tetano
Editor, Senior Moderator
J Immunol. 2016 Apr 6. pii: 1600046. [Epub ahead of print]
[h=1]Whole-Virion Influenza Vaccine Recalls an Early Burst of High-Affinity Memory B Cell Response through TLR Signaling.[/h] Onodera T[SUP]1[/SUP], Hosono A[SUP]2[/SUP], Odagiri T[SUP]3[/SUP], Tashiro M[SUP]3[/SUP], Kaminogawa S[SUP]2[/SUP], Okuno Y[SUP]4[/SUP], Kurosaki T[SUP]5[/SUP], Ato M[SUP]1[/SUP], Kobayashi K[SUP]1[/SUP], Takahashi Y[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Inactivated influenza vaccines have two formulations, whole- and split-virion types; however, how differential formulations impact their booster effects remain unknown. In this study, we demonstrate that whole-virion vaccines recall two waves of Ab responses, early T cell-independent (TI) and late T cell-dependent responses, whereas split-virion vaccines elicit the late T cell-dependent response only. Notably, higher-affinity Abs with improved neutralizing activity are provided from the early TI response, which emphasizes the important contribution of the formulation-dependent response in the protective immunity. Moreover, we show that the early TI response completely requires B cell-intrinsic TLR7 signaling, which can be delivered through viral RNAs within whole-virion vaccine. Thus, our results indicate that TLR agonists in whole-virion type improve recall Ab responses by directly targeting memory B cells, a finding with important implications for vaccine strategies aimed at the prompt recall of high-affinity neutralizing Abs.
Copyright ? 2016 by The American Association of Immunologists, Inc.
PMID: 27053762 [PubMed - as supplied by publisher]
[h=1]Whole-Virion Influenza Vaccine Recalls an Early Burst of High-Affinity Memory B Cell Response through TLR Signaling.[/h] Onodera T[SUP]1[/SUP], Hosono A[SUP]2[/SUP], Odagiri T[SUP]3[/SUP], Tashiro M[SUP]3[/SUP], Kaminogawa S[SUP]2[/SUP], Okuno Y[SUP]4[/SUP], Kurosaki T[SUP]5[/SUP], Ato M[SUP]1[/SUP], Kobayashi K[SUP]1[/SUP], Takahashi Y[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Inactivated influenza vaccines have two formulations, whole- and split-virion types; however, how differential formulations impact their booster effects remain unknown. In this study, we demonstrate that whole-virion vaccines recall two waves of Ab responses, early T cell-independent (TI) and late T cell-dependent responses, whereas split-virion vaccines elicit the late T cell-dependent response only. Notably, higher-affinity Abs with improved neutralizing activity are provided from the early TI response, which emphasizes the important contribution of the formulation-dependent response in the protective immunity. Moreover, we show that the early TI response completely requires B cell-intrinsic TLR7 signaling, which can be delivered through viral RNAs within whole-virion vaccine. Thus, our results indicate that TLR agonists in whole-virion type improve recall Ab responses by directly targeting memory B cells, a finding with important implications for vaccine strategies aimed at the prompt recall of high-affinity neutralizing Abs.
Copyright ? 2016 by The American Association of Immunologists, Inc.
PMID: 27053762 [PubMed - as supplied by publisher]