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Routine brucellosis surveillance testing has identified brucellosis affected cattle herds in both Park and Sublette Counties. Laboratory testing was conducted at the Wyoming State Veterinary Laboratory (WSVL), Laramie, and results were confirmed at the National Veterinary Services Laboratory, Ames, Iowa. Both herds are located within Wyoming’s Brucellosis Designated Surveillance Area (DSA). With the addition of these herds, WLSB currently has three herds in Park and one herd in Sublette County under quarantine for brucellosis.\Both affected herds have been quarantined following confirmation of test results. No contact herd quarantines are anticipated. The affected herds are currently participating in serial testing to meet the requirements for quarantine release. Testing of affected herds is a cooperative effort between herd owners, private veterinary practitioners, Wyoming Livestock Board (WLSB) personnel, and United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service (APHIS), Veterinary Services (VS) personnel. ...