tetano
Editor, Senior Moderator
Virus Res
. 2022 Jan 27;198701.
doi: 10.1016/j.virusres.2022.198701. Online ahead of print.
Rapid in vitro virucidal activity of slightly acidic hypochlorous acid water toward aerosolized coronavirus in simulated human-dispersed droplets
Yu Miyaoka[SUP] 1 [/SUP], Makiko Yamaguchi[SUP] 2 [/SUP], Chisaki Kadota[SUP] 2 [/SUP], Md Amirul Hasan[SUP] 1 [/SUP], Md Humayun Kabir[SUP] 1 [/SUP], Dany Shoham[SUP] 3 [/SUP], Harumi Murakami[SUP] 4 [/SUP], Kazuaki Takehara[SUP] 5 [/SUP]
Affiliations
Abstract
The virucidal activities were evaluated by spraying slightly acidic hypochlorous acid waters (SAHWs) containing various concentrations of free available chlorine - 100, 200, 300 and 500 ppm (SAHW-100, -200, -300 and -500, respectively) - toward aerosol of an avian coronavirus (infectious bronchitis virus: IBV). The viral solution was supplemented with 0.5% fetal bovine serum (FBS) to simulate normal human droplets generated by sneezing or coughing in a real-life scenario. The virus containing 0.5% FBS was sprayed and exposed to SAHWs for a few seconds in a closed chamber, before reaching the air sampler. The results showed that IBV exposed to SAHW-100 and -200 for a few seconds decreased by 0.21 log[SUB]10[/SUB] and 0.80 log[SUB]10[/SUB], respectively, compared to the pre-exposed samples to SAHWs as controls. On the other hand, reductions of 1.16 log[SUB]10[/SUB] and 1.67 log[SUB]10[/SUB] were achieved following the exposure to SAHW-300 and -500, respectively, within a few seconds. These results suggest that SAHWs have rapid in vitro virucidal activity toward aerosolized IBV. The findings obtained for IBV might basically be applicable in relation to SARS-CoV-2, given the resemblance between the two viruses. To prevent human-to-human transmissions by aerosols, the inactivation of viruses in the air by exposure to SAHWs for a few seconds seems to be an effective way.
Keywords: SAHW (slightly acidic hypochlorous acid water); aerosol; coronavirus; disinfection; infectious bronchitis virus; spray.
. 2022 Jan 27;198701.
doi: 10.1016/j.virusres.2022.198701. Online ahead of print.
Rapid in vitro virucidal activity of slightly acidic hypochlorous acid water toward aerosolized coronavirus in simulated human-dispersed droplets
Yu Miyaoka[SUP] 1 [/SUP], Makiko Yamaguchi[SUP] 2 [/SUP], Chisaki Kadota[SUP] 2 [/SUP], Md Amirul Hasan[SUP] 1 [/SUP], Md Humayun Kabir[SUP] 1 [/SUP], Dany Shoham[SUP] 3 [/SUP], Harumi Murakami[SUP] 4 [/SUP], Kazuaki Takehara[SUP] 5 [/SUP]
Affiliations
- PMID: 35093473
- DOI: 10.1016/j.virusres.2022.198701
Abstract
The virucidal activities were evaluated by spraying slightly acidic hypochlorous acid waters (SAHWs) containing various concentrations of free available chlorine - 100, 200, 300 and 500 ppm (SAHW-100, -200, -300 and -500, respectively) - toward aerosol of an avian coronavirus (infectious bronchitis virus: IBV). The viral solution was supplemented with 0.5% fetal bovine serum (FBS) to simulate normal human droplets generated by sneezing or coughing in a real-life scenario. The virus containing 0.5% FBS was sprayed and exposed to SAHWs for a few seconds in a closed chamber, before reaching the air sampler. The results showed that IBV exposed to SAHW-100 and -200 for a few seconds decreased by 0.21 log[SUB]10[/SUB] and 0.80 log[SUB]10[/SUB], respectively, compared to the pre-exposed samples to SAHWs as controls. On the other hand, reductions of 1.16 log[SUB]10[/SUB] and 1.67 log[SUB]10[/SUB] were achieved following the exposure to SAHW-300 and -500, respectively, within a few seconds. These results suggest that SAHWs have rapid in vitro virucidal activity toward aerosolized IBV. The findings obtained for IBV might basically be applicable in relation to SARS-CoV-2, given the resemblance between the two viruses. To prevent human-to-human transmissions by aerosols, the inactivation of viruses in the air by exposure to SAHWs for a few seconds seems to be an effective way.
Keywords: SAHW (slightly acidic hypochlorous acid water); aerosol; coronavirus; disinfection; infectious bronchitis virus; spray.