tetano
Editor, Senior Moderator
Viruses
. 2022 Feb 4;14(2):322.
doi: 10.3390/v14020322.
Secondary Structure of Subgenomic RNA M of SARS-CoV-2
Marta Soszynska-Jozwiak[SUP] 1 [/SUP], Agnieszka Ruszkowska[SUP] 1 [/SUP], Ryszard Kierzek[SUP] 1 [/SUP], Collin A O'Leary[SUP] 2 [/SUP], Walter N Moss[SUP] 2 [/SUP], Elzbieta Kierzek[SUP] 1 [/SUP]
Affiliations
Abstract
SARS-CoV-2 belongs to the Coronavirinae family. Like other coronaviruses, SARS-CoV-2 is enveloped and possesses a positive-sense, single-stranded RNA genome of ~30 kb. Genomic RNA is used as the template for replication and transcription. During these processes, positive-sense genomic RNA (gRNA) and subgenomic RNAs (sgRNAs) are created. Several studies presented the importance of the genomic RNA secondary structure in SARS-CoV-2 replication. However, the structure of sgRNAs has remained largely unsolved so far. In this study, we probed the sgRNA M model of SARS-CoV-2 in vitro. The presented model molecule includes 5'UTR and a coding sequence of gene M. This is the first experimentally informed secondary structure model of sgRNA M, which presents features likely to be important in sgRNA M function. The knowledge of sgRNA M structure provides insights to better understand virus biology and could be used for designing new therapeutics.
Keywords: COVID-19; RNA structure; SARS-CoV-2; chemical mapping; sgRNA; sgRNA M; subgenomic RNAs.
. 2022 Feb 4;14(2):322.
doi: 10.3390/v14020322.
Secondary Structure of Subgenomic RNA M of SARS-CoV-2
Marta Soszynska-Jozwiak[SUP] 1 [/SUP], Agnieszka Ruszkowska[SUP] 1 [/SUP], Ryszard Kierzek[SUP] 1 [/SUP], Collin A O'Leary[SUP] 2 [/SUP], Walter N Moss[SUP] 2 [/SUP], Elzbieta Kierzek[SUP] 1 [/SUP]
Affiliations
- PMID: 35215915
- DOI: 10.3390/v14020322
Abstract
SARS-CoV-2 belongs to the Coronavirinae family. Like other coronaviruses, SARS-CoV-2 is enveloped and possesses a positive-sense, single-stranded RNA genome of ~30 kb. Genomic RNA is used as the template for replication and transcription. During these processes, positive-sense genomic RNA (gRNA) and subgenomic RNAs (sgRNAs) are created. Several studies presented the importance of the genomic RNA secondary structure in SARS-CoV-2 replication. However, the structure of sgRNAs has remained largely unsolved so far. In this study, we probed the sgRNA M model of SARS-CoV-2 in vitro. The presented model molecule includes 5'UTR and a coding sequence of gene M. This is the first experimentally informed secondary structure model of sgRNA M, which presents features likely to be important in sgRNA M function. The knowledge of sgRNA M structure provides insights to better understand virus biology and could be used for designing new therapeutics.
Keywords: COVID-19; RNA structure; SARS-CoV-2; chemical mapping; sgRNA; sgRNA M; subgenomic RNAs.