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INSTITUT PASTEUR SEQUENCES THE WHOLE GENOME OF THE WUHAN CORONAVIRUS, 2019-NCOV
Whole sequence of the coronavirus 2019-nCoV, from samples of suspected cases in France.
PRESS RELEASE 2020.01.30
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Friday January 24, 2020. Detection of the virus confirmed in France
On Friday January 24, late in the morning, the Institut Pasteur received samples of three suspected cases (two patients in Paris and one in Bordeaux). "Using the samples taken from these patients, we detected the novel coronavirus," says Sylvie Behillil, Deputy Director of the CNR at the Institut Pasteur.
From Friday January 24, 2020. Viral genome sequenced at the Institut Pasteur
That same Friday evening, scientists launched the process of sequencing the viral genome based on the samples. The CNR prepared the material for sequencing, ready for P2M to begin work immediately the following Monday. The sequencing run was completed by early evening on the Tuesday, and the scientists used data analysis to obtain the sequence of the whole genome in two of the first three confirmed cases in France. "This proves the efficacy of the CNR's process of analysis based on viral sequencing," continues Vincent Enouf.
Thursday January 30, 2020. The Institut Pasteur obtains and shares the whole sequence of the virus
The P2M platform (see inset below) currently performs at an extremely high level; the average time taken to produce sequences ranges from three days (for emergencies) to a maximum of ten days. In this case, it took just three days for the whole sequence to be determined: "We performed data analysis during the night from Tuesday to Wednesday, then corroborated the results on Wednesday with counter analysis," explains Vincent Enouf. "The whole sequence was confirmed in just three days."
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What can we learn from it? "The sequences were identical in all our samples. One member of the couple must have contaminated the other, as the virus is the same." The two full sequences of the virus isolated in two of the first French cases were submitted to the Global Initiative on Sharing All Influenza Data (GISAID) platform,1 which was initially developed to share sequences and monitor the genetic evolution of influenza viruses, a process that is vital to determine the composition of the influenza vaccine. A special "coronavirus" tab has been created so that the scientific community can work together and advance at a quicker pace.
"Around twenty other sequences of the novel coronavirus genome have been obtained worldwide, and if we compare them with ours, we can see that they are all very close; there is not much diversity in the viruses analyzed, which suggests that coronavirus 2019-nCoV did not need to mutate in order to adapt and spread," continues Vincent Enouf.
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Whole sequence of the coronavirus 2019-nCoV, from samples of suspected cases in France.
PRESS RELEASE 2020.01.30
...
Friday January 24, 2020. Detection of the virus confirmed in France
On Friday January 24, late in the morning, the Institut Pasteur received samples of three suspected cases (two patients in Paris and one in Bordeaux). "Using the samples taken from these patients, we detected the novel coronavirus," says Sylvie Behillil, Deputy Director of the CNR at the Institut Pasteur.
From Friday January 24, 2020. Viral genome sequenced at the Institut Pasteur
That same Friday evening, scientists launched the process of sequencing the viral genome based on the samples. The CNR prepared the material for sequencing, ready for P2M to begin work immediately the following Monday. The sequencing run was completed by early evening on the Tuesday, and the scientists used data analysis to obtain the sequence of the whole genome in two of the first three confirmed cases in France. "This proves the efficacy of the CNR's process of analysis based on viral sequencing," continues Vincent Enouf.
Thursday January 30, 2020. The Institut Pasteur obtains and shares the whole sequence of the virus
The P2M platform (see inset below) currently performs at an extremely high level; the average time taken to produce sequences ranges from three days (for emergencies) to a maximum of ten days. In this case, it took just three days for the whole sequence to be determined: "We performed data analysis during the night from Tuesday to Wednesday, then corroborated the results on Wednesday with counter analysis," explains Vincent Enouf. "The whole sequence was confirmed in just three days."
...
What can we learn from it? "The sequences were identical in all our samples. One member of the couple must have contaminated the other, as the virus is the same." The two full sequences of the virus isolated in two of the first French cases were submitted to the Global Initiative on Sharing All Influenza Data (GISAID) platform,1 which was initially developed to share sequences and monitor the genetic evolution of influenza viruses, a process that is vital to determine the composition of the influenza vaccine. A special "coronavirus" tab has been created so that the scientific community can work together and advance at a quicker pace.
"Around twenty other sequences of the novel coronavirus genome have been obtained worldwide, and if we compare them with ours, we can see that they are all very close; there is not much diversity in the viruses analyzed, which suggests that coronavirus 2019-nCoV did not need to mutate in order to adapt and spread," continues Vincent Enouf.
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