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Wastewater samples CANNOT be used for genome assembly - Virological.

Mary Wilson

Well-known member
March 1, 2023, 12:19pm

Author: Alejandro Gener, PhD

Abstract

Wastewater sequencing is providing important insights enabling early pathogen detection (e.g., emergent SARS-CoV-2 strains/variants) and allowing public health authorities to anticipate community levels of resistance and to predict treatment susceptibility or vaccine efficacy. However, wastewater sequencing should be viewed distinctly from efforts to assemble genomes from individuals most often performed as part of baseline surveillance. It is helpful to include both assembly data and read data to enable trouble-shooting and verification of SARS-CoV-2 study claims. These practices have broad implications for all pathogens currently being monitored with wastewater and baseline surveillance.

https://virological.org/t/wastewater-samples-cannot-be-used-for-genome-assembly/921#post_7
 
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