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WHO: Availability of new recombinant H5N1 vaccine virus

L

Laidback Al

Well-known member
Availability of new recombinant H5N1 vaccine virus
21 December 2006
The WHO Global Influenza Programme is continuing to monitor the antigenic and genetic evolution of circulating H5N1 viruses and their human isolates. In August 2006, WHO reported<SUP>1</SUP> that analysis of newly isolated viruses collected from both animals and humans indicated that the H5 haemagglutinin (HA) genes had become genetically distinguishable from the H5N1 viruses that had previously been selected for vaccine development. Furthermore, there was also evidence of antigenic variation among the recent viruses. Since then, WHO Collaborating Centres (WHO CCs) and H5 Reference Laboratories have been developing several new recombinant H5N1 vaccine viruses that are representative of this newly discovered genetic sub-group of clade 2 viruses.
The WHO CC in the Centers for Disease Control and Prevention (CDC), Atlanta USA and the China Centers for Disease Control, Beijing China have together developed a new recombinant H5N1 virus from A/Anhui/1/2005 selected from clade 2, sub-clade 3. The recombinant vaccine virus is available for distribution, under a Material Transfer Agreement (MTA). The sequences of haemagglutinin (HA) and neuraminidase (NA) of the new H5N1 recombinant vaccine virus can be found on the public web site of Los Alamos National Laboratory database together with all other WHO selected and developed influenza vaccine viruses for both seasonal and H5N1 influenza.
Institutions, companies and others interested in pandemic vaccine development, who wish to receive this recombinant vaccine virus should contact either the WHO Global Influenza Programme at whoinfluenza@who.int or WHO CC CDC at the address below:
WHO Collaborating Center for Surveillance, Epidemiology and Control of Influenza, Centers for Disease Control and Prevention, 1600 Clifton Road, Mail stop G16, Atlanta, GA 30333, USA (fax: +1 404 639 23 34; e-mail: rdonis@cdc.gov).
Review of the needs and the actual development of H5N1 vaccine viruses representative of circulating strains in the world are ongoing in WHO CCs and H5 Reference Laboratories. The WHO Global Influenza Programme will continue to select, verify and develop new H5N1 candidate vaccine viruses from genetically and/or antigenically different groups of circulating viruses for the purposes of vaccine research and development.
-------------------------
<SUP>1</SUP> Weekly Epidemiological Report 2006; 81(34/35):328?330. Antigenic and genetic characteristics of H5N1 viruses and candidate H5N1 vaccine viruses.


http://www.who.int/csr/disease/avian_influenza/guidelines/h5n1vaccinevirus/en/index.html
 
Re: WHO: Availability of new recombinant H5N1 vaccine virus

Does anyone have the neuraminidase aa sequence for this:
(A/Anhui/1/2005) virus?

I have searched the databases and do not find NA.
Many thanks for your kind reply!
Gene
 
Re: WHO: Availability of new recombinant H5N1 vaccine virus

I can't help with the sequence, but I wanted to welcome you to the forum.

Welcome aboard! :)

MHSC
 
Re: WHO: Availability of new recombinant H5N1 vaccine virus

Thanks for the warm welcome. Now that I know this forum is
FluTrackers and not Flut Rackers, it's much more interesting to me. :smartass:
~g
 
Re: WHO: Availability of new recombinant H5N1 vaccine virus

Gene - Welcome. Someone is looking that up for you.


S.
 
Re: WHO: Availability of new recombinant H5N1 vaccine virus

Ahhhh, bless you, Florida1.
I've bugged Ruben enough already.
~g
 
Re: WHO: Availability of new recombinant H5N1 vaccine virus

GenePalmer said:
Does anyone have the neuraminidase aa sequence for this:
(A/Anhui/1/2005) virus?

I have searched the databases and do not find NA.
Many thanks for your kind reply!
Gene
Click to expand...
Both at Los-Alamos & at NCBI, only the HA sequence is available...

You probably need a WHO's proxy connexion to obtain it from los alamos.

Must be somewhere in the WHO's secret database or in the local China National Influenza Center, IVDC, China CDC, 100 Yingxin jie Xuanwu Qu, Beijing, Beijing 100052, laboratory.

Anyone else got better source ?
 
Re: WHO: Availability of new recombinant H5N1 vaccine virus

A couple of people that may know should log on sometime tonight.
 
