Loeffler-Institut
Am Suedufer 10
17493 Greifswald-Insel Riems
Germany
<Martin.Beer@fli.bund.de>
[see also:
Avian influenza (105): Germany (Saxony), LPNAI H5N3, Update 20081018.3303
Avian influenza (104): Germany (Saxony), OIE, LPNAI H5N3, RFI 20081018.3296]
.................................................. .arn/msp/jwComment
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Re: H5N1 returns to Germany
Commentary
Comment
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Re: H5N1 returns to Germany
Asymptomatic H5N1 in Germany Raises Surveillance Concerns
Recombinomics Commentary 12:55
October 27, 2008
A single duck tested positive for HPAIV H5N1, and the cleavage site sequence was confirmed as GERRRKKR*GLF on 9 Oct 2008. Subsequently,157 additional duck samples were obtained and revealed a further 24 H5N1 positives, of which 9 had sufficient viral loads to be confirmed as HPAIV.
Preliminary sequence and phylogenetic analysis of the HA gene from 4
duck samples gave evidence for a representative of a cluster 2.2
sublineage which had been detected in spring 2006 in Southern
Germany. In fact, a virus from a tufted duck (R1240/06; Starick et
al., 2008) found in 2006 40 km away from the present outbreak
location is very closely related.
The above comments on H5N1 in asymptomatic free range ducks in Saxony, Germany, leaves little doubt that the sub-clade has been maintained for two years in an undetected reservoir in Germany. This sub-clade was found in wild birds in southern Germany, Switzerland, the Czech Republic and France in early 2006. The sequence was readily distinguished fro other clade 2.2 sub-clades in Europe, by virtue of a number of polymorphisms, including NA G743A. However, after the outbreaks in early 2006, there were no reports of this sub-clade in poultry or wild birds.
Although it is possible that the sub-clade was maintained in domestic poultry, a wild bird reservoir is more likely because of the failure to detect this sub-clade during routine poultry testing between 2005-2008. As noted above, the infected birds were asymptomatic and most birds positive by PCR did not have sufficient levels of RNA to yield sequencing data, which again raises surveillance questions.
Detection of H5N1 in asymptomatic birds has been problematic. Almost all detection in Europe has been in dead or dying birds. These detection failures have been noted worldwide. A December, 2005 from a teal in Egypt yielded HA and NA sequence data for clade 2.2, but the sequence creation required multiple RNA extractions and virus was not isolated. However, the sequence was closely related to clade 2.2 from Austria, even though no EU country had acknowledged H5N1 infections in 2005. The first confirmed positives in the EU were in early 2006, once again supporting the view that surveillance approaches generally produce false negatives for live wild birds transporting and transmitting H5N1.
The failures have been confirmed in experimentally infected wild birds. These birds, when infected with lower levels of virus, yield H5N1 positive PCR in nasopharyngeal swabs when cloacal swabs are negative. Moreover, like the above data, most of the samples that were PCR positive failed to lead to virus isolation. Isolation was generally limited to a single 24 hour time frame several days after infection. Thus, experimental birds that are shedding H5N1 usually fail to yield virus, and most samples produce false negatives.
These false negatives have led to earlier pronouncements on the demise of H5N1 in wild birds. In June, 2007, wild birds in Europe was declared free of H5N1 based on negative data generated by a consortium of bird conservation groups collecting samples for FAO. Within minutes of this pronouncement, the Czech Republic reported in H5N1 in domestic poultry. This announcement was followed by detection of H5N1 in wild birds in the Czech Republic, Germany, and France. These isolates were clade 2.2.3, which were readily distinguished from the various clade 2.2 sub-clade reported in Europe in 2006. The strain had evolved over the summer of 2006 in Mongolia at Uvs Lake. The appearance throughout Europe in the summer of 2007, when long range migration was minimal, suggested this sub-clade had also entered Europe undetected in the 2006/2007 season, and was not reported until the summer of 2007. These outbreaks were followe3d by widespread detection of the Uvs Lake version of clade 2.2.3 in Europe in the 2007/2008 season.
Thus, current surveillance methods largely miss H5N1 in asymptomatic birds. The most recent data define a sub-clade circulating undetected in Europe between 2006 and 2008. These assays also failed to detect H5N1 in EU countries prior to early 2006, and also failed to detect the entry of clade 2.2.3 in late 2006, early 2007.
These detection failures remain a cause for concern.Comment
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Re: H5N1 returns to Germany
tufted duck (R1240/06; Starick et
al., 2008) found in 2006 40 km away from the present outbreak
location is very closely related.
> very closely related
ahh.
How very ? Just the number of differences, please.
There are 2.5 years between those
so "very", that we could assume the virus was frozen ?
in 1 or 2 years they might tell us.I'm interested in expert panflu damage estimates
my current links: http://bit.ly/hFI7H ILI-charts: http://bit.ly/CcRgTComment
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Re: H5N1 returns to Germany
Please. The "frozen" nonsense (with no support) is getting old.Originally posted by gsgs View PostComment
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Re: H5N1 returns to Germany
CommentaryOriginally posted by niman View Post
Asymptomatic H5N1 in Germany Raises Surveillance Concerns
Recombinomics Commentary 12:55
October 27, 2008
A single duck tested positive for HPAIV H5N1, and the cleavage site sequence was confirmed as GERRRKKR*GLF on 9 Oct 2008. Subsequently,157 additional duck samples were obtained and revealed a further 24 H5N1 positives, of which 9 had sufficient viral loads to be confirmed as HPAIV.
