Shiloh
Editor, Senior Moderator
Source: http://jid.oxfordjournals.org/conte...full?sid=e2e6c39c-1d0b-428a-9c7d-862d4969e3ca
Whole-Genome Sequencing of Measles Virus Genotypes H1 and D8 During Outbreaks of Infection Following the 2010 Olympic Winter Games Reveals Viral Transmission Routes
Jennifer L. Gardy, Monika Naus, Ashraf Amlani, Walter Chung, Hochan Kim, Malcolm Tan, Alberto Severini, Mel Krajden, David Puddicombe, Vanita Sahni, Althea S. Hayden, Reka Gustafson, Bonnie Henry, and Patrick Tang
Correspondence: Jennifer L. Gardy, PhD, BC Centre for Disease Control, 655 W 12th Ave, Vancouver, BC, Canada V5Z 4R4 (jennifer.gardy@bccdc.ca).
Presented in part: 20th Annual International Meeting on Microbial Genomics, Lake Arrowhead, California, 17 September 2014.
Abstract
We used whole-genome sequencing to investigate a dual-genotype outbreak of measles occurring after the XXI Olympic Winter Games in Vancouver, Canada. By sequencing 27 complete genomes from H1 and D8 genotype measles viruses isolated from outbreak cases, we estimated the virus mutation rate, determined that person-to-person transmission is typically associated with 0 mutations between isolates, and established that a single introduction of H1 virus led to the expansion of the outbreak beyond Vancouver. This is the largest measles genomics project to date, revealing novel aspects of measles virus genetics and providing new insights into transmission of this reemerging viral pathogen...
Whole-Genome Sequencing of Measles Virus Genotypes H1 and D8 During Outbreaks of Infection Following the 2010 Olympic Winter Games Reveals Viral Transmission Routes
Jennifer L. Gardy, Monika Naus, Ashraf Amlani, Walter Chung, Hochan Kim, Malcolm Tan, Alberto Severini, Mel Krajden, David Puddicombe, Vanita Sahni, Althea S. Hayden, Reka Gustafson, Bonnie Henry, and Patrick Tang
Correspondence: Jennifer L. Gardy, PhD, BC Centre for Disease Control, 655 W 12th Ave, Vancouver, BC, Canada V5Z 4R4 (jennifer.gardy@bccdc.ca).
Presented in part: 20th Annual International Meeting on Microbial Genomics, Lake Arrowhead, California, 17 September 2014.
Abstract
We used whole-genome sequencing to investigate a dual-genotype outbreak of measles occurring after the XXI Olympic Winter Games in Vancouver, Canada. By sequencing 27 complete genomes from H1 and D8 genotype measles viruses isolated from outbreak cases, we estimated the virus mutation rate, determined that person-to-person transmission is typically associated with 0 mutations between isolates, and established that a single introduction of H1 virus led to the expansion of the outbreak beyond Vancouver. This is the largest measles genomics project to date, revealing novel aspects of measles virus genetics and providing new insights into transmission of this reemerging viral pathogen...