Last edited:
Re: WHO: Availability of new recombinant H5N1 vaccine virus

The only publicly available 2005 NA sequences at Los Alamos Flu Database are...

<table class="resultstable" border="0" cellpadding="4" cellspacing="0" width="100%"><tbody><tr bgcolor="white"> <td> ISDN122818 </td> <td> A/Cambodia/JP52a/2005 </td> <td> NA (6) </td> <td> 1365 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="#a7c4ef"> <td align="left"> <input name="checkbox" value="129659" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> AB239126 </td> <td> A/Hanoi/30408/2005 </td> <td> NA (6) </td> <td> 1417 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="white"> <td align="left"> <input name="checkbox" value="130375" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> ISDN130375 </td> <td> A/Indonesia/160H/2005 </td> <td> NA (6) </td> <td> 1362 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="#a7c4ef"> <td align="left"> <input name="checkbox" value="130367" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> ISDN130367 </td> <td> A/Indonesia/175H/2005 </td> <td> NA (6) </td> <td> 1361 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="white"> <td align="left"> <input name="checkbox" value="130914" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> ISDN130914 </td> <td> A/Indonesia/195H/2005 </td> <td> NA (6) </td> <td> 1338 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="#a7c4ef"> <td align="left"> <input name="checkbox" value="132408" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> ISDN132408 </td> <td> A/Indonesia/239H/2005 </td> <td> NA (6) </td> <td> 1327 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="white"> <td align="left"> <input name="checkbox" value="132416" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> ISDN132416 </td> <td> A/Indonesia/245H/2005 </td> <td> NA (6) </td> <td> 1361 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="#a7c4ef"> <td align="left"> <input name="checkbox" value="125875" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> ISDN125875 </td> <td> A/Indonesia/5/05 </td> <td> NA (6) </td> <td> 1328 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="white"> <td align="left"> <input name="checkbox" value="125784" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> ISDN125784 </td> <td> A/Indonesia/5/2005 </td> <td> NA (6) </td> <td> 1388 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="#a7c4ef"> <td align="left"> <input name="checkbox" value="128027" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> ISDN128027 </td> <td> A/Indonesia/6/2005 </td> <td> NA (6) </td> <td> 1363 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="white"> <td align="left"> <input name="checkbox" value="129528" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> ISDN129528 </td> <td> A/Indonesia/7/2005 </td> <td> NA (6) </td> <td> 1381 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="#a7c4ef"> <td align="left"> <input name="checkbox" value="137810" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> ISDN137810 </td> <td> A/Indonesia/CDC184/05 </td> <td> NA (6) </td> <td> 1331 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="white"> <td align="left"> <input name="checkbox" value="207322" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> CY014237 </td> <td> A/Indonesia/CDC184/2005 </td> <td> NA (6) </td> <td> 1331 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="#a7c4ef"> <td align="left"> <input name="checkbox" value="137813" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> ISDN137813 </td> <td> A/Indonesia/CDC194p/05 </td> <td> NA (6) </td> <td> 1331 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="white"> <td align="left"> <input name="checkbox" value="207325" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> CY014240 </td> <td> A/Indonesia/CDC194P/2005 </td> <td> NA (6) </td> <td> 1331 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="#a7c4ef"> <td align="left"> <input name="checkbox" value="137811" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> ISDN137811 </td> <td> A/Indonesia/CDC287e/05 </td> <td> NA (6) </td> <td> 1331 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="white"> <td align="left"> <input name="checkbox" value="207323" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> CY014238 </td> <td> A/Indonesia/CDC287E/2005 </td> <td> NA (6) </td> <td> 1331 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="#a7c4ef"> <td align="left"> <input name="checkbox" value="137800" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> ISDN137800 </td> <td> A/Indonesia/CDC292n/05 </td> <td> NA (6) </td> <td> 1331 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="white"> <td align="left"> <input name="checkbox" value="207312" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> CY014227 </td> <td> A/Indonesia/CDC292N/2005 </td> <td> NA (6) </td> <td> 1331 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="#a7c4ef"> <td align="left"> <input name="checkbox" value="137801" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> ISDN137801 </td> <td> A/Indonesia/CDC292t/05 </td> <td> NA (6) </td> <td> 1331 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="white"> <td align="left"> <input name="checkbox" value="207313" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> CY014228 </td> <td> A/Indonesia/CDC292T/2005 </td> <td> NA (6) </td> <td> 1331 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="#a7c4ef"> <td align="left"> <input name="checkbox" value="129584" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> ISDN129584 </td> <td> A/Indonesia/CDC7/2005 </td> <td> NA (6) </td> <td> 1331 