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First published: 20 November 2022
Collin Jugler, Haiyan Sun, Katherine Nguyen, Roman Palt, Mitchell Felder, Herta Steinkellner, Qiang Chen
Summary
This study describes a novel, neutralizing monoclonal antibody (mAb), 11D7, discovered by mouse immunization and hybridoma generation, against the parental Wuhan-Hu-1 RBD of SARS-CoV-2. We further developed this mAb into a chimeric human IgG and recombinantly expressed it in plants to produce a mAb with human-like, highly homogenous N-linked glycans that has potential to impart greater potency and safety as a therapeutic. The epitope of 11D7 was mapped by competitive binding with well-characterized mAbs, suggesting that it is a Class 4 RBD-binding mAb that binds to the RBD outside the ACE2 binding site. Of note, 11D7 maintains recognition against the B.1.1.529 (Omicron) RBD, as well neutralizing activity. We also provide evidence that this novel mAb may be useful in providing additional synergy to established antibody cocktails, such as Evusheld™ containing the antibodies tixagevimab and cilgavimab, against the Omicron variant. Taken together, 11D7 is a unique mAb that neutralizes SARS-CoV-2 through a mechanism that is not typical among developed therapeutic mAbs and by being produced in ΔXFT Nicotiana benthamiana plants, highlights the potential of plants to be an economic and safety-friendly alternative platform for generating mAbs to address the evolving SARS-CoV-2 crisis.
https://onlinelibrary.wiley.com/doi/10.1111/pbi.13970
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New treatment for COVID-19 is made from plants
February 20, 2023
ASU News
... The treatment could be particularly useful for elderly patients and people with compromised immune systems who are highly vulnerable to SARS-CoV-2 and its emergent variants. The new therapy could also be added to existing therapies for COVID-19, significantly enhancing their protection. Further, using plants to produce therapeutics offers several advantages over conventional methods, including reduced cost, safety and speed of development.
... Because the research describes a different monoclonal antibody mechanism of action, it also advances basic knowledge of how mAbs work against SARS-CoV-2 infection.
The new research has been selected for the cover of the current issue of Plant Biology Journal. ...
Collin Jugler, Haiyan Sun, Katherine Nguyen, Roman Palt, Mitchell Felder, Herta Steinkellner, Qiang Chen
Summary
This study describes a novel, neutralizing monoclonal antibody (mAb), 11D7, discovered by mouse immunization and hybridoma generation, against the parental Wuhan-Hu-1 RBD of SARS-CoV-2. We further developed this mAb into a chimeric human IgG and recombinantly expressed it in plants to produce a mAb with human-like, highly homogenous N-linked glycans that has potential to impart greater potency and safety as a therapeutic. The epitope of 11D7 was mapped by competitive binding with well-characterized mAbs, suggesting that it is a Class 4 RBD-binding mAb that binds to the RBD outside the ACE2 binding site. Of note, 11D7 maintains recognition against the B.1.1.529 (Omicron) RBD, as well neutralizing activity. We also provide evidence that this novel mAb may be useful in providing additional synergy to established antibody cocktails, such as Evusheld™ containing the antibodies tixagevimab and cilgavimab, against the Omicron variant. Taken together, 11D7 is a unique mAb that neutralizes SARS-CoV-2 through a mechanism that is not typical among developed therapeutic mAbs and by being produced in ΔXFT Nicotiana benthamiana plants, highlights the potential of plants to be an economic and safety-friendly alternative platform for generating mAbs to address the evolving SARS-CoV-2 crisis.
https://onlinelibrary.wiley.com/doi/10.1111/pbi.13970
_____________________________________________
New treatment for COVID-19 is made from plants
February 20, 2023
ASU News
... The treatment could be particularly useful for elderly patients and people with compromised immune systems who are highly vulnerable to SARS-CoV-2 and its emergent variants. The new therapy could also be added to existing therapies for COVID-19, significantly enhancing their protection. Further, using plants to produce therapeutics offers several advantages over conventional methods, including reduced cost, safety and speed of development.
... Because the research describes a different monoclonal antibody mechanism of action, it also advances basic knowledge of how mAbs work against SARS-CoV-2 infection.
The new research has been selected for the cover of the current issue of Plant Biology Journal. ...
