tetano
Editor, Senior Moderator
The tree shrew provides a useful alternative model for the study of influenza H1N1 virus
Zi-feng Yang, Jin Zhao, Yu-tong Zhu, Yu-tao Wang, Rong Liu, Sui-shan Zhao, Run-feng Li, Chun-guang Yang, Ji-qiang Li and Nan-shan Zhong
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Virology Journal 2013, 10:111 doi:10.1186/1743-422X-10-111
Published: 10 April 2013
Abstract (provisional)
Background
The influenza pandemics have resulted in significant morbidity and mortality worldwide. Animal models are useful in the study of influenza virus pathogenesis. Because of various limitations in current laboratory animal models, it is essential to develop new alternative animal models for influenza virus research aimed at understanding the viral and host factors that contribute to virus infection in human.
Method
We investigated the replicative efficiency of influenza H1N1 virus (classic strain (Influenza A/PR/8/34), seasonal influenza isolate (A/Guangzhou/GIRD/02/09) and swine-origin human influenza virus (A/Guangzhou/GIRD/07/09)) at Day1,2,4,6 and 9 p.i. using TCID50 and qPCR assay in tree shrew model. Body temperature was monitored in the morning and evening for 3 days before infection and for 14 days. Seroconversion was detected by determining the neutralizing antibody titers against the challenge viruses in the pre- and exposure serum samples collected before infection and at 14 days p.i., respectively. Lungs and tracheas of tree shews were collected at day 14 post p.i. for histopathological analysis. Lectinhistochemistry analysis was conducted to identify the distribution of SAalpha2,3 Gal and SAalpha2,6 Gal receptors in the lung and trachea.
Results
The infected tree shrew displayed mild or moderate systemic and respiratory symptoms and pathological changes in respiratory tracts. The human H1N1 influenza virus may replicate in the upper respiratory tract of tree shrews. Analysis of the receptors distribution in the respiratory tract of tree shrews by lectinhistochemistry showed that sialic acid (SA)alpha2,6-Gal receptors were widely distributed in the trachea and nasal mucosa, whereas (SA)alpha2,3-Gal receptor was the main receptor in the lung tissue.
Conclusions
Based on these findings, tree shrew seemed to mimic well influenza virus infection in humans. We propose that tree shrews could be a useful alternative mammalian model to study pathogenesis of influenza H1N1 virus.
full article
http://www.virologyj.com/content/pdf/1743-422X-10-111.pdf
Zi-feng Yang, Jin Zhao, Yu-tong Zhu, Yu-tao Wang, Rong Liu, Sui-shan Zhao, Run-feng Li, Chun-guang Yang, Ji-qiang Li and Nan-shan Zhong
For all author emails, please log on.
Virology Journal 2013, 10:111 doi:10.1186/1743-422X-10-111
Published: 10 April 2013
Abstract (provisional)
Background
The influenza pandemics have resulted in significant morbidity and mortality worldwide. Animal models are useful in the study of influenza virus pathogenesis. Because of various limitations in current laboratory animal models, it is essential to develop new alternative animal models for influenza virus research aimed at understanding the viral and host factors that contribute to virus infection in human.
Method
We investigated the replicative efficiency of influenza H1N1 virus (classic strain (Influenza A/PR/8/34), seasonal influenza isolate (A/Guangzhou/GIRD/02/09) and swine-origin human influenza virus (A/Guangzhou/GIRD/07/09)) at Day1,2,4,6 and 9 p.i. using TCID50 and qPCR assay in tree shrew model. Body temperature was monitored in the morning and evening for 3 days before infection and for 14 days. Seroconversion was detected by determining the neutralizing antibody titers against the challenge viruses in the pre- and exposure serum samples collected before infection and at 14 days p.i., respectively. Lungs and tracheas of tree shews were collected at day 14 post p.i. for histopathological analysis. Lectinhistochemistry analysis was conducted to identify the distribution of SAalpha2,3 Gal and SAalpha2,6 Gal receptors in the lung and trachea.
Results
The infected tree shrew displayed mild or moderate systemic and respiratory symptoms and pathological changes in respiratory tracts. The human H1N1 influenza virus may replicate in the upper respiratory tract of tree shrews. Analysis of the receptors distribution in the respiratory tract of tree shrews by lectinhistochemistry showed that sialic acid (SA)alpha2,6-Gal receptors were widely distributed in the trachea and nasal mucosa, whereas (SA)alpha2,3-Gal receptor was the main receptor in the lung tissue.
Conclusions
Based on these findings, tree shrew seemed to mimic well influenza virus infection in humans. We propose that tree shrews could be a useful alternative mammalian model to study pathogenesis of influenza H1N1 virus.
full article
http://www.virologyj.com/content/pdf/1743-422X-10-111.pdf