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="white"> <td align="left"> <input name="checkbox" value="207263" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> CY014179 </td> <td> A/Indonesia/CDC7/2005 </td> <td> NA (6) </td> <td> 1331 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="#a7c4ef"> <td align="left"> <input name="checkbox" value="133442" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> DQ360836 </td> <td> A/Thailand/676/2005 </td> <td> NA (6) </td> <td> 1374 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="white"> <td align="left"> <input name="checkbox" value="216322" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> DQ885620 </td> <td> A/Thailand/NA60/2005 </td> <td> NA (6) </td> <td> 1350 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="#a7c4ef"> <td align="left"> <input name="checkbox" value="136483" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> DQ372593 </td> <td> A/Thailand/NK165/2005 </td> <td> NA (6) </td> <td> 1372 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="white"> <td align="left"> <input name="checkbox" value="216313" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> DQ885611 </td> <td> A/Thailand/NKFENA/2005 </td> <td> NA (6) </td> <td> 1336 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="#a7c4ef"> <td align="left"> <input name="checkbox" value="216315" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> DQ885613 </td> <td> A/Thailand/NKNPNA/2005 </td> <td> NA (6) </td> <td> 1336 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="white"> <td align="left"> <input name="checkbox" value="216317" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> DQ885615 </td> <td> A/Thailand/RPFENA/2005 </td> <td> NA (6) </td> <td> 1339 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="#a7c4ef"> <td align="left"> <input name="checkbox" value="216319" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> DQ885617 </td> <td> A/Thailand/RPNPNA/2005 </td> <td> NA (6) </td> <td> 1330 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="white"> <td align="left"> <input name="checkbox" value="132478" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> DQ094290 </td> <td> A/Viet Nam/BL-014/2005 </td> <td> NA (6) </td> <td> 1350 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="#a7c4ef"> <td align="left"> <input name="checkbox" value="132479" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> DQ094291 </td> <td> A/Viet Nam/DT-036/2005 </td> <td> NA (6) </td> <td> 1350 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="white"> <td align="left"> <input name="checkbox" value="132480" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> DQ094292 </td> <td> A/Viet Nam/HG-207/2005 </td> <td> NA (6) </td> <td> 1350 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="#a7c4ef"> <td align="left"> <input name="checkbox" value="119679" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> ISDN119679 </td> <td> A/Viet Nam/HN30408/2005 </td> <td> NA (6) </td> <td> 1350 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="white"> <td align="left"> <input name="checkbox" value="117783" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> ISDN117783 </td> <td> A/Viet Nam/JP14/2005 </td> <td> NA (6) </td> <td> 1350 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="#a7c4ef"> <td align="left"> <input name="checkbox" value="117782" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> ISDN117782 </td> <td> A/Viet Nam/JP4207/2005 </td> <td> NA (6) </td> <td> 1350 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="white"> <td align="left"> <input name="checkbox" value="141334" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> DQ493075 </td> <td> A/Vietnam/CL105/2005 </td> <td> NA (6) </td> <td> 1350 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="#a7c4ef"> <td align="left"> <input name="checkbox" value="141335" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> DQ493076 </td> <td> A/Vietnam/CL115/2005 </td> <td> NA (6) </td> <td> 1350 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="white"> <td align="left"> <input name="checkbox" value="136822" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> DQ250163 </td> <td> A/Vietnam/CL115/2005 </td> <td> NA (6) </td> <td> 1350 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="#a7c4ef"> <td align="left"> <input name="checkbox" value="136823" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> DQ250164 </td> <td> A/Vietnam/CL119/2005 </td> <td> NA (6) </td> <td> 1327 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="white"> <td align="left"> <input name="checkbox" value="141336" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> DQ493077 </td> <td> A/Vietnam/CL119/2005 </td> <td> NA (6) </td> <td> 1326 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="#a7c4ef"> <td align="left"> <input name="checkbox" value="141337" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> DQ493078 </td> <td> A/Vietnam/CL2009/2005 </td> <td> NA (6) </td> <td> 1332 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="white"> <td align="left"> <input name="checkbox" value="136824" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> DQ250165 </td> <td> A/Vietnam/CL2009/2005 </td> <td> NA (6) </td> <td> 1333 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td> </tr> <tr bgcolor="#a7c4ef"> <td align="left"> <input name="checkbox" value="218104" onclick="setAll(this.checked)" type="checkbox"> </td> <td class="displaytablerow" align="left">
</td> <td> DQ535726 </td> <td> A/Vietnam/PEV16T/2005 </td> <td> NA (6) </td> <td> 1350 </td> <td> 2005 </td> <td> H5N1 </td> <td>
</td></tr></tbody></table>
 
