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  • AFRICAN SWINE FEVER - GEORGIA

    Date: Thu 7 Jun 2007
    Source: OIE press release [edited]
    <http://oie.int/eng/press/en_070607.htm>


    Georgia has officially notified OIE (Office International des Epizooties;
    World Organisation for Animal Health) of 11 outbreaks of African swine
    fever (ASF) in pigs. This is the 1st ever occurrence of the disease in
    northern Europe [location described elsewhere as south east Europe/south
    west Asia.
    Mod.SH].

    Planning is underway to rapidly deploy an OIE/FAO/EC expert mission to Georgia.

    ASF is generally prevalent and endemic in countries of sub-Saharan Africa.
    In Europe, ASF is still endemic in specific areas in Italy (Sardinia). No
    reported outbreaks of ASF in other European countries have occurred since
    1999 in Portugal.

    On 17 May 2007, Georgia 1st notified the OIE of several outbreaks
    attributed to porcine circovirus, which causes wasting disease in young
    pigs. Tests carried out on samples submitted to the OIE Reference
    Laboratory in Pirbright, United Kingdom confirmed the presence of African
    swine fever virus. The source of the infection is currently under
    investigation.

    Classical disease control measures have been implemented by the Georgian
    authorities, including the culling of animals and isolation of infected
    properties. A total of 20 000 susceptible pigs from villages and private
    farms were destroyed in 10 regions across the country. (See official report
    of the country to the OIE at <http://www.oie.int/>).

    An effective and rapid control of the outbreak will avoid ASF from
    establishing a foothold and become endemic in the region.

    Emerging transboundary diseases
    -------------------------------
    African swine fever (ASF) is a serious transboundary animal disease highly
    lethal to pigs with the propensity for rapid and unanticipated
    international spread and subsequent significant economic, trade and food
    security consequences.

    The rapid detection, notification and response in Georgia coupled with
    international cooperation is crucial for the containment of spread and
    eventual exclusion of this disease from the area. [For the list of OIE
    reference labs, see commentary below. - Mod.AS]

    The OIE continues to highlight the importance of having effective
    Veterinary Services able to early detect and respond rapidly to an
    unexpected event such as this. Only an effective national surveillance
    system and transparency in reporting can ensure good control policies are
    implemented.

    Since the bluetongue outbreak in The Netherlands in August 2006, this ASF
    outbreak in Georgia is the 2nd appearance of an emerging disease for
    Northern Europe in a short time.

    Background information
    ----------------------
    African swine fever is a tickborne viral disease -- affecting only species
    of the pig family -- which can result in very high mortalities. Infection
    can occur via contact with infected ticks, and direct transmission is
    important in domestic pigs, as the virus is shed in high concentration in
    excretions and secretions of infected animals. The virus can be spread
    through feeding with garbage containing infected meat. Specific hosts
    include pigs, warthogs, bush pigs, European wild boars, American wild pigs,
    and giant forest hogs. African warthogs and bushpigs are considered natural
    reservoirs. There is not yet an effective vaccine available or effective
    treatment against ASF.


    The disease poses no danger to human health.

    --
    Communicated by:
    Maria Zampaglione
    Head, Communication Unit
    OIE
    Organisation Mondiale de la Sante Animale
    World Organisation for Animal Health
    12, Rue de Prony
    75017 Paris, France
    <m.zampaglione@oie.int>

    ******
    [2]
    Date: Thu 7 Jun 2007
    Source: RIA Novosti, Russian News and Information Agency [edited]
    <http://en.rian.ru/russia/20070607/66847462.html>


    Russia's food safety watchdog was reported in this story as saying Thursday
    [7 Jun 2007] it had imposed restrictions on meat imports from Georgia after
    an outbreak of African swine fever registered in the South Caucasus
    country. Rosselkhoznadzor said in a statement that "outbreaks of African
    swine fever in Georgia have been registered in 10 populated areas,
    including Tbilisi. In these areas, 20 000 infected pigs have been
    destroyed. Georgia has imposed restrictions on the movement of cattle;
    contaminated premises have been disinfected, and a quarantine regime has
    been introduced in the disease-stricken areas."


    --
    communicated by:
    ProMED-mail <promed@promedmail.org>

    [The identification of an exotic disease, when encountered for the 1st time
    in a previously free country, is a tricky business. There are examples of
    countries where such disease was evolving for extended periods before
    identified and definitely diagnosed. The Georgian situation is not
    exceptional. The authorities seem to have suspected ASF already at early
    stages of the outbreak, in April 2007, but, as stated under
    "epidemiological comments" in their initial "immediate notification" to the
    OIE of 17 May 2007, "pathological material from 150 animals was tested in
    the laboratory of the Ministry of Agriculture of Georgia. Agents of
    secondary bacterial infections (Pasteurellosis, erysipelas, salmonellosis)
    were detected. The presence of African swine fever and classical swine
    fever was excluded." Their transparency and their initiative to send
    suspected material to the OIE reference laboratory in Pirbright for
    advanced testing are commendable.

    For detailed information on ASF diagnostic methods, subscribers are
    referred to chapter 2.1.12 of OIE's Manual of Diagnostic Tests and Vaccines
    for Terrestrial Animals (updated 23 Jul 2004), available at
    <http://oie.int/eng/normes/mmanual/A_00035.htm>. We hereby copy the summary
    of the said extensive review:

    "African swine fever (ASF) is an infectious disease of domestic and wild
    pigs that affects animals of all breeds and ages, and which is caused by a
    virus that produces a range of syndromes. Acute disease is characterized by
    high fever, hemorrhages in the reticuloendothelial system, and a high
    mortality rate. Infectious virus can survive for several months in fresh
    and salted dried-meat products.


