tetano
Editor, Senior Moderator
Viruses
. 2021 Jun 5;13(6):1080.
doi: 10.3390/v13061080.
Broad-Based Influenza-Specific CD8 [SUP]+[/SUP] T Cell Response without the Typical Immunodominance Hierarchy and Its Potential Implication
Miaojuan Huang[SUP] 1 [/SUP], Rong Xu[SUP] 2 [/SUP], Cristina Triffon[SUP] 1 [/SUP], Nicole Mifsud[SUP] 3 [/SUP], Weisan Chen[SUP] 1 [/SUP]
Affiliations
Abstract
Syngeneic murine systems have pre-fixed MHC, making them an imperfect model for investigating the impact of MHC polymorphism on immunodominance in influenza A virus (IAV) infections. To date, there are few studies focusing on MHC allelic differences and its impact on immunodominance even though it is well documented that an individual's HLA plays a significant role in determining immunodominance hierarchy. Here, we describe a broad-based CD8[SUP]+[/SUP] T cell response in a healthy individual to IAV infection rather than a typical immunodominance hierarchy. We used a systematic antigen screen approach combined with epitope prediction to study such a broad CD8[SUP]+[/SUP] T cell response to IAV infection. We show CD8[SUP]+[/SUP] T cell responses to nine IAV proteins and identify their minimal epitope sequences. These epitopes are restricted to HLA-B*44:03, HLA-A*24:02 and HLA-A*33:03 and seven out of the nine epitopes are novel (NP[SUB]319-330[/SUB][SUP]#[/SUP] (known and demonstrated minimal epitope positions are subscripted; otherwise, amino acid positions are shown as normal text (for example NP 319-330 or NP 313-330)), M1[SUB]124-134[/SUB], M2[SUB]7-15[/SUB], NA[SUB]337-346[/SUB], PB2[SUB]39-49[/SUB], HA[SUB]445-453[/SUB] and NS1[SUB]195-203[/SUB]). Additionally, most of these novel epitopes are highly conserved among H1N1 and H3N2 strains that circulated in Australia and other parts of the world.
Keywords: CD8+ T cell epitope; HLA; antigen presentation; immunodominance; influenza A virus.
. 2021 Jun 5;13(6):1080.
doi: 10.3390/v13061080.
Broad-Based Influenza-Specific CD8 [SUP]+[/SUP] T Cell Response without the Typical Immunodominance Hierarchy and Its Potential Implication
Miaojuan Huang[SUP] 1 [/SUP], Rong Xu[SUP] 2 [/SUP], Cristina Triffon[SUP] 1 [/SUP], Nicole Mifsud[SUP] 3 [/SUP], Weisan Chen[SUP] 1 [/SUP]
Affiliations
- PMID: 34198851
- DOI: 10.3390/v13061080
Abstract
Syngeneic murine systems have pre-fixed MHC, making them an imperfect model for investigating the impact of MHC polymorphism on immunodominance in influenza A virus (IAV) infections. To date, there are few studies focusing on MHC allelic differences and its impact on immunodominance even though it is well documented that an individual's HLA plays a significant role in determining immunodominance hierarchy. Here, we describe a broad-based CD8[SUP]+[/SUP] T cell response in a healthy individual to IAV infection rather than a typical immunodominance hierarchy. We used a systematic antigen screen approach combined with epitope prediction to study such a broad CD8[SUP]+[/SUP] T cell response to IAV infection. We show CD8[SUP]+[/SUP] T cell responses to nine IAV proteins and identify their minimal epitope sequences. These epitopes are restricted to HLA-B*44:03, HLA-A*24:02 and HLA-A*33:03 and seven out of the nine epitopes are novel (NP[SUB]319-330[/SUB][SUP]#[/SUP] (known and demonstrated minimal epitope positions are subscripted; otherwise, amino acid positions are shown as normal text (for example NP 319-330 or NP 313-330)), M1[SUB]124-134[/SUB], M2[SUB]7-15[/SUB], NA[SUB]337-346[/SUB], PB2[SUB]39-49[/SUB], HA[SUB]445-453[/SUB] and NS1[SUB]195-203[/SUB]). Additionally, most of these novel epitopes are highly conserved among H1N1 and H3N2 strains that circulated in Australia and other parts of the world.
Keywords: CD8+ T cell epitope; HLA; antigen presentation; immunodominance; influenza A virus.