Re: WHO: Availability of new recombinant H5N1 vaccine virus

GenePalmer said:
Does anyone have the neuraminidase aa sequence for this:
(A/Anhui/1/2005) virus?

I have searched the databases and do not find NA.
Many thanks for your kind reply!
Gene
Click to expand...

All of the recent human H5N1 are the Fujian strain (Clade 2 subclade 3)

http://www.recombinomics.com/phylo/HA_Vaccine.html

and the only public human NA Fujian sequence is

http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=108946272

(although there are many bird Fujian NA sequences from 2005 and 2006)
 
Re: WHO: Availability of new recombinant H5N1 vaccine virus

GenePalmer said:
Does anyone have the neuraminidase aa sequence for this:
(A/Anhui/1/2005) virus?

I have searched the databases and do not find NA.
Many thanks for your kind reply!
Gene
Click to expand...

This is a close as you will get for a public human sequence

MNPNQKIITIGSICMVIGIVSLMLQIGNMISIWVSHSIQTGNQH
QAEPIRNTNFLTENAVASVTLAGNSSLCPVRGWAVHSKDNSIRIGSKGDVFVIREPFI
SCSHLECRTFFLTQGALLNDKHSNGTVKDRSPHRTLMSCPVGEAPSPYNSRFESVAWS
ASACHDGTSWLTIGISGPDNGAVAVLKYNGIITDTIKSWRNNILRTQESECACVNGSC
FTVMTDGPSNGQASYKIFKMEKGKVVKSVELNAPNYHYEECSCYPDAGEITCVCRDNW
HGSNRPWVSFNQNLEYQIGYICSGVFGDNPRPNDGTGSCGPVSPNGAYGIKGFSFKYG
NGVWIGRTKSTNSRSGFEMIWDPNGWTETDSNFSVKQDIVAITDWSGYSGSFVQHPEL
TGLDCIRPCFWVELIRGRPKESTIWTSGSSISFCGVNSDTVGWSWPDGAELPFTIDK
 
