tetano
Editor, Senior Moderator
Virus Genes
. 2023 Jul 3.
doi: 10.1007/s11262-023-01996-y. Online ahead of print. Screening and identification of specific cluster miRNAs in N2a cells infected by H7N9 virus
Yitong Yin[SUP] 1 [/SUP], Zengzhao Qiu[SUP] 2 [/SUP], Yuxuan Lei[SUP] 2 [/SUP], Jia Huang[SUP] 3 [/SUP], Ying Sun[SUP] 4 [/SUP], Hui Liu[SUP] 4 [/SUP], Weihua Wu[SUP] 4 [/SUP], Xin Wang[SUP] 4 [/SUP], Yuelong Shu[SUP] 2 5 [/SUP], Qing Zheng[SUP] 6 [/SUP], Shisong Fang[SUP] 7 [/SUP]
Affiliations
This study aims to screen and identify specific cluster miRNAs of H7N9 virus-infected N2a cells and explore the possible pathogenesis of these miRNAs. The N2a cells are infected with H7N9 and H1N1 influenza viruses, and the cells are collected at 12, 24 and 48 h to extract total RNA. To sequence miRNAs and identify different virus-specific miRNAs, high-throughput sequencing technology is used. Fifteen H7N9 virus-specific cluster miRNAs are screened, and eight of them are included in the miRBase database. These cluster-specific miRNAs regulate many signaling pathways, such as the PI3K-Akt signaling pathway, the RAS signaling pathway, the cAMP signaling pathway, actin cytoskeleton regulation and cancer-related genes. The study provides a scientific basis for the pathogenesis of H7N9 avian influenza, which is regulated by miRNAs.
Keywords: H1N1 virus; H7N9 virus; Neuroblastoma cell (N2a); microRNA.
. 2023 Jul 3.
doi: 10.1007/s11262-023-01996-y. Online ahead of print. Screening and identification of specific cluster miRNAs in N2a cells infected by H7N9 virus
Yitong Yin[SUP] 1 [/SUP], Zengzhao Qiu[SUP] 2 [/SUP], Yuxuan Lei[SUP] 2 [/SUP], Jia Huang[SUP] 3 [/SUP], Ying Sun[SUP] 4 [/SUP], Hui Liu[SUP] 4 [/SUP], Weihua Wu[SUP] 4 [/SUP], Xin Wang[SUP] 4 [/SUP], Yuelong Shu[SUP] 2 5 [/SUP], Qing Zheng[SUP] 6 [/SUP], Shisong Fang[SUP] 7 [/SUP]
Affiliations
- PMID: 37395889
- DOI: 10.1007/s11262-023-01996-y
This study aims to screen and identify specific cluster miRNAs of H7N9 virus-infected N2a cells and explore the possible pathogenesis of these miRNAs. The N2a cells are infected with H7N9 and H1N1 influenza viruses, and the cells are collected at 12, 24 and 48 h to extract total RNA. To sequence miRNAs and identify different virus-specific miRNAs, high-throughput sequencing technology is used. Fifteen H7N9 virus-specific cluster miRNAs are screened, and eight of them are included in the miRBase database. These cluster-specific miRNAs regulate many signaling pathways, such as the PI3K-Akt signaling pathway, the RAS signaling pathway, the cAMP signaling pathway, actin cytoskeleton regulation and cancer-related genes. The study provides a scientific basis for the pathogenesis of H7N9 avian influenza, which is regulated by miRNAs.
Keywords: H1N1 virus; H7N9 virus; Neuroblastoma cell (N2a); microRNA.