tetano
Editor, Senior Moderator
Viruses
. 2022 Dec 22;15(1):38.
doi: 10.3390/v15010038.
Clade 2.3.4.4b H5N8 Subtype Avian Influenza Viruses Were Identified from the Common Crane Wintering in Yunnan Province, China
Qinhong Yang[SUP] 1 [/SUP], Xiaoyan Xue[SUP] 1 [/SUP], Zhenxing Zhang[SUP] 2 [/SUP], Ming J Wu[SUP] 3 [/SUP], Jia Ji[SUP] 1 [/SUP], Wei Wang[SUP] 1 [/SUP], Hongbin Yin[SUP] 4 [/SUP], Suhua Li[SUP] 1 [/SUP], Hongyang Dai[SUP] 5 [/SUP], Bofang Duan[SUP] 6 [/SUP], Qiang Liu[SUP] 1 [/SUP], Jianling Song[SUP] 2 [/SUP]
Affiliations
Abstract
The seasonal migration of wild aquatic birds plays a critical role in the maintenance, transmission, and incursion of the avian influenza virus (AIV). AIV surveillance was performed during 2020-2021 in two national nature reserves with abundant wild bird resources in Yunnan, China. Four H5N8 AIVs isolates from the common crane were identified by next-generation sequencing. Phylogenetic analysis demonstrated that all eight gene segments of these H5N8 AIVs belonged to clade 2.3.4.4b high-pathogenic AIV (HPAIV) and shared high nucleotide sequence similarity with the strains isolated in Hubei, China, and Siberia, Russia, in 2020-2021. The H5N8 HPAIVs from common cranes were characterized by both human and avian dual-receptor specificity in the hemagglutinin (HA) protein. Moreover, possessing the substitutions contributes to overcoming transmission barriers of mammalian hosts in polymerase basic 2 (PB2), polymerase basic protein 1 (PB1), and polymerase acid (PA), and exhibiting the long stalk in the neck region of the neuraminidase (NA) protein contributes to adaptation in wild birds. Monitoring AIVs in migratory birds, at stopover sites and in their primary habitats, i.e., breeding or wintering grounds, could provide insight into potential zoonosis caused by AIVs.
Keywords: H5N8; avian influenza virus; genetic analysis; wild bird.
. 2022 Dec 22;15(1):38.
doi: 10.3390/v15010038.
Clade 2.3.4.4b H5N8 Subtype Avian Influenza Viruses Were Identified from the Common Crane Wintering in Yunnan Province, China
Qinhong Yang[SUP] 1 [/SUP], Xiaoyan Xue[SUP] 1 [/SUP], Zhenxing Zhang[SUP] 2 [/SUP], Ming J Wu[SUP] 3 [/SUP], Jia Ji[SUP] 1 [/SUP], Wei Wang[SUP] 1 [/SUP], Hongbin Yin[SUP] 4 [/SUP], Suhua Li[SUP] 1 [/SUP], Hongyang Dai[SUP] 5 [/SUP], Bofang Duan[SUP] 6 [/SUP], Qiang Liu[SUP] 1 [/SUP], Jianling Song[SUP] 2 [/SUP]
Affiliations
- PMID: 36680078
- PMCID: PMC9863098
- DOI: 10.3390/v15010038
Abstract
The seasonal migration of wild aquatic birds plays a critical role in the maintenance, transmission, and incursion of the avian influenza virus (AIV). AIV surveillance was performed during 2020-2021 in two national nature reserves with abundant wild bird resources in Yunnan, China. Four H5N8 AIVs isolates from the common crane were identified by next-generation sequencing. Phylogenetic analysis demonstrated that all eight gene segments of these H5N8 AIVs belonged to clade 2.3.4.4b high-pathogenic AIV (HPAIV) and shared high nucleotide sequence similarity with the strains isolated in Hubei, China, and Siberia, Russia, in 2020-2021. The H5N8 HPAIVs from common cranes were characterized by both human and avian dual-receptor specificity in the hemagglutinin (HA) protein. Moreover, possessing the substitutions contributes to overcoming transmission barriers of mammalian hosts in polymerase basic 2 (PB2), polymerase basic protein 1 (PB1), and polymerase acid (PA), and exhibiting the long stalk in the neck region of the neuraminidase (NA) protein contributes to adaptation in wild birds. Monitoring AIVs in migratory birds, at stopover sites and in their primary habitats, i.e., breeding or wintering grounds, could provide insight into potential zoonosis caused by AIVs.
Keywords: H5N8; avian influenza virus; genetic analysis; wild bird.