tetano
Editor, Senior Moderator
Inactivation of the novel avian influenza A (H7N9) virus under physical conditions or chemical agents treatment
Shumei Zou, Junfeng Guo, Rongbao Gao, Libo Dong, Jianfang Zhou, Ye Zhang, Jie Dong, Hong Bo, Kun Qin and Yuelong Shu
Author Affiliations
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Virology Journal 2013, 10:289 doi:10.1186/1743-422X-10-289
Published: 15 September 2013
Abstract (provisional)
Background
In the spring of 2013, a novel avian-origin influenza A (H7N9) virus in Eastern China emerged causing human infections. Concerns that a new influenza pandemic could occur were raised. The potential effect of chemical agents and physical conditions on inactivation of the novel avian influenza H7N9 virus had not been assessed.
Methods
To determine the inactivation effectiveness of the novel avian influenza A (H7N9) virus under various physical conditions and chemical treatments, two H7N9 viruses A/Anhui/1/2013 and A/Shanghai/1/2013 were treated by varied temperatures, ultraviolet light, varied pHs and different disinfectants. The viruses with107.7 EID50 were exposed to physical conditions (temperature, ultraviolet light and pH) or treated with commercial chemical agents (Sodium Hypochlorite, Virkon(R)-S, and Ethanol) respectively. After these treatments, the viruses were inoculated in SPF embryonated chicken eggs, the allantoic fluid was collected after 72--96 hours culture at 35[degree sign]C and tested by haemagglutination assay.
Results
Both of the tested viruses could tolerate conditions under 56[degree sign]C for 15 minutes or 60[degree sign]C for 5 minutes, but their infectivity was completely lost under 56[degree sign]C for 30 minutes, 65[degree sign]C for 10 minutes, 70[degree sign]C,75[degree sign]C and 100[degree sign]C for 1 minute. It was also observed that the H7N9 viruses lost their infectivity totally after exposure of ultraviolet light irradiation for 30 minutes or longer time. Additionally, the viruses were completely inactivated at pH less than 2 for 0.5 hour or pH 3 for 24 hours, however, viruses remained infectious under pH treatment of 4--12 for 24 hours. The viruses were totally disinfected when treated with Sodium Hypochlorite, Virkon(R)-S and Ethanol at recommended concentrations after only 5minutes.
Conclusions
The novel avian influenza A (H7N9) virus can be inactivated under some physical conditions or with chemical treatments, but they present high tolerance to moderately acidic or higher alkali conditions. The results provided the essential information for public health intervention of novel H7N9 avian influenza outbreak.
full article
http://www.virologyj.com/content/pdf/1743-422X-10-289.pdf
Shumei Zou, Junfeng Guo, Rongbao Gao, Libo Dong, Jianfang Zhou, Ye Zhang, Jie Dong, Hong Bo, Kun Qin and Yuelong Shu
Author Affiliations
For all author emails, please log on.
Virology Journal 2013, 10:289 doi:10.1186/1743-422X-10-289
Published: 15 September 2013
Abstract (provisional)
Background
In the spring of 2013, a novel avian-origin influenza A (H7N9) virus in Eastern China emerged causing human infections. Concerns that a new influenza pandemic could occur were raised. The potential effect of chemical agents and physical conditions on inactivation of the novel avian influenza H7N9 virus had not been assessed.
Methods
To determine the inactivation effectiveness of the novel avian influenza A (H7N9) virus under various physical conditions and chemical treatments, two H7N9 viruses A/Anhui/1/2013 and A/Shanghai/1/2013 were treated by varied temperatures, ultraviolet light, varied pHs and different disinfectants. The viruses with107.7 EID50 were exposed to physical conditions (temperature, ultraviolet light and pH) or treated with commercial chemical agents (Sodium Hypochlorite, Virkon(R)-S, and Ethanol) respectively. After these treatments, the viruses were inoculated in SPF embryonated chicken eggs, the allantoic fluid was collected after 72--96 hours culture at 35[degree sign]C and tested by haemagglutination assay.
Results
Both of the tested viruses could tolerate conditions under 56[degree sign]C for 15 minutes or 60[degree sign]C for 5 minutes, but their infectivity was completely lost under 56[degree sign]C for 30 minutes, 65[degree sign]C for 10 minutes, 70[degree sign]C,75[degree sign]C and 100[degree sign]C for 1 minute. It was also observed that the H7N9 viruses lost their infectivity totally after exposure of ultraviolet light irradiation for 30 minutes or longer time. Additionally, the viruses were completely inactivated at pH less than 2 for 0.5 hour or pH 3 for 24 hours, however, viruses remained infectious under pH treatment of 4--12 for 24 hours. The viruses were totally disinfected when treated with Sodium Hypochlorite, Virkon(R)-S and Ethanol at recommended concentrations after only 5minutes.
Conclusions
The novel avian influenza A (H7N9) virus can be inactivated under some physical conditions or with chemical treatments, but they present high tolerance to moderately acidic or higher alkali conditions. The results provided the essential information for public health intervention of novel H7N9 avian influenza outbreak.
full article
http://www.virologyj.com/content/pdf/1743-422X-10-289.pdf