tetano
Editor, Senior Moderator
Viruses
. 2023 Mar 20;15(3):790.
doi: 10.3390/v15030790.
One Health Surveillance Highlights Circulation of Viruses with Zoonotic Potential in Bats, Pigs, and Humans in Viet Nam
Alice Latinne[SUP] 1 2 [/SUP], Nguyen Thi Thanh Nga[SUP] 1 [/SUP], Nguyen Van Long[SUP] 1 [/SUP], Pham Thi Bich Ngoc[SUP] 1 [/SUP], Hoang Bich Thuy[SUP] 1 [/SUP], Predict Consortium, Nguyen Van Long[SUP] 3 [/SUP], Pham Thanh Long[SUP] 3 [/SUP], Nguyen Thanh Phuong[SUP] 4 [/SUP], Le Tin Vinh Quang[SUP] 4 [/SUP], Nguyen Tung[SUP] 3 [/SUP], Vu Sinh Nam[SUP] 5 [/SUP], Vu Trong Duoc[SUP] 5 [/SUP], Nguyen Duc Thinh[SUP] 5 [/SUP], Randal Schoepp[SUP] 6 [/SUP], Keersten Ricks[SUP] 6 [/SUP], Ken Inui[SUP] 7 [/SUP], Pawin Padungtod[SUP] 7 [/SUP], Christine K Johnson[SUP] 8 [/SUP], Jonna A K Mazet[SUP] 8 [/SUP], Chris Walzer[SUP] 2 9 [/SUP], Sarah H Olson[SUP] 2 [/SUP], Amanda E Fine[SUP] 1 2 [/SUP]
Affiliations
Abstract
A One Health cross-sectoral surveillance approach was implemented to screen biological samples from bats, pigs, and humans at high-risk interfaces for zoonotic viral spillover for five viral families with zoonotic potential in Viet Nam. Over 1600 animal and human samples from bat guano harvesting sites, natural bat roosts, and pig farming operations were tested for coronaviruses (CoVs), paramyxoviruses, influenza viruses, filoviruses and flaviviruses using consensus PCR assays. Human samples were also tested using immunoassays to detect antibodies against eight virus groups. Significant viral diversity, including CoVs closely related to ancestors of pig pathogens, was detected in bats roosting at the human-animal interfaces, illustrating the high risk for CoV spillover from bats to pigs in Viet Nam, where pig density is very high. Season and reproductive period were significantly associated with the detection of bat CoVs, with site-specific effects. Phylogeographic analysis indicated localized viral transmission among pig farms. Our limited human sampling did not detect any known zoonotic bat viruses in human communities living close to the bat cave and harvesting bat guano, but our serological assays showed possible previous exposure to Marburg virus-like (Filoviridae), Crimean-Congo hemorrhagic fever virus-like (Bunyaviridae) viruses and flaviviruses. Targeted and coordinated One Health surveillance helped uncover this viral pathogen emergence hotspot.
Keywords: One Health; bats; coronavirus; influenza; livestock; paramyxovirus; pigs; spillover; surveillance; zoonoses.
. 2023 Mar 20;15(3):790.
doi: 10.3390/v15030790.
One Health Surveillance Highlights Circulation of Viruses with Zoonotic Potential in Bats, Pigs, and Humans in Viet Nam
Alice Latinne[SUP] 1 2 [/SUP], Nguyen Thi Thanh Nga[SUP] 1 [/SUP], Nguyen Van Long[SUP] 1 [/SUP], Pham Thi Bich Ngoc[SUP] 1 [/SUP], Hoang Bich Thuy[SUP] 1 [/SUP], Predict Consortium, Nguyen Van Long[SUP] 3 [/SUP], Pham Thanh Long[SUP] 3 [/SUP], Nguyen Thanh Phuong[SUP] 4 [/SUP], Le Tin Vinh Quang[SUP] 4 [/SUP], Nguyen Tung[SUP] 3 [/SUP], Vu Sinh Nam[SUP] 5 [/SUP], Vu Trong Duoc[SUP] 5 [/SUP], Nguyen Duc Thinh[SUP] 5 [/SUP], Randal Schoepp[SUP] 6 [/SUP], Keersten Ricks[SUP] 6 [/SUP], Ken Inui[SUP] 7 [/SUP], Pawin Padungtod[SUP] 7 [/SUP], Christine K Johnson[SUP] 8 [/SUP], Jonna A K Mazet[SUP] 8 [/SUP], Chris Walzer[SUP] 2 9 [/SUP], Sarah H Olson[SUP] 2 [/SUP], Amanda E Fine[SUP] 1 2 [/SUP]
Affiliations
- PMID: 36992498
- DOI: 10.3390/v15030790
Abstract
A One Health cross-sectoral surveillance approach was implemented to screen biological samples from bats, pigs, and humans at high-risk interfaces for zoonotic viral spillover for five viral families with zoonotic potential in Viet Nam. Over 1600 animal and human samples from bat guano harvesting sites, natural bat roosts, and pig farming operations were tested for coronaviruses (CoVs), paramyxoviruses, influenza viruses, filoviruses and flaviviruses using consensus PCR assays. Human samples were also tested using immunoassays to detect antibodies against eight virus groups. Significant viral diversity, including CoVs closely related to ancestors of pig pathogens, was detected in bats roosting at the human-animal interfaces, illustrating the high risk for CoV spillover from bats to pigs in Viet Nam, where pig density is very high. Season and reproductive period were significantly associated with the detection of bat CoVs, with site-specific effects. Phylogeographic analysis indicated localized viral transmission among pig farms. Our limited human sampling did not detect any known zoonotic bat viruses in human communities living close to the bat cave and harvesting bat guano, but our serological assays showed possible previous exposure to Marburg virus-like (Filoviridae), Crimean-Congo hemorrhagic fever virus-like (Bunyaviridae) viruses and flaviviruses. Targeted and coordinated One Health surveillance helped uncover this viral pathogen emergence hotspot.
Keywords: One Health; bats; coronavirus; influenza; livestock; paramyxovirus; pigs; spillover; surveillance; zoonoses.