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. 2025 Jun 3;12(1):934.
doi: 10.1038/s41597-025-05100-x. Wastewater dataset on the SARS-CoV-2 sublineages circulating in Central Arkansas, USA, post-COVID-19 pandemic
Volodymyr P Tryndyak 1 , Tetyana Kudlyk 1 , Patricia Shores 1 , Michelle M Vanlandingham 1 , Lisa Mullis 2 , Luísa Camacho 1 , Marli Azevedo 2 , Camila S Silva 3
Affiliations
The ability of coronaviruses to adapt to new hosts and cause widespread disease outbreaks poses a significant threat to global public health systems and economies. The severity of the COVID-19 pandemic has emphasized the importance of studying coronaviruses and monitoring them in communities. We investigated SARS-CoV-2 and its genomic changes in wastewater influent sampled from two metropolitan areas in Arkansas, USA, between April 2020 and March 2024. The data presented here are a follow up report to our previous publication on the findings from the period of April 2020 to January 2022 and show the SARS-CoV-2 variants circulating between February 2022 and March 2024. The levels of viral RNA were measured by reverse-transcription quantitative polymerase chain reaction and targeted three SARS-CoV-2 genes (encoding ORF1ab polyprotein, ORF1ab; surface glycoprotein, S-protein; and nucleocapsid phosphoprotein, N-protein). The identity and genetic diversity of the virus were investigated using amplicon-based RNA sequencing. These data provide important information on SARS-CoV-2 evolution and help to understand the occurrence of COVID-19 outbreaks in the community.
. 2025 Jun 3;12(1):934.
doi: 10.1038/s41597-025-05100-x. Wastewater dataset on the SARS-CoV-2 sublineages circulating in Central Arkansas, USA, post-COVID-19 pandemic
Volodymyr P Tryndyak 1 , Tetyana Kudlyk 1 , Patricia Shores 1 , Michelle M Vanlandingham 1 , Lisa Mullis 2 , Luísa Camacho 1 , Marli Azevedo 2 , Camila S Silva 3
Affiliations
- PMID: 40461497
- PMCID: PMC12134094
- DOI: 10.1038/s41597-025-05100-x
The ability of coronaviruses to adapt to new hosts and cause widespread disease outbreaks poses a significant threat to global public health systems and economies. The severity of the COVID-19 pandemic has emphasized the importance of studying coronaviruses and monitoring them in communities. We investigated SARS-CoV-2 and its genomic changes in wastewater influent sampled from two metropolitan areas in Arkansas, USA, between April 2020 and March 2024. The data presented here are a follow up report to our previous publication on the findings from the period of April 2020 to January 2022 and show the SARS-CoV-2 variants circulating between February 2022 and March 2024. The levels of viral RNA were measured by reverse-transcription quantitative polymerase chain reaction and targeted three SARS-CoV-2 genes (encoding ORF1ab polyprotein, ORF1ab; surface glycoprotein, S-protein; and nucleocapsid phosphoprotein, N-protein). The identity and genetic diversity of the virus were investigated using amplicon-based RNA sequencing. These data provide important information on SARS-CoV-2 evolution and help to understand the occurrence of COVID-19 outbreaks in the community.