tetano
Editor, Senior Moderator
Viruses
. 2021 Aug 18;13(8):1634.
doi: 10.3390/v13081634.
Serological Screening for Antibodies against SARS-CoV-2 in Dutch Shelter Cats
W J R van der Leij[SUP] 1 [/SUP], Els M Broens[SUP] 2 [/SUP], Jan Willem Hesselink[SUP] 3 [/SUP], Nancy Schuurman[SUP] 4 [/SUP], Johannes C M Vernooij[SUP] 5 [/SUP], Herman F Egberink[SUP] 4 [/SUP]
Affiliations
Abstract
The COVID-19 pandemic raised concerns that companion animals might be infected with, and could become a reservoir of, SARS-CoV-2. As cats are popular pets and susceptible to Coronavirus, we investigated the seroprevalence of SARS-CoV-2 antibodies in shelter cats housed in Dutch animal shelters during the COVID-19 pandemic. In this large-scale cross-sectional study, serum samples of shelter cats were collected during the second wave of human COVID-19 infections in The Netherlands. Seroprevalence was determined by using an indirect protein-based ELISA validated for cats, and a Virus Neutralization Test (VNT) as confirmation. To screen for feline SARS-CoV-2 shedding, oropharyngeal and rectal swabs of cats positive for ELISA and/or VNT were analyzed using PCR tests. In 28 Dutch animal shelters, 240 shelter cats were convenience sampled. Two of these cats (0.8%; CI 95%: 0.1-3.0%) were seropositive, as evidenced by the presence of SARS-CoV-2 neutralizing antibodies. The seropositive animals tested PCR negative for SARS-CoV-2. Based on the results of this study, it is unlikely that shelter cats could be a reservoir of SARS-CoV-2 or pose a (significant) risk to public health.
Keywords: Coronavirus disease 2019 (COVID-19); animal shelter; neutralizing SARS-CoV-2 antibody; one health; serology; seroprevalence; shelter medicine; titer.
. 2021 Aug 18;13(8):1634.
doi: 10.3390/v13081634.
Serological Screening for Antibodies against SARS-CoV-2 in Dutch Shelter Cats
W J R van der Leij[SUP] 1 [/SUP], Els M Broens[SUP] 2 [/SUP], Jan Willem Hesselink[SUP] 3 [/SUP], Nancy Schuurman[SUP] 4 [/SUP], Johannes C M Vernooij[SUP] 5 [/SUP], Herman F Egberink[SUP] 4 [/SUP]
Affiliations
- PMID: 34452497
- DOI: 10.3390/v13081634
Abstract
The COVID-19 pandemic raised concerns that companion animals might be infected with, and could become a reservoir of, SARS-CoV-2. As cats are popular pets and susceptible to Coronavirus, we investigated the seroprevalence of SARS-CoV-2 antibodies in shelter cats housed in Dutch animal shelters during the COVID-19 pandemic. In this large-scale cross-sectional study, serum samples of shelter cats were collected during the second wave of human COVID-19 infections in The Netherlands. Seroprevalence was determined by using an indirect protein-based ELISA validated for cats, and a Virus Neutralization Test (VNT) as confirmation. To screen for feline SARS-CoV-2 shedding, oropharyngeal and rectal swabs of cats positive for ELISA and/or VNT were analyzed using PCR tests. In 28 Dutch animal shelters, 240 shelter cats were convenience sampled. Two of these cats (0.8%; CI 95%: 0.1-3.0%) were seropositive, as evidenced by the presence of SARS-CoV-2 neutralizing antibodies. The seropositive animals tested PCR negative for SARS-CoV-2. Based on the results of this study, it is unlikely that shelter cats could be a reservoir of SARS-CoV-2 or pose a (significant) risk to public health.
Keywords: Coronavirus disease 2019 (COVID-19); animal shelter; neutralizing SARS-CoV-2 antibody; one health; serology; seroprevalence; shelter medicine; titer.