tetano
Editor, Senior Moderator
Virus Res
. 2022 Apr 13;198786.
doi: 10.1016/j.virusres.2022.198786. Online ahead of print.
Comparative analysis of SARS-CoV-2 quasispecies in the upper and lower respiratory tract shows an ongoing evolution in the spike cleavage site
Stefano Gaiarsa[SUP] 1 [/SUP], Federica Giardina[SUP] 1 [/SUP], Gherard Batisti Biffignandi[SUP] 2 [/SUP], Guglielmo Ferrari[SUP] 1 [/SUP], Aurora Piazza[SUP] 2 [/SUP], Monica Tallarita[SUP] 1 [/SUP], Federica Novazzi[SUP] 1 [/SUP], Claudio Bandi[SUP] 3 [/SUP], Stefania Paolucci[SUP] 1 [/SUP], Francesca Rovida[SUP] 4 [/SUP], Giulia Campanini[SUP] 1 [/SUP], Antonio Piralla[SUP] 5 [/SUP], Fausto Baldanti[SUP] 4 [/SUP]
Affiliations
Abstract
Studies are needed to better understand the genomic evolution of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This study aimed to describe viral quasispecies population of upper and lower respiratory tract by next-generation sequencing in patients admitted to intensive care unit. A deep sequencing of the S gene of SARS-CoV-2 from 109 clinical specimens, sampled from the upper respiratory tract (URT) and lower respiratory tract (LRT) of 77 patients was performed. A higher incidence of non-synonymous mutations and indels was observed in the LRT among minority variants. This might be explained by the ability of the virus to invade cells without interacting with ACE2 (e.g. exploiting macrophage phagocytosis). Minority variants are highly concentrated around the gene portion encoding for the Spike cleavage site, with a higher incidence in the URT; four mutations are highly recurring among samples and were found associated with the URT. Interestingly, 55.8% of minority variants detected in this locus were T>G and G>T transversions. Results from this study evidenced the presence of selective pressure and suggest that an evolutionary process is still ongoing in one of the crucial sites of spike protein associated with the spillover to humans.
Keywords: SARS-CoV-2; Spike protein; deep sequencing; deleterious mutations; minority variants; spillover.
. 2022 Apr 13;198786.
doi: 10.1016/j.virusres.2022.198786. Online ahead of print.
Comparative analysis of SARS-CoV-2 quasispecies in the upper and lower respiratory tract shows an ongoing evolution in the spike cleavage site
Stefano Gaiarsa[SUP] 1 [/SUP], Federica Giardina[SUP] 1 [/SUP], Gherard Batisti Biffignandi[SUP] 2 [/SUP], Guglielmo Ferrari[SUP] 1 [/SUP], Aurora Piazza[SUP] 2 [/SUP], Monica Tallarita[SUP] 1 [/SUP], Federica Novazzi[SUP] 1 [/SUP], Claudio Bandi[SUP] 3 [/SUP], Stefania Paolucci[SUP] 1 [/SUP], Francesca Rovida[SUP] 4 [/SUP], Giulia Campanini[SUP] 1 [/SUP], Antonio Piralla[SUP] 5 [/SUP], Fausto Baldanti[SUP] 4 [/SUP]
Affiliations
- PMID: 35429618
- DOI: 10.1016/j.virusres.2022.198786
Abstract
Studies are needed to better understand the genomic evolution of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This study aimed to describe viral quasispecies population of upper and lower respiratory tract by next-generation sequencing in patients admitted to intensive care unit. A deep sequencing of the S gene of SARS-CoV-2 from 109 clinical specimens, sampled from the upper respiratory tract (URT) and lower respiratory tract (LRT) of 77 patients was performed. A higher incidence of non-synonymous mutations and indels was observed in the LRT among minority variants. This might be explained by the ability of the virus to invade cells without interacting with ACE2 (e.g. exploiting macrophage phagocytosis). Minority variants are highly concentrated around the gene portion encoding for the Spike cleavage site, with a higher incidence in the URT; four mutations are highly recurring among samples and were found associated with the URT. Interestingly, 55.8% of minority variants detected in this locus were T>G and G>T transversions. Results from this study evidenced the presence of selective pressure and suggest that an evolutionary process is still ongoing in one of the crucial sites of spike protein associated with the spillover to humans.
Keywords: SARS-CoV-2; Spike protein; deep sequencing; deleterious mutations; minority variants; spillover.