tetano
Editor, Senior Moderator
Induction of cross-protection against influenza A virus by DNA prime-intranasal protein boost strategy based on nucleoprotein
Jian Luo, Dan Zheng, Wenjie Zhang, Fang Fang, Hanzhong Wang, Ying Sun, Yahong Ding, Chengfei Xu, Quanjiao Chen, Hongbo Zhang, Ding Huang, Bing Sun and Ze Chen
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Virology Journal 2012, 9:286 doi:10.1186/1743-422X-9-286
Published: 23 November 2012
Abstract (provisional)
Background
The highly conserved nucleoprotein (NP) is an internal protein of influenza virus and is capable of inducing cross-protective immunity against different influenza A viruses, making it a main target of universal influenza vaccine. In current study, we characterized the immune response induced by DNA prime-intranasal protein boost strategy based on NP (A/PR/8/34, H1N1) in mouse model, and evaluated its protection ability against a lethal dose challenge of influenza virus.
Results
The intranasal boost with recombinant NP (rNP) protein could effectively enhance the pre-immune response induced by the NP DNA vaccine in mice. Compared to the vaccination with NP DNA or rNP protein alone, the prime-boost strategy increased the level of NP specific serum antibody, enhanced the T cell immune response, and relatively induced more mucosal IgA antibody. The overall immune response induced by this heterologous prime-boost regimen was Th-1-biased. Furthermore, the immune response in mice induced by this strategy provided not only protection against the homologous virus but also cross-protection against a heterosubtypic H9N2 strain.
Conclusions
The NP DNA prime-intranasal protein boost strategy may provide an effective strategy for universal influenza vaccine development.
http://www.virologyj.com/content/pdf/1743-422X-9-286.pdf
Jian Luo, Dan Zheng, Wenjie Zhang, Fang Fang, Hanzhong Wang, Ying Sun, Yahong Ding, Chengfei Xu, Quanjiao Chen, Hongbo Zhang, Ding Huang, Bing Sun and Ze Chen
For all author emails, please log on.
Virology Journal 2012, 9:286 doi:10.1186/1743-422X-9-286
Published: 23 November 2012
Abstract (provisional)
Background
The highly conserved nucleoprotein (NP) is an internal protein of influenza virus and is capable of inducing cross-protective immunity against different influenza A viruses, making it a main target of universal influenza vaccine. In current study, we characterized the immune response induced by DNA prime-intranasal protein boost strategy based on NP (A/PR/8/34, H1N1) in mouse model, and evaluated its protection ability against a lethal dose challenge of influenza virus.
Results
The intranasal boost with recombinant NP (rNP) protein could effectively enhance the pre-immune response induced by the NP DNA vaccine in mice. Compared to the vaccination with NP DNA or rNP protein alone, the prime-boost strategy increased the level of NP specific serum antibody, enhanced the T cell immune response, and relatively induced more mucosal IgA antibody. The overall immune response induced by this heterologous prime-boost regimen was Th-1-biased. Furthermore, the immune response in mice induced by this strategy provided not only protection against the homologous virus but also cross-protection against a heterosubtypic H9N2 strain.
Conclusions
The NP DNA prime-intranasal protein boost strategy may provide an effective strategy for universal influenza vaccine development.
http://www.virologyj.com/content/pdf/1743-422X-9-286.pdf