    "ASF virus is the only member of the _Asfarviridae_ family.

    "Laboratory diagnostic procedures for ASF fall into 2 groups: the 1st
    contains the tests for virus isolation and the detection of virus antigens
    and genomic DNA, while the 2nd contains the tests for antibody detection.
    The selection of the tests to be carried out depends on the disease
    situation in the area or country.

    "In countries free from ASF but suspecting its presence, the laboratory
    diagnosis must be directed towards isolation of the virus by simultaneously
    carrying out the inoculation of pig leukocyte or bone marrow cultures, the
    detection of antigen in smears or cryostat sections of tissues by
    fluorescent antibody test (FAT) and, where possible, the detection of
    genomic DNA by polymerase chain reaction (PCR). The detection of antibodies
    in tissue fluids by the indirect fluorescent antibody test should also be
    carried out at the same time.

    "Identification of the agent: Tissues submitted from suspected cases of
    disease in the field should be examined for specific antigen by the FAT on
    smears or cryostat sections and for the presence of virus by inoculation of
    primary pig leukocyte cultures, which are examined daily for hemadsorption
    and cytopathic effects. The cells from negative cultures are examined for
    antigen by FAT and subinoculation into fresh leukocyte cultures.

    "The PCR can be used to detect virus genome in tissues and is especially
    useful if these are unsuitable for virus isolation and antigen detection.

    "In doubtful cases, the material is passaged, and the procedures described
    above are repeated.

    "Serological tests: Where the disease is endemic, or where a primary
    outbreak is caused by a strain of low virulence, the investigation of new
    outbreaks should include the detection, using the enzyme-linked
    immunosorbent assay, of specific antibodies in serum or extracts of the
    tissues submitted.

    "Requirements for vaccines and diagnostic biologicals: At present, there is
    no vaccine for ASF."

    OIE has designated the following 3 ASF reference experts and laboratories:
    1. Dr JM Sanchez-Vizcaino, Centro de Investigacion en Sanidad Animal,
    Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentari
    (CISA-INIA), 28130 Valdeolmos, (Madrid), SPAIN <diagnost@inia.es>.
    2. Dr Chris Oura, Institute for Animal Health, Pirbright Laboratory, Ash
    Road, Pirbright, Woking, Surrey GU24 ONF UNITED KINGDOM
    <chris.oura@bbsrc.ac.uk>
    3. Ms Alison Lubisi, Onderstepoort Veterinary Institute, Exotic Diseases
    Division, Private Bag X5, Onderstepoort 0110, SOUTH AFRICA
    <Lubisia@arc.agric.za>.

    Countries which suspect ASF are encouraged to contact one of the reference
    laboratories for assistance. - Mod.AS]

    CSI:WORLD http://swineflumagazine.blogspot.com/

    treyfish2004@yahoo.com

  • #2
    Re: AFRICAN SWINE FEVER - GEORGIA

    Date: Fri 8 Jun 2007
    From: Chris Oura
    <Chris.Oura@bbsrc.ac.uk>


    Information on the genomic characterization of African swine fever virus
    isolate from Georgia

    -----------------------------------------------------
    African swine fever virus (ASFV) samples were received from Georgia on 2
    Jun 2007 by the ASFV OIE Reference Laboratory at the Institute for Animal
    Health Pirbright Laboratory UK and were confirmed positive on 3 Jun 2007
    and reported to both the Georgians and OIE on 4 Jun 2007.

    Sequence analysis of 2 genome fragments (3' end of the p72 gene and central
    variable region of the B602L gene) has now been completed. This analysis
    showed that the Georgia 2007 ASFV outbreak was caused by the introduction
    of a virus belonging to a genotype (Genotype II, Lubisi et al, 2005)
    previously identified to contain isolates obtained from domestic pigs,
    isolated between 1993 and 2002, in Mozambique and Zambia in the eastern
    part of southern Africa and isolates introduced into Madagascar when ASFV
    spread to the island in 1998.

    Since very few ASFV samples are submitted for genotyping it is possible
    that viruses belonging to this genotype may be more widespread. However, it
    seems likely that the source of infection is from the eastern side of
    southern Africa rather than west or central Africa or Sardinia.

    --
    communicated by:
    Dr Chris Oura and Dr Linda Dixon
    OIE Reference lab for ASF
    Institute for Animal Health
    Pirbright, UK
    <Chris.Oura@bbsrc.ac.uk>

    [Drs Oura and Dixon are gratefully acknowledged for this 1st-hand,
    authoritative information. The OIE ASF reference laboratory at Pirbright
    deserves to be warmly commended for the expediency of its handling of this
    case; notably, the samples were received from Georgia on a Saturday (2 Jun
    2007) and the diagnosis was obtained during the same weekend, namely on
    Sunday, and promptly reported.

    It may be reminded that initial tests, carried in Georgia for ASF in
    April/May 2007, were -- reportedly -- found negative. We take the liberty
    to recommend that other countries, experiencing unusual disease occurrence
    in swine, will follow Georgia's example and submit samples for confirmation
    to one of OIE's reference laboratories even if initial tests in national
    laboratories are found negative. - Mod.AS].

    CSI:WORLD http://swineflumagazine.blogspot.com/

    treyfish2004@yahoo.com

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