tetano
Editor, Senior Moderator
Zhong Nan Da Xue Xue Bao Yi Xue Ban
. 2021 Nov 28;46(11):1203-1211.
doi: 10.11817/j.issn.1672-7347.2021.210221.
Change of gene expression profiles in human cardiomyocytes and macrophages infected with SARS - CoV - 2 and its significance
[Article in English, Chinese]
Yumeng Yang[SUP] 1 [/SUP], Shaowei Wang[SUP] 2 [/SUP], Xinyi Xie[SUP] 3 [/SUP], Junjie Li[SUP] 3 [/SUP], Rongqiang Zhang[SUP] 4 [/SUP]
Affiliations
Abstract
in English, Chinese
Objectives: Coronavirus disease 2019 (COVID-19) is an acute respiratory infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). SARS-CoV-2 can damage the myocardium directly, or activate the immune system, trigger a cytokine storm, and cause inflammatory cells to infiltrate the myocardial tissue and damage the myocardium. This study is based on the sequencing data to analyze the changes in gene expression of cardiomyocytes and macrophages after SARS-CoV-2 infection, and explore the potential effects of SARS-CoV-2 on the heart and immune system.
Methods: The public data set GSE151879 was retrieved. The online software Network Analyst was used to preprocess the data, and the differentially expressed genes (DEGs) [log[SUB]2[/SUB](fold change)>2, adjusted P-value<0.05] screening between the infection group and the control group in cardiomyocytes, human embryonic stem cell-derived cardiomyocytes, and macrophages were screened. Consistent common differentially expressed genes (CCDEGs) with the same expression pattern in cardiomyocytes and macrophages were obtained, and the online analysis software String was used to conduct enrichment analysis of their biological functions and signal pathways. Protein-protein interaction network, transcription factor-gene interaction network, miRNA-gene interaction network and environmental chemical-gene interaction network were established, and Cytoscape 3.72 was used to perform visualization.
Results: After data standardization, the data quality was excellent and it can ensure reliable results. Myocardial cell infection with SARS-CoV-2 and gene expression spectrum were changed significantly, including a total of 484 DEGs in adult cardiomyoblasts, a total of 667 DEGs in macrophages, and a total of 1 483 DEGs in human embryo source of cardiomyopathy. The Stum, mechanosensory transduction mediator homolog (STUM), dehydrogenase/reductase 9 (DHRS9), calcium/calmodulin dependent protein kinase II beta (CAMK2B), claudin 1(CLDN1), C-C motif chemokine ligand 2 (CCL2), TNFAIP3 interacting protein 3 (TNIP3), G protein-coupled receptor 84 (GPR84), and C-X-C motif chemokine ligand 1 (CXCL1) were identical in expression patterns in 3 types of cells. The protein-protein interaction suggested that CAMK2B proteins may play a key role in the antiviral process in 3 types of cells; and silicon dioxide (SiO[SUB]2[/SUB]), benzodiazepine (BaP), nickel (Ni), and estradiol (E[SUB]2[/SUB]) affect anti-SARS-CoV-2 processes of the 3 types of cells.
Conclusions: CAMK2B, CLDN1, CCL2, and DHRS9 genes play important roles in the immune response of cardiomyocytes against SARS-CoV-2. SiO[SUB]2[/SUB], BaP, Ni, E[SUB]2[/SUB] may affect the cell's antiviral process by increasing the toxicity of cardiomyocytes, thereby aggravating SARS-CoV-2 harm to the heart.
Keywords: cardiomyocytes; coronavirus disease 2019; environmental chemicals; immune response; macrophages; severe acute respiratory syndrome coronavirus 2.
. 2021 Nov 28;46(11):1203-1211.
doi: 10.11817/j.issn.1672-7347.2021.210221.
Change of gene expression profiles in human cardiomyocytes and macrophages infected with SARS - CoV - 2 and its significance
[Article in English, Chinese]
Yumeng Yang[SUP] 1 [/SUP], Shaowei Wang[SUP] 2 [/SUP], Xinyi Xie[SUP] 3 [/SUP], Junjie Li[SUP] 3 [/SUP], Rongqiang Zhang[SUP] 4 [/SUP]
Affiliations
- PMID: 34911854
- DOI: 10.11817/j.issn.1672-7347.2021.210221
Abstract
in English, Chinese
Objectives: Coronavirus disease 2019 (COVID-19) is an acute respiratory infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). SARS-CoV-2 can damage the myocardium directly, or activate the immune system, trigger a cytokine storm, and cause inflammatory cells to infiltrate the myocardial tissue and damage the myocardium. This study is based on the sequencing data to analyze the changes in gene expression of cardiomyocytes and macrophages after SARS-CoV-2 infection, and explore the potential effects of SARS-CoV-2 on the heart and immune system.
Methods: The public data set GSE151879 was retrieved. The online software Network Analyst was used to preprocess the data, and the differentially expressed genes (DEGs) [log[SUB]2[/SUB](fold change)>2, adjusted P-value<0.05] screening between the infection group and the control group in cardiomyocytes, human embryonic stem cell-derived cardiomyocytes, and macrophages were screened. Consistent common differentially expressed genes (CCDEGs) with the same expression pattern in cardiomyocytes and macrophages were obtained, and the online analysis software String was used to conduct enrichment analysis of their biological functions and signal pathways. Protein-protein interaction network, transcription factor-gene interaction network, miRNA-gene interaction network and environmental chemical-gene interaction network were established, and Cytoscape 3.72 was used to perform visualization.
Results: After data standardization, the data quality was excellent and it can ensure reliable results. Myocardial cell infection with SARS-CoV-2 and gene expression spectrum were changed significantly, including a total of 484 DEGs in adult cardiomyoblasts, a total of 667 DEGs in macrophages, and a total of 1 483 DEGs in human embryo source of cardiomyopathy. The Stum, mechanosensory transduction mediator homolog (STUM), dehydrogenase/reductase 9 (DHRS9), calcium/calmodulin dependent protein kinase II beta (CAMK2B), claudin 1(CLDN1), C-C motif chemokine ligand 2 (CCL2), TNFAIP3 interacting protein 3 (TNIP3), G protein-coupled receptor 84 (GPR84), and C-X-C motif chemokine ligand 1 (CXCL1) were identical in expression patterns in 3 types of cells. The protein-protein interaction suggested that CAMK2B proteins may play a key role in the antiviral process in 3 types of cells; and silicon dioxide (SiO[SUB]2[/SUB]), benzodiazepine (BaP), nickel (Ni), and estradiol (E[SUB]2[/SUB]) affect anti-SARS-CoV-2 processes of the 3 types of cells.
Conclusions: CAMK2B, CLDN1, CCL2, and DHRS9 genes play important roles in the immune response of cardiomyocytes against SARS-CoV-2. SiO[SUB]2[/SUB], BaP, Ni, E[SUB]2[/SUB] may affect the cell's antiviral process by increasing the toxicity of cardiomyocytes, thereby aggravating SARS-CoV-2 harm to the heart.
Keywords: cardiomyocytes; coronavirus disease 2019; environmental chemicals; immune response; macrophages; severe acute respiratory syndrome coronavirus 2.