tetano
Editor, Senior Moderator
Viruses. 2018 Jul 5;10(7). pii: E356. doi: 10.3390/v10070356.
[h=1]Anti-Influenza A Viral Butenolide from Streptomyces sp. Smu03 Inhabiting the Intestine of Elephas maximus.[/h] Li F[SUP]1,[/SUP][SUP]2[/SUP], Chen D[SUP]3[/SUP], Lu S[SUP]4[/SUP], Yang G[SUP]5[/SUP], Zhang X[SUP]6[/SUP], Chen Z[SUP]7[/SUP], Fan S[SUP]8[/SUP], Wu S[SUP]9[/SUP], He J[SUP]10[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Actinobacteria are a phylum of bacteria known for their potential in producing structurally diversified natural products that are always associated with a broad range of biological activities. In this paper, using an H5N1 pseudo-typed virus drug screening system combined with a bioassay guided purification approach, an antiviral butanolide (1) was identified from the culture broth of Streptomyces sp. SMU03, a bacterium isolated from the feces of Elephas maximus in Yunnan province, China. This compound displayed broad and potent activity against a panel of influenza viruses including H1N1 and H3N2 subtypes, as well as influenza B virus and clinical isolates with half maximal inhibitory concentration values (IC[SUB]50[/SUB]) in the range of 0.29 to 12 µg/mL. In addition, 1 was also active against oseltamivir-resistant influenza virus strain of A/PR/8/34 with NA-H274Y mutation. Studies on the detailed modes of action suggested that 1 functioned by interfering with the fusogenic process of hemagglutinin (HA) of influenza A virus (IAV), thereby blocking the entry of virus into host cells. Furthermore, the anti-IAV activity of 1 was assessed with infected BALB/c mice, of which the appearance, weight, and histopathological changes in the infected lungs were significantly alleviated compared with the no-drug-treated group. Conclusively, these results provide evidence that natural products derived from microbes residing in animal intestines might be a good source for antiviral drug discovery.
[h=4]KEYWORDS:[/h] Streptomyces sp.; anti-influenza A viruses; butenolide; hemagglutinin
PMID: 29976861 DOI: 10.3390/v10070356
Free full text
[h=1]Anti-Influenza A Viral Butenolide from Streptomyces sp. Smu03 Inhabiting the Intestine of Elephas maximus.[/h] Li F[SUP]1,[/SUP][SUP]2[/SUP], Chen D[SUP]3[/SUP], Lu S[SUP]4[/SUP], Yang G[SUP]5[/SUP], Zhang X[SUP]6[/SUP], Chen Z[SUP]7[/SUP], Fan S[SUP]8[/SUP], Wu S[SUP]9[/SUP], He J[SUP]10[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Actinobacteria are a phylum of bacteria known for their potential in producing structurally diversified natural products that are always associated with a broad range of biological activities. In this paper, using an H5N1 pseudo-typed virus drug screening system combined with a bioassay guided purification approach, an antiviral butanolide (1) was identified from the culture broth of Streptomyces sp. SMU03, a bacterium isolated from the feces of Elephas maximus in Yunnan province, China. This compound displayed broad and potent activity against a panel of influenza viruses including H1N1 and H3N2 subtypes, as well as influenza B virus and clinical isolates with half maximal inhibitory concentration values (IC[SUB]50[/SUB]) in the range of 0.29 to 12 µg/mL. In addition, 1 was also active against oseltamivir-resistant influenza virus strain of A/PR/8/34 with NA-H274Y mutation. Studies on the detailed modes of action suggested that 1 functioned by interfering with the fusogenic process of hemagglutinin (HA) of influenza A virus (IAV), thereby blocking the entry of virus into host cells. Furthermore, the anti-IAV activity of 1 was assessed with infected BALB/c mice, of which the appearance, weight, and histopathological changes in the infected lungs were significantly alleviated compared with the no-drug-treated group. Conclusively, these results provide evidence that natural products derived from microbes residing in animal intestines might be a good source for antiviral drug discovery.
[h=4]KEYWORDS:[/h] Streptomyces sp.; anti-influenza A viruses; butenolide; hemagglutinin
PMID: 29976861 DOI: 10.3390/v10070356
Free full text