Re: WHO: Availability of new recombinant H5N1 vaccine virus

here the NA of my two closest matches in HA

Code: 
[FONT=Fixedsys]> A/chicken/Guiyang/3721/2005 (H5N1)
---------------------------------------------------------------------------ATTGGGAACATGATCTCAATATGGGTCAGTCATTCAATTCAAACAGGGAATCAACACCAAGCTGAACCAATCAGAAATGCTAATTTTCTTACTGAGAACGCTGTGGCTTCAGTAACATTAGCGGGCAATTCATCTCTTTGCCCCGTTAGAGGATGGGCTGTACACAGTAAAGACAACAGTATAAGGATTGGTTCCAAGGGGGATGTGTTTGTTATTAGAGAGCCGTTCATCTCATGCTCCCACTTGGAATGCAGAACTTTCTTTTTGACTCAGGGAGCCTTACTGAATGACAAGCACTCCAATGGGACTGTCAAAGACAGAAGCCCTCACAGAACATTAATGAGTTGTCCTGTGGGTGAGGCTCCCTCCCCTTATAACTCAAGGTTTGAGTCTGTTGCTTGGTCAGCAAGTGCTTGCCATGATGGCACCAGTTGGTTGACAATTGGAATTTCTGGCCCAGACAATGGGGCTGTGGCTGTATTGAAATACAATGGCATAATAACAGACACCATCAAGAGTTGGAGGAACAACATACTGAGAACTCAAGAGTCTGAATGTGCATGTGTAAATGGCTCTTGCTTTACTGTAATGACTGATGGACCAAGTAATGGGCAGGCATCATATAAGATCTTCAAAATGGAAAAAGGGAAAGTGGTCAAATCAGTCGAATTGAATGCTCCTAATTATCACTATGAGGAATGCTCCTGTTATCCTGATGCTGGCGAAATCACATGTGTGTGCAGGGATAATTGGCATGGCTCAAATAGGCCATGGGTATCTTTCAATCAGAATTTGGAGTATCAAATAGGATATATATGCAGTGGAGTTTTCGGAGACAATCCACGCCCCAATGATGGAACAGGTAGTTGTGGTCCAGTGTCCCCTAACGGGGCATATGGGATAAAAGGGTTTTCATTTAAATACGGCAATGGTGTTTGGATCGGAAGAACCAAAAGCACTAATTCCAGGAGCGGCTTTGAAATGATTTGGGATCCAAATGGGTGGACTGAAACGGACAGTAACTTTTCGGTGAAACAAGATATAGTAGCAATAACTGATTGGTCAGGATATAGCGGGAGTTTTGTCCAGCATCCAGAACTGACAGGATTAGATTGCATAAGACCTTGCTTCTGGGTTGAGTTAATCAGAGGGCGGCCCAAAGAGAGCACAATTTGGACTAGTGGGAGCAGCATATCCTTTTGTGGTGTAAATAGTGACACTGTGAGTTGGTCTTGGCCAGACGGTGCTGAGTTGCCATTCACCATTGACAAGTAG
-------------------------IGNMISIWVSHSIQTGNQHQAEPIRNANFLTENAVASVTLAGNSSLCPVRGWAVHSKDNSIRIGSKGDVFVIREPFISCSHLECRTFFLTQGALLNDKHSNGTVKDRSPHRTLMSCPVGEAPSPYNSRFESVAWSASACHDGTSWLTIGISGPDNGAVAVLKYNGIITDTIKSWRNNILRTQESECACVNGSCFTVMTDGPSNGQASYKIFKMEKGKVVKSVELNAPNYHYEECSCYPDAGEITCVCRDNWHGSNRPWVSFNQNLEYQIGYICSGVFGDNPRPNDGTGSCGPVSPNGAYGIKGFSFKYGNGVWIGRTKSTNSRSGFEMIWDPNGWTETDSNFSVKQDIVAITDWSGYSGSFVQHPELTGLDCIRPCFWVELIRGRPKESTIWTSGSSISFCGVNSDTVSWSWPDGAELPFTIDK}
> A/duck/Guiyang/3996/2005 (H5N1)
ATGAATCCAAATCAGAAGATAATAACCATTGGGTCAATCTGTATGGTAATTGGAATAGTTAGCTTAATGTTACAAATTGGGAACATGATCTCAATATGGGTCAGTCATTCAATTCAAACAGGGAATCAACACCAAGCTGAACCAATCAGAAATGCTAATTTTCTTACTGAGAACGCTGTGACTTCAGTAACATTAGCGGGCAATTCATCTCTTTGCCCCGTTAGAGGATGGGCTGTACACAGTAAAGACAACAGTATAAGGATTGGTTCCAAGGGGGATGTGTTTGTTATTAGAGAGCCGTTCATCTCATGCTCCCACTTGGAATGCAGAACTTTCTTTTTGACTCAGGGAGCCTTACTGAATGACAAGCACTCCAATGGGACTGTCAAAGACAGAAGCCCTCACAGAACATTAATGAGTTGTCCTGTGGGTGAGGCTCCCTCCCCTTATAACTCAAGGTTTGAGTCTGTTGCTTGGTCAGCAAGTGCTTGCCATGATGGCACCAGTTGGTTGACAATTGGAATTTCTGGCCCAGACAATGGGGCTGTGGCTGTATTGAAATACAATGGCATAATAACAGACACCATCAAGAGTTGGAGGAACAACATACTGAGAACTCAAGAGTCTGAATGTGCATGTGTAAATGGCTCTTGCTTTACTGTAATGACTGATGGACCAAGTAATGGGCAGGCATCATATAAGATCTTCAAAATGGAAAAAGGGAAAGTGGTCAAATCAGTCGAATTGAATGCTCCTAATTATCACTATGAGGAATGCTCCTGTTATCCTGATGCTGGCGAAATCACATGTGTGTGCAGGGATAATTGGCATGGCTCAAATAGGCCATGGGTATCTTTCAATCAGAATTTGGAGTATCAAATAGGATATATATGCAGTGGAGTTTTCGGAGACAATCCACGCCCCAATGATGGAACAGGTAGTTGTGGTCCAGTGTCCCCTAACGGGGCATATGGGATAAAAGGGTTTTCATTTAAATACGGCAATGGTGTTTGGATCGGAAGAACCAAAAGCACTAATTCCAGGAGCGGCTTTGAAATGATTTGGGATCCAAATGGGTGGACTGAAACGGACAGTAACTTTTCGGTGAAACAAGATATAGTAGCAATAACTGATTGGTCAGGATATAGCGGGAGTTTTGTCCAGCATCCAGAACTGACAGGATTGGATTGCATAAGACCTTGCTTCTGGATTGAGTTAATCAGAGGGCGGCCCAAAGAGAGCACAATTTGGACTAGTGGGAGCAGCATATCCTTTTGTGGTGTAAATAGTGACACTGTGAGTTGGTCTTGGCCAGACGGTGCTGAGTTGCCATTCACCATTGACAAGTAG
MNPNQKIITIGSICMVIGIVSLMLQIGNMISIWVSHSIQTGNQHQAEPIRNANFLTENAVTSVTLAGNSSLCPVRGWAVHSKDNSIRIGSKGDVFVIREPFISCSHLECRTFFLTQGALLNDKHSNGTVKDRSPHRTLMSCPVGEAPSPYNSRFESVAWSASACHDGTSWLTIGISGPDNGAVAVLKYNGIITDTIKSWRNNILRTQESECACVNGSCFTVMTDGPSNGQASYKIFKMEKGKVVKSVELNAPNYHYEECSCYPDAGEITCVCRDNWHGSNRPWVSFNQNLEYQIGYICSGVFGDNPRPNDGTGSCGPVSPNGAYGIKGFSFKYGNGVWIGRTKSTNSRSGFEMIWDPNGWTETDSNFSVKQDIVAITDWSGYSGSFVQHPELTGLDCIRPCFWIELIRGRPKESTIWTSGSSISFCGVNSDTVSWSWPDGAELPFTIDK}[/FONT]


