tetano
Editor, Senior Moderator
Viruses
. 2022 Feb 28;14(3):501.
doi: 10.3390/v14030501.
Effect of Different Adjuvants on Immune Responses Elicited by Protein-Based Subunit Vaccines against SARS-CoV-2 and Its Delta Variant
Naru Zhang[SUP] 1 [/SUP], Qianting Ji[SUP] 1 [/SUP], Zezhong Liu[SUP] 2 [/SUP], Kaiming Tang[SUP] 3 [/SUP], Yubin Xie[SUP] 3 [/SUP], Kangchen Li[SUP] 1 [/SUP], Jie Zhou[SUP] 2 [/SUP], Sisi Li[SUP] 1 [/SUP], Haotian Shang[SUP] 1 [/SUP], Zecan Shi[SUP] 1 [/SUP], Tianyu Zheng[SUP] 1 [/SUP], Jiawei Yao[SUP] 1 [/SUP], Lu Lu[SUP] 2 [/SUP], Shuofeng Yuan[SUP] 3 [/SUP], Shibo Jiang[SUP] 2 [/SUP]
Affiliations
Abstract
The global pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become more serious because of the continuous emergence of variants of concern (VOC), thus calling for the development of broad-spectrum vaccines with greater efficacy. Adjuvants play important roles in enhancing the immunogenicity of protein-based subunit vaccines. In this study, we compared the effect of three adjuvants, including aluminum, nanoparticle manganese and MF59, on the immunogenicity of three protein-based COVID-19 vaccine candidates, including RBD-Fc, RBD and S-trimer. We found that the nanoparticle manganese adjuvant elicited the highest titers of SARS-CoV-2 RBD-specific IgG, IgG1 and IgG2a, as well as neutralizing antibodies against infection by pseudotyped SARS-CoV-2 and its Delta variant. What is more, the nanoparticle manganese adjuvant effectively reduced the viral load of the authentic SARS-CoV-2 and Delta variant in the cell culture supernatants. These results suggest that nanoparticle manganese, known to facilitate cGAS-STING activation, is an optimal adjuvant for protein-based COVID-19 subunit vaccines.
Keywords: COVID-19; MF59; SARS-CoV-2; aluminum; nanoparticle manganese; subunit vaccines; variants.
. 2022 Feb 28;14(3):501.
doi: 10.3390/v14030501.
Effect of Different Adjuvants on Immune Responses Elicited by Protein-Based Subunit Vaccines against SARS-CoV-2 and Its Delta Variant
Naru Zhang[SUP] 1 [/SUP], Qianting Ji[SUP] 1 [/SUP], Zezhong Liu[SUP] 2 [/SUP], Kaiming Tang[SUP] 3 [/SUP], Yubin Xie[SUP] 3 [/SUP], Kangchen Li[SUP] 1 [/SUP], Jie Zhou[SUP] 2 [/SUP], Sisi Li[SUP] 1 [/SUP], Haotian Shang[SUP] 1 [/SUP], Zecan Shi[SUP] 1 [/SUP], Tianyu Zheng[SUP] 1 [/SUP], Jiawei Yao[SUP] 1 [/SUP], Lu Lu[SUP] 2 [/SUP], Shuofeng Yuan[SUP] 3 [/SUP], Shibo Jiang[SUP] 2 [/SUP]
Affiliations
- PMID: 35336907
- DOI: 10.3390/v14030501
Abstract
The global pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become more serious because of the continuous emergence of variants of concern (VOC), thus calling for the development of broad-spectrum vaccines with greater efficacy. Adjuvants play important roles in enhancing the immunogenicity of protein-based subunit vaccines. In this study, we compared the effect of three adjuvants, including aluminum, nanoparticle manganese and MF59, on the immunogenicity of three protein-based COVID-19 vaccine candidates, including RBD-Fc, RBD and S-trimer. We found that the nanoparticle manganese adjuvant elicited the highest titers of SARS-CoV-2 RBD-specific IgG, IgG1 and IgG2a, as well as neutralizing antibodies against infection by pseudotyped SARS-CoV-2 and its Delta variant. What is more, the nanoparticle manganese adjuvant effectively reduced the viral load of the authentic SARS-CoV-2 and Delta variant in the cell culture supernatants. These results suggest that nanoparticle manganese, known to facilitate cGAS-STING activation, is an optimal adjuvant for protein-based COVID-19 subunit vaccines.
Keywords: COVID-19; MF59; SARS-CoV-2; aluminum; nanoparticle manganese; subunit vaccines; variants.