Preliminary sequence and phylogenetic analysis of the HA gene from 4 duck samples gave evidence for a representative of a cluster 2.2 sublineage which had been detected in spring 2006 in Southern Germany. In fact, a virus from a tufted duck (R1240/06; Starick et al., 2008) found in 2006 40 km away from the present outbreak location is very closely related.
The above comments on H5N1 in asymptomatic free range ducks in Saxony, Germany, leaves little doubt that the sub-clade has been maintained for two years in an undetected reservoir in Germany. This sub-clade was found in wild birds in southern Germany, Switzerland, the Czech Republic and France in early 2006. The sequence was readily distinguished from other clade 2.2 sub-clades in Europe, by virtue of a number of polymorphisms, including NA G743A. However, after the outbreaks in early 2006, there were no reports of this sub-clade in poultry or wild birds.
Although it is possible that the sub-clade was maintained in domestic poultry, a wild bird reservoir is more likely because of the failure to detect this sub-clade during routine poultry testing between 2005-2008. As noted above, the infected birds were asymptomatic and most birds positive by PCR did not have sufficient levels of RNA to yield sequencing data, which again raises surveillance questions.
Detection of H5N1 in asymptomatic birds has been problematic. Almost all detection in Europe has been in dead or dying birds. These detection failures have been noted worldwide. A December, 2005 from a teal in Egypt yielded HA and NA sequence data for clade 2.2, but the sequence creation required multiple RNA extractions and virus was not isolated. However, the sequence was closely related to clade 2.2 from Austria, even though no EU country had acknowledged H5N1 infections in 2005. The first confirmed positives in the EU were in early 2006, once again supporting the view that surveillance approaches generally produce false negatives for live wild birds transporting and transmitting H5N1.
The failures have been confirmed in experimentally infected wild birds. These birds, when infected with lower levels of virus, yield H5N1 positive PCR in nasopharyngeal swabs when cloacal swabs are negative. Moreover, like the above data, most of the samples that were PCR positive failed to lead to virus isolation. Isolation was generally limited to a single 24 hour time frame several days after infection. Thus, experimental birds that are shedding H5N1 usually fail to yield virus, and most samples produce false negatives.
These false negatives have led to earlier pronouncements on the demise of H5N1 in wild birds. In June, 2007, wild birds in Europe was declared free of H5N1 based on negative data generated by a consortium of bird conservation groups collecting samples for FAO. Within minutes of this pronouncement, the Czech Republic reported in H5N1 in domestic poultry. This announcement was followed by detection of H5N1 in wild birds in the Czech Republic, Germany, and France. These isolates were clade 2.2.3, which were readily distinguished from the various clade 2.2 sub-clade reported in Europe in 2006. The strain had evolved over the summer of 2006 in Mongolia at Uvs Lake. The appearance throughout Europe in the summer of 2007, when long range migration was minimal, suggested this sub-clade had also entered Europe undetected in the 2006/2007 season, and was not reported until the summer of 2007. These outbreaks were followed by widespread detection of the Uvs Lake version of clade 2.2.3 in Europe in the 2007/2008 season.
Thus, current surveillance methods largely miss H5N1 in asymptomatic birds. The most recent data define a sub-clade circulating undetected in Europe between 2006 and 2008. These assays also failed to detect H5N1 in EU countries prior to early 2006, and also failed to detect the entry of clade 2.2.3 in late 2006, early 2007.
These detection failures remain a cause for concern.
."The next major advancement in the health of American people will be determined by what the individual is willing to do for himself"-- John Knowles, Former President of the Rockefeller FoundationComment
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Re: H5N1 returns to Germany
Source: http://www.lvz-online.de/aktuell/content/77963.html
Google translation:
New suspected bird flu: dead swan found near Goerlitz
Goerlitz / Dresden. A county in Goerlitz discovered dead swan is being tested for bird flu on. The bird was at the weekend near a sawmill in Dauban / Hohendubrau been found, told the council on Tuesday. In the Saxon Investigation Institute in Dresden, he will now concentrate on dangerous influenza virus type H5N1 investigated. "A dead swan is nothing extraordinary about this season, but we are aware," said a spokeswoman. The result would be expected shortly.
In early October was on a Gefl?gelhof marker in the village at Goerlitz outbreak of bird flu. First it was the only one duck was infected. According to the Ministry of Social Affairs in Dresden was the virus, but more than 20 other ducks from the collection have been demonstrated. Around 1400 ducks and geese were killed. The H5N1 virus affects birds mainly chickens and turkeys, but also water birds such as ducks and geese.
German
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English
TranslateComment
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Re: H5N1 returns to Germany
I found another part underneath this article; also translate via Google but with some alternation due to very weard transl.
In a radius of 50 kilometers lockduty still exists, according to the townhall people until 13 November. When the dead swan shows no bird flu, the lock districts repealed. So far, the county Goerlitz, as well as parts of the counties Bautzen and Saxon Switzerland-east will be affected. Even in parts of Brandenburg as well as Poland and the Czech Republic must be caged poultry. In this area there is also an import and export ban on poultry products.
As for a suspected bird flu in most cases the entire poultry stock of precaution is killed, the high economic damage to the breeder mean. Bird flu was the last in December 2007 in Brandenburg erupted. For people is an infection with the virus possible, but very rarely.Comment
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Re: H5N1 returns to Germany
credits gsgs
machinetranslation
Dead swan in Dauban not with bird flu virus
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Goerlitz - The weekend in Dauban Goerlitz in the county discovered dead swan is not the bird flu virus. It announced on Wednesday morning with the county Goerlitz. The Saxon Investigation Institute in Dresden conducted test on the dangerous influenza virus type H5N1 was negative.Comment
Comment