tetano
Editor, Senior Moderator
Virus Res
. 2021 Sep 13;198563.
doi: 10.1016/j.virusres.2021.198563. Online ahead of print.
Comparison of SARS-CoV-2 variant lethality in human angiotensin-converting enzyme 2 transgenic mice
Tae-Young Lee[SUP] 1 [/SUP], Hansaem Lee[SUP] 1 [/SUP], Nayoung Kim[SUP] 1 [/SUP], Pyeonghwa Jeon[SUP] 1 [/SUP], Jun-Won Kim[SUP] 1 [/SUP], Hee-Young Lim[SUP] 1 [/SUP], Jeong-Sun Yang[SUP] 1 [/SUP], Kyung-Chang Kim[SUP] 1 [/SUP], Joo-Yeon Lee[SUP] 2 [/SUP]
Affiliations
Abstract
This study compared the lethality of severe acute respiratory syndrome coronavirus 2 variants belonging to the S, V, L, G, GH, and GR clades using K18-human angiotensin-converting enzyme 2 heterozygous mice. To estimate the 50% lethal dose (LD[SUB]50[/SUB]) of each variant, increasing viral loads (10[SUP]0[/SUP]-10[SUP]4[/SUP] plaque-forming units [PFU]) were administered intranasally. Mouse weight and survival were monitored for 14 days. The LD[SUB]50[/SUB] of the GH and GR clades was significantly lower than that of other clades at 50 PFU. These findings suggest that the GH and GR clades, which are prevalent worldwide, are more virulent than the other clades.
Keywords: Genotype; K18-hACE2 transgenic mouse; Lethality; SARS-CoV-2.
. 2021 Sep 13;198563.
doi: 10.1016/j.virusres.2021.198563. Online ahead of print.
Comparison of SARS-CoV-2 variant lethality in human angiotensin-converting enzyme 2 transgenic mice
Tae-Young Lee[SUP] 1 [/SUP], Hansaem Lee[SUP] 1 [/SUP], Nayoung Kim[SUP] 1 [/SUP], Pyeonghwa Jeon[SUP] 1 [/SUP], Jun-Won Kim[SUP] 1 [/SUP], Hee-Young Lim[SUP] 1 [/SUP], Jeong-Sun Yang[SUP] 1 [/SUP], Kyung-Chang Kim[SUP] 1 [/SUP], Joo-Yeon Lee[SUP] 2 [/SUP]
Affiliations
- PMID: 34530046
- PMCID: PMC8437746
- DOI: 10.1016/j.virusres.2021.198563
Abstract
This study compared the lethality of severe acute respiratory syndrome coronavirus 2 variants belonging to the S, V, L, G, GH, and GR clades using K18-human angiotensin-converting enzyme 2 heterozygous mice. To estimate the 50% lethal dose (LD[SUB]50[/SUB]) of each variant, increasing viral loads (10[SUP]0[/SUP]-10[SUP]4[/SUP] plaque-forming units [PFU]) were administered intranasally. Mouse weight and survival were monitored for 14 days. The LD[SUB]50[/SUB] of the GH and GR clades was significantly lower than that of other clades at 50 PFU. These findings suggest that the GH and GR clades, which are prevalent worldwide, are more virulent than the other clades.
Keywords: Genotype; K18-hACE2 transgenic mouse; Lethality; SARS-CoV-2.