tetano
Editor, Senior Moderator
World J Clin Cases
. 2020 Dec 26;8(24):6252-6263.
doi: 10.12998/wjcc.v8.i24.6252.
Virus load and virus shedding of SARS-CoV-2 and their impact on patient outcomes
Pei-Fen Chen[SUP] 1 [/SUP], Xia-Xia Yu[SUP] 2 [/SUP], Yi-Peng Liu[SUP] 1 [/SUP], Di Ren[SUP] 3 [/SUP], Min Shen[SUP] 4 [/SUP], Bing-Sheng Huang[SUP] 5 [/SUP], Jun-Ling Gao[SUP] 6 [/SUP], Zheng-Yang Huang[SUP] 2 [/SUP], Ming Wu[SUP] 7 [/SUP], Wei-Yan Wang[SUP] 4 [/SUP], Li Chen[SUP] 4 [/SUP], Xia Shi[SUP] 4 [/SUP], Zhao-Qing Wang[SUP] 4 [/SUP], Ying-Xia Liu[SUP] 4 [/SUP], Lei Liu[SUP] 4 [/SUP], Yong Liu[SUP] 8 [/SUP]
Affiliations
Abstract
Background: Understanding a virus shedding patterns in body fluids/secretions is important to determine the samples to be used for diagnosis and to formulate infection control measures.
Aim: To investigate the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) shedding patterns and its risk factors.
Methods: All laboratory-confirmed coronavirus disease 2019 patients with complete medical records admitted to the Shenzhen Third People's Hospital from January 28, 2020 to March 8, 2020 were included. Among 145 patients (54.5% males; median age, 46.1 years), three (2.1%) died. The bronco-alveolar lavage fluid (BALF) had the highest virus load compared with the other samples. The viral load peaked at admission (3.3 ? 10[SUP]8[/SUP] copies) and sharply decreased 10 d after admission.
Results: The viral load was associated with prolonged intensive care unit (ICU) duration. Patients in the ICU had significantly longer shedding time compared to those in the wards (P < 0.0001). Age > 60 years [hazard ratio (HR) = 0.6; 95% confidence interval (CI): 0.4-0.9] was an independent risk factor for SARS-CoV-2 shedding, while chloroquine (HR = 22.8; 95%CI: 2.3-224.6) was a protective factor.
Conclusion: BALF had the highest SARS-CoV-2 load. Elderly patients had higher virus loads, which was associated with a prolonged ICU stay. Chloroquine was associated with shorter shedding duration and increased the chance of viral negativity.
Keywords: COVID-19; China; Infectious disease; Patient outcome; Viral load; Virus shedding.
. 2020 Dec 26;8(24):6252-6263.
doi: 10.12998/wjcc.v8.i24.6252.
Virus load and virus shedding of SARS-CoV-2 and their impact on patient outcomes
Pei-Fen Chen[SUP] 1 [/SUP], Xia-Xia Yu[SUP] 2 [/SUP], Yi-Peng Liu[SUP] 1 [/SUP], Di Ren[SUP] 3 [/SUP], Min Shen[SUP] 4 [/SUP], Bing-Sheng Huang[SUP] 5 [/SUP], Jun-Ling Gao[SUP] 6 [/SUP], Zheng-Yang Huang[SUP] 2 [/SUP], Ming Wu[SUP] 7 [/SUP], Wei-Yan Wang[SUP] 4 [/SUP], Li Chen[SUP] 4 [/SUP], Xia Shi[SUP] 4 [/SUP], Zhao-Qing Wang[SUP] 4 [/SUP], Ying-Xia Liu[SUP] 4 [/SUP], Lei Liu[SUP] 4 [/SUP], Yong Liu[SUP] 8 [/SUP]
Affiliations
- PMID: 33392306
- PMCID: PMC7760445
- DOI: 10.12998/wjcc.v8.i24.6252
Abstract
Background: Understanding a virus shedding patterns in body fluids/secretions is important to determine the samples to be used for diagnosis and to formulate infection control measures.
Aim: To investigate the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) shedding patterns and its risk factors.
Methods: All laboratory-confirmed coronavirus disease 2019 patients with complete medical records admitted to the Shenzhen Third People's Hospital from January 28, 2020 to March 8, 2020 were included. Among 145 patients (54.5% males; median age, 46.1 years), three (2.1%) died. The bronco-alveolar lavage fluid (BALF) had the highest virus load compared with the other samples. The viral load peaked at admission (3.3 ? 10[SUP]8[/SUP] copies) and sharply decreased 10 d after admission.
Results: The viral load was associated with prolonged intensive care unit (ICU) duration. Patients in the ICU had significantly longer shedding time compared to those in the wards (P < 0.0001). Age > 60 years [hazard ratio (HR) = 0.6; 95% confidence interval (CI): 0.4-0.9] was an independent risk factor for SARS-CoV-2 shedding, while chloroquine (HR = 22.8; 95%CI: 2.3-224.6) was a protective factor.
Conclusion: BALF had the highest SARS-CoV-2 load. Elderly patients had higher virus loads, which was associated with a prolonged ICU stay. Chloroquine was associated with shorter shedding duration and increased the chance of viral negativity.
Keywords: COVID-19; China; Infectious disease; Patient outcome; Viral load; Virus shedding.