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Microbiol Spectr
. 2023 Nov 16:e0259123.
doi: 10.1128/spectrum.02591-23. Online ahead of print. A sensitive and simple RT-LAMP assay for sarbecovirus screening in bats
Tony Tat-Yin Chan # 1 , Franklin Wang-Ngai Chow # 1 2 , Joshua Fung # 1 2 , Flora Ka-Kei Cheng 1 , George Chi-Shing Lo 1 , Chi-Ching Tsang 1 3 , Hayes Kam-Hei Luk 1 , Antonio Cheuk-Pui Wong 1 , Zirong He 1 , Kam Leng Aw-Yong 1 , Xueyan Liu 1 , Kwok-Yung Yuen 1 , Patrick Chiu-Yat Woo # 1 4 5 , Susanna Kar-Pui Lau # 1
Affiliations
We report the application of a colorimetric and fluorescent reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay to facilitate mass screening for sarbecoviruses in bats. The assay was evaluated using a total of 838 oral and alimentary samples from bats and demonstrated comparable sensitivity and specificity to quantitative reverse transcription PCR (qRT-PCR), with a simple setup. The addition of SYTO9, a fluorescent nucleic acid stain, also allows for quantitative analysis. The scalability and simplicity of the assay are believed to contribute to improving preparedness for detecting emerging coronaviruses by applying it to field studies and surveillance.
Keywords: RT-LAMP; SYTO9; bat screening; coronavirus; molecular detection; sarbecovirus.
. 2023 Nov 16:e0259123.
doi: 10.1128/spectrum.02591-23. Online ahead of print. A sensitive and simple RT-LAMP assay for sarbecovirus screening in bats
Tony Tat-Yin Chan # 1 , Franklin Wang-Ngai Chow # 1 2 , Joshua Fung # 1 2 , Flora Ka-Kei Cheng 1 , George Chi-Shing Lo 1 , Chi-Ching Tsang 1 3 , Hayes Kam-Hei Luk 1 , Antonio Cheuk-Pui Wong 1 , Zirong He 1 , Kam Leng Aw-Yong 1 , Xueyan Liu 1 , Kwok-Yung Yuen 1 , Patrick Chiu-Yat Woo # 1 4 5 , Susanna Kar-Pui Lau # 1
Affiliations
- PMID: 37971222
- DOI: 10.1128/spectrum.02591-23
We report the application of a colorimetric and fluorescent reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay to facilitate mass screening for sarbecoviruses in bats. The assay was evaluated using a total of 838 oral and alimentary samples from bats and demonstrated comparable sensitivity and specificity to quantitative reverse transcription PCR (qRT-PCR), with a simple setup. The addition of SYTO9, a fluorescent nucleic acid stain, also allows for quantitative analysis. The scalability and simplicity of the assay are believed to contribute to improving preparedness for detecting emerging coronaviruses by applying it to field studies and surveillance.
Keywords: RT-LAMP; SYTO9; bat screening; coronavirus; molecular detection; sarbecovirus.