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Carbapenem-resistant bacteria found in 2016 Olympic rowing location in Brazil
September 6, 2014
WASHINGTON, D.C. ? Data presented here at ICAAC 2014 indicate the presence of carbapenem-resistant bacteria in the Rodrigo de Freitas Lagoon in Rio de Janeiro, which is the designated venue for rowing events during the 2016 Olympics.
The researchers also found bacteria producing the Klebsiella pneumoniae carbapenamase in the Carioca River, which flows through several neighborhoods in Rio de Janeiro, and into Flamengo Beach, according to the researchers from the Oswaldo Cruz Foundation.
The researchers obtained water samples from four locations on the Rodrigo de Freitas Lagoon and five locations on the Carioca River. They performed PCR and sequencing of 16S rDNA to identify bacterial morphotypes, including those with the blaKPC gene.
There were 157 isolates identified as resistant to at least one carbapenem of three tested: 42% were taken from the Rodrigo de Freitas Lagoon and 58% from the Carioca River. On an ertapenem hydrolysis test, 81% of the isolates from the Carioca River and 19% from the Rodrigo de Freitas Lagoon demonstrated carbapenemase activity.
Thirty-three isolates in the Enterobacteriaceae and Aeromonas spp., taken from three points of the Carioca River, had the blaKPC-2 gene and all of those had a partial Tn4401 structure. They found that only 28% of these isolates showed tnpA, ISKpn6 and ISKpn7 together. In addition, XbaI-macrorestriction found 18 different patterns.
?The presence of isolates resistant to carbapenems in [these bodies of water] highlights that aquatic environments can be a reservoir of resistance genes,? the researchers wrote. ?The utilization of these waters could facilitate a global spread of this and other important antimicrobial resistance genes.?
Araujo C. Interscience Conference on Antimicrobial Agents and Chemotherapy; Sept. 5-9, 2014; Washington, D.C.
Healio.com
September 6, 2014
WASHINGTON, D.C. ? Data presented here at ICAAC 2014 indicate the presence of carbapenem-resistant bacteria in the Rodrigo de Freitas Lagoon in Rio de Janeiro, which is the designated venue for rowing events during the 2016 Olympics.
The researchers also found bacteria producing the Klebsiella pneumoniae carbapenamase in the Carioca River, which flows through several neighborhoods in Rio de Janeiro, and into Flamengo Beach, according to the researchers from the Oswaldo Cruz Foundation.
The researchers obtained water samples from four locations on the Rodrigo de Freitas Lagoon and five locations on the Carioca River. They performed PCR and sequencing of 16S rDNA to identify bacterial morphotypes, including those with the blaKPC gene.
There were 157 isolates identified as resistant to at least one carbapenem of three tested: 42% were taken from the Rodrigo de Freitas Lagoon and 58% from the Carioca River. On an ertapenem hydrolysis test, 81% of the isolates from the Carioca River and 19% from the Rodrigo de Freitas Lagoon demonstrated carbapenemase activity.
Thirty-three isolates in the Enterobacteriaceae and Aeromonas spp., taken from three points of the Carioca River, had the blaKPC-2 gene and all of those had a partial Tn4401 structure. They found that only 28% of these isolates showed tnpA, ISKpn6 and ISKpn7 together. In addition, XbaI-macrorestriction found 18 different patterns.
?The presence of isolates resistant to carbapenems in [these bodies of water] highlights that aquatic environments can be a reservoir of resistance genes,? the researchers wrote. ?The utilization of these waters could facilitate a global spread of this and other important antimicrobial resistance genes.?
Araujo C. Interscience Conference on Antimicrobial Agents and Chemotherapy; Sept. 5-9, 2014; Washington, D.C.
Healio.com
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