tetano
Editor, Senior Moderator
Viruses
. 2021 Jun 12;13(6):1135.
doi: 10.3390/v13061135.
Previous SARS-CoV-2 Infection Increases B.1.1.7 Cross-Neutralization by Vaccinated Individuals
Benjamin Trinité[SUP] 1 [/SUP], Edwards Pradenas[SUP] 1 [/SUP], Silvia Marfil[SUP] 1 [/SUP], Carla Rovirosa[SUP] 1 [/SUP], Víctor Urrea[SUP] 1 [/SUP], Ferran Tarrés-Freixas[SUP] 1 [/SUP], Raquel Ortiz[SUP] 1 [/SUP], Jordi Rodon[SUP] 2 [/SUP], Júlia Vergara-Alert[SUP] 2 [/SUP], Joaquim Segalés[SUP] 3 4 [/SUP], Victor Guallar[SUP] 5 6 [/SUP], Rosalba Lepore[SUP] 5 [/SUP], Nuria Izquierdo-Useros[SUP] 1 [/SUP], Glòria Trujillo[SUP] 7 [/SUP], Jaume Trapé[SUP] 7 [/SUP], Carolina González-Fernández[SUP] 7 [/SUP], Antonia Flor[SUP] 7 [/SUP], Rafel Pérez-Vidal[SUP] 7 [/SUP], Ruth Toledo[SUP] 8 [/SUP], Anna Chamorro[SUP] 8 [/SUP], Roger Paredes[SUP] 1 8 [/SUP], Ignacio Blanco[SUP] 9 [/SUP], Eulàlia Grau[SUP] 1 [/SUP], Marta Massanella[SUP] 1 [/SUP], Jorge Carrillo[SUP] 1 [/SUP], Bonaventura Clotet[SUP] 1 8 10 [/SUP], Julià Blanco[SUP] 1 10 [/SUP]
Affiliations
Abstract
With the spread of new variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), there is a need to assess the protection conferred by both previous infections and current vaccination. Here we tested the neutralizing activity of infected and/or vaccinated individuals against pseudoviruses expressing the spike of the original SARS-CoV-2 isolate Wuhan-Hu-1 (WH1), the D614G mutant and the B.1.1.7 variant. Our data show that parameters of natural infection (time from infection and nature of the infecting variant) determined cross-neutralization. Uninfected vaccinees showed a small reduction in neutralization against the B.1.1.7 variant compared to both the WH1 strain and the D614G mutant. Interestingly, upon vaccination, previously infected individuals developed more robust neutralizing responses against B.1.1.7, suggesting that vaccines can boost the neutralization breadth conferred by natural infection.
Keywords: B.1.1.7 variant; SARS-CoV-2; humoral response; neutralization; pseudovirus.
. 2021 Jun 12;13(6):1135.
doi: 10.3390/v13061135.
Previous SARS-CoV-2 Infection Increases B.1.1.7 Cross-Neutralization by Vaccinated Individuals
Benjamin Trinité[SUP] 1 [/SUP], Edwards Pradenas[SUP] 1 [/SUP], Silvia Marfil[SUP] 1 [/SUP], Carla Rovirosa[SUP] 1 [/SUP], Víctor Urrea[SUP] 1 [/SUP], Ferran Tarrés-Freixas[SUP] 1 [/SUP], Raquel Ortiz[SUP] 1 [/SUP], Jordi Rodon[SUP] 2 [/SUP], Júlia Vergara-Alert[SUP] 2 [/SUP], Joaquim Segalés[SUP] 3 4 [/SUP], Victor Guallar[SUP] 5 6 [/SUP], Rosalba Lepore[SUP] 5 [/SUP], Nuria Izquierdo-Useros[SUP] 1 [/SUP], Glòria Trujillo[SUP] 7 [/SUP], Jaume Trapé[SUP] 7 [/SUP], Carolina González-Fernández[SUP] 7 [/SUP], Antonia Flor[SUP] 7 [/SUP], Rafel Pérez-Vidal[SUP] 7 [/SUP], Ruth Toledo[SUP] 8 [/SUP], Anna Chamorro[SUP] 8 [/SUP], Roger Paredes[SUP] 1 8 [/SUP], Ignacio Blanco[SUP] 9 [/SUP], Eulàlia Grau[SUP] 1 [/SUP], Marta Massanella[SUP] 1 [/SUP], Jorge Carrillo[SUP] 1 [/SUP], Bonaventura Clotet[SUP] 1 8 10 [/SUP], Julià Blanco[SUP] 1 10 [/SUP]
Affiliations
- PMID: 34204754
- DOI: 10.3390/v13061135
Abstract
With the spread of new variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), there is a need to assess the protection conferred by both previous infections and current vaccination. Here we tested the neutralizing activity of infected and/or vaccinated individuals against pseudoviruses expressing the spike of the original SARS-CoV-2 isolate Wuhan-Hu-1 (WH1), the D614G mutant and the B.1.1.7 variant. Our data show that parameters of natural infection (time from infection and nature of the infecting variant) determined cross-neutralization. Uninfected vaccinees showed a small reduction in neutralization against the B.1.1.7 variant compared to both the WH1 strain and the D614G mutant. Interestingly, upon vaccination, previously infected individuals developed more robust neutralizing responses against B.1.1.7, suggesting that vaccines can boost the neutralization breadth conferred by natural infection.
Keywords: B.1.1.7 variant; SARS-CoV-2; humoral response; neutralization; pseudovirus.