http://flu.lanl.gov/search/view_record.html?accession=EF124237&database=fluA
http://flu.lanl.gov/search/view_record.html?accession=EF124303&database=fluA



also:
http://flu.lanl.gov/search/view_record.html?accession=ISDN138782&database=fluA
http://flu.lanl.gov/search/view_record.html?accession=EF124241&database=fluA
http://flu.lanl.gov/search/view_record.html?accession=EF124238&database=fluA
 
Re: WHO: Availability of new recombinant H5N1 vaccine virus

GenePalmer said:
Does anyone have the neuraminidase aa sequence for this:
(A/Anhui/1/2005) virus?

I have searched the databases and do not find NA.
Many thanks for your kind reply!
Gene
Click to expand...

Full sequence of human isolate from Guangdong was just released. It is also the Fujian strain and the NA sequence is

MNPNQKIITIGSICMVIGIVSLMLQIGNMVSLWVSHSIQTGNQH
QVEPISNTNFLTEKAVASVTLAGNSSLCPIRGWAVHSKDNSIRIGSKGDVFVIREPFI
SCSHLECRTFFLTQGALLNDKHSNGTVKDRSPHRTLMSCPVGEAPSPYNSRFESVAWS
ASACHDGTSWLTIGISGPDNGAVAVLKYNGMITDTIKSWRNNILRTQESECVCVNGSC
FTVMTDGPSNGQASYKIFKMEKGKVVKSVELDAPNYHYEECSCYPDAGEITCVCRDNW
HGSNRPWVSFNQNLEYQIGYICSGVFGDNPRPNDGTGSCGPVSPNGAYGVKGFSFKYG
NGVWIGRTKSPSSRSGFEMIWDPNGWTETDSSFSVKQDIVAITDWSGYSGSFVQHPEL
TGLDCIRPCFWVELIRGRPKESTIWTSGSSISFCGVNSDTVSWSWPDGAELPFTIDK
 
Re: WHO: Availability of new recombinant H5N1 vaccine virus

Thank you all for your efforts and kind replies. I posted this query after finding
much the same results posted here from the various databases. Specifically
I need the NA amino acid sequence for A/Anhui/1/2005 (clade 2.3 human isolate)
since the CDC has recently announced the availability of this strain as an RG
reassortant. With permit and MTA in place to acquire this virus, we need the
sequence to develop constructs to express in plants for vaccine development.

~gene
 
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