tetano
Editor, Senior Moderator
Virusdisease
. 2024 Sep;35(3):520-524.
doi: 10.1007/s13337-024-00880-z. Epub 2024 Jul 2. Sewage surveillance of SARS-CoV-2 in Tokyo (May 2020-June 2022): method using high-throughput, fully-automated platform
Ryota Kumagai[SUP] 1 [/SUP], Mamiyo Kawakami[SUP] 1 [/SUP], Fumi Kasuya[SUP] 1 [/SUP], Kinji Yamada[SUP] 2 [/SUP], Akihiro Takahashi[SUP] 2 [/SUP], Kouki Murayama[SUP] 2 [/SUP], Kenshi Morita[SUP] 2 [/SUP], Tomoya Kaneyasu[SUP] 3 [/SUP], Naoko Tamai[SUP] 3 [/SUP], Michiya Hasegawa[SUP] 1 [/SUP], Sachiko Harada[SUP] 1 [/SUP], Arisa Amano[SUP] 1 [/SUP], Takushi Fujiwara[SUP] 1 [/SUP], Hirofumi Miyake[SUP] 1 [/SUP], Mami Nagashima[SUP] 1 [/SUP], Kenji Sadamasu[SUP] 1 [/SUP]
Affiliations
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is shed in saliva, sputum, and feces of the novel coronavirus disease 2019 (COVID-19) patients, and SARS-CoV-2 RNA has been detected in sewage. Thus, many regions worldwide have implemented the measurement of SARS-CoV-2 RNA in sewage as a monitoring tool for COVID-19 prevalence. Here, we report the monitoring of two wastewater treatment plants in Tokyo from May 2020 to June 2022 using a fully automated genetic testing method for the solid fraction of sewage. We found that SARS-CoV-2 sewage monitoring using the Panther System with the Aptima SARS-CoV-2 Assay Kit is an effective method of qualitative monitoring. Furthermore, spike gene mutations (N501Y, L452R, E484A) by real-time PCR using test residues were detected in some samples, which reflected the epidemic status. Nevertheless, our findings demonstrate that the use of a fully automated testing platform is effective for SARS-CoV-2 RNA detection in the solid fraction of sewage.
Keywords: COVID-19; SARS-CoV-2; Sewage surveillance; Tokyo.
. 2024 Sep;35(3):520-524.
doi: 10.1007/s13337-024-00880-z. Epub 2024 Jul 2. Sewage surveillance of SARS-CoV-2 in Tokyo (May 2020-June 2022): method using high-throughput, fully-automated platform
Ryota Kumagai[SUP] 1 [/SUP], Mamiyo Kawakami[SUP] 1 [/SUP], Fumi Kasuya[SUP] 1 [/SUP], Kinji Yamada[SUP] 2 [/SUP], Akihiro Takahashi[SUP] 2 [/SUP], Kouki Murayama[SUP] 2 [/SUP], Kenshi Morita[SUP] 2 [/SUP], Tomoya Kaneyasu[SUP] 3 [/SUP], Naoko Tamai[SUP] 3 [/SUP], Michiya Hasegawa[SUP] 1 [/SUP], Sachiko Harada[SUP] 1 [/SUP], Arisa Amano[SUP] 1 [/SUP], Takushi Fujiwara[SUP] 1 [/SUP], Hirofumi Miyake[SUP] 1 [/SUP], Mami Nagashima[SUP] 1 [/SUP], Kenji Sadamasu[SUP] 1 [/SUP]
Affiliations
- PMID: 39464731
- PMCID: PMC11502634 (available on 2025-09-01)
- DOI: 10.1007/s13337-024-00880-z
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is shed in saliva, sputum, and feces of the novel coronavirus disease 2019 (COVID-19) patients, and SARS-CoV-2 RNA has been detected in sewage. Thus, many regions worldwide have implemented the measurement of SARS-CoV-2 RNA in sewage as a monitoring tool for COVID-19 prevalence. Here, we report the monitoring of two wastewater treatment plants in Tokyo from May 2020 to June 2022 using a fully automated genetic testing method for the solid fraction of sewage. We found that SARS-CoV-2 sewage monitoring using the Panther System with the Aptima SARS-CoV-2 Assay Kit is an effective method of qualitative monitoring. Furthermore, spike gene mutations (N501Y, L452R, E484A) by real-time PCR using test residues were detected in some samples, which reflected the epidemic status. Nevertheless, our findings demonstrate that the use of a fully automated testing platform is effective for SARS-CoV-2 RNA detection in the solid fraction of sewage.
Keywords: COVID-19; SARS-CoV-2; Sewage surveillance; Tokyo.