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To the Editor,
We previously reported the detection of SARS-CoV-2 in different clinical samples1 . This virus can be detected on different surfaces in a contaminated site2 . Here, we report the stability of SARS-CoV-2 in different environmental conditions. We first determined the stability of SARS-CoV-2 at different temperatures. SARS-CoV-2 in virus transport medium (VTM; final concentration: ∼6.8 log TCID50/mL) was incubated for up to 14 days and then tested for its infectivity (Table A).
The virus is highly stable at 4?C, but sensitive to heat. At 4?C, there was only ∼0.7-log unit reduction of infectious titre on Day 14. With the incubation temperature being increased to 70?C, the time for virus inactivation was reduced to 5 minutes. We further investigated the stability of this virus on different surfaces. In brief, a 5-?L droplet of virus culture (∼7.8 Log unit of TCID50/mL) was pipetted on a surface (Table B; ∼1cm2 per piece) and left at room temperature (22?C; Relative humidity: ∼65%).
The inoculated objects retrieved at desired time points were immediately soaked with 200 ?L of VTM for 30 minutes to elute the virus. No infectious virus could be recovered from printing and tissue papers after a 3-hour incubation, whereas no infectious virus could be detected from treated wood and cloth on Day 2. By contrast, SARS-CoV-2 was more stable on smooth surfaces.
No infectious virus could be detected from treated smooth surfaces on Day 4 (glass and banknote) or Day 7 (stainless steel and plastic). Strikingly, a significant level of infectious virus could still be detected on the outer layer of a surgical mask on Day 7 (∼0.1% of the original inoculum). Interestingly, a biphasic decay of infectious SARS-CoV-2 could be found from samples recovered from these smooth surfaces (Appendix).
Representative negative samples were tested positive by RT-PCR3 (N=39; data not shown), demonstrating that non-infectious viruses could be recovered by the eluents. We also tested the virucidal effects of disinfectants by adding 15 ?L of SARS-CoV-2 culture (∼7.8 Log unit of TCID50/mL) to 135 ?L of various disinfectants at working concentration (Table C). With the exception of a 5-min incubation with hand soap, no infectious virus could be detected after a 5-minute incubation at room temperature.
In addition, we also found that It is made available under a CC-BY-NC-ND 4.0 International license . author/funder, who has granted medRxiv a license to display the preprint in perpetuity. medRxiv preprint doi: https://doi.org/10.1101/2020.03.15.20036673.
The copyright holder for this preprint (which was not peer-reviewed) is the SARS-CoV-2 is extremely stable in a wide-range of pH values at room temperature (pH3-10; Table D) Overall, SARS-CoV-2 can be highly stable in a favourable environment4 , but it is also susceptible to standard disinfection methods.
We previously reported the detection of SARS-CoV-2 in different clinical samples1 . This virus can be detected on different surfaces in a contaminated site2 . Here, we report the stability of SARS-CoV-2 in different environmental conditions. We first determined the stability of SARS-CoV-2 at different temperatures. SARS-CoV-2 in virus transport medium (VTM; final concentration: ∼6.8 log TCID50/mL) was incubated for up to 14 days and then tested for its infectivity (Table A).
The virus is highly stable at 4?C, but sensitive to heat. At 4?C, there was only ∼0.7-log unit reduction of infectious titre on Day 14. With the incubation temperature being increased to 70?C, the time for virus inactivation was reduced to 5 minutes. We further investigated the stability of this virus on different surfaces. In brief, a 5-?L droplet of virus culture (∼7.8 Log unit of TCID50/mL) was pipetted on a surface (Table B; ∼1cm2 per piece) and left at room temperature (22?C; Relative humidity: ∼65%).
The inoculated objects retrieved at desired time points were immediately soaked with 200 ?L of VTM for 30 minutes to elute the virus. No infectious virus could be recovered from printing and tissue papers after a 3-hour incubation, whereas no infectious virus could be detected from treated wood and cloth on Day 2. By contrast, SARS-CoV-2 was more stable on smooth surfaces.
No infectious virus could be detected from treated smooth surfaces on Day 4 (glass and banknote) or Day 7 (stainless steel and plastic). Strikingly, a significant level of infectious virus could still be detected on the outer layer of a surgical mask on Day 7 (∼0.1% of the original inoculum). Interestingly, a biphasic decay of infectious SARS-CoV-2 could be found from samples recovered from these smooth surfaces (Appendix).
Representative negative samples were tested positive by RT-PCR3 (N=39; data not shown), demonstrating that non-infectious viruses could be recovered by the eluents. We also tested the virucidal effects of disinfectants by adding 15 ?L of SARS-CoV-2 culture (∼7.8 Log unit of TCID50/mL) to 135 ?L of various disinfectants at working concentration (Table C). With the exception of a 5-min incubation with hand soap, no infectious virus could be detected after a 5-minute incubation at room temperature.
In addition, we also found that It is made available under a CC-BY-NC-ND 4.0 International license . author/funder, who has granted medRxiv a license to display the preprint in perpetuity. medRxiv preprint doi: https://doi.org/10.1101/2020.03.15.20036673.
The copyright holder for this preprint (which was not peer-reviewed) is the SARS-CoV-2 is extremely stable in a wide-range of pH values at room temperature (pH3-10; Table D) Overall, SARS-CoV-2 can be highly stable in a favourable environment4 , but it is also susceptible to standard disinfection methods.
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