tetano
Editor, Senior Moderator
Virus Evol
. 2021 Jun 4;7(1):veab047.
doi: 10.1093/ve/veab047. eCollection 2021 Jan.
Identification of H3N2 NA and PB1-F2 genetic variants and their association with disease symptoms during the 2014-15 influenza season
Deena R Blumenkrantz[SUP] 1 [/SUP], Thomas Mehoke[SUP] 2 [/SUP], Kathryn Shaw-Saliba[SUP] 1 3 [/SUP], Harrison Powell[SUP] 1 [/SUP], Nicholas Wohlgemuth[SUP] 1 [/SUP], Hsuan Liu[SUP] 1 [/SUP], Elizabeth Macias[SUP] 4 [/SUP], Jared Evans[SUP] 2 [/SUP], Mitra Lewis[SUP] 5 [/SUP], Rebecca Medina[SUP] 5 [/SUP], Justin Hardick[SUP] 5 [/SUP], Lauren M Sauer[SUP] 5 [/SUP], Andrea Dugas[SUP] 5 [/SUP], Anna DuVal[SUP] 3 [/SUP], Andrew P Lane[SUP] 6 [/SUP], Charlotte Gaydos[SUP] 3 5 [/SUP], Richard Rothman[SUP] 3 5 [/SUP], Peter Thielen[SUP] 2 [/SUP], Andrew Pekosz[SUP] 1 [/SUP]
Affiliations
Abstract
The 2014-15 influenza season saw the emergence of an H3N2 antigenic drift variant that formed the 3C.2a HA clade. Whole viral genomes were sequenced from nasopharyngeal swabs of ninety-four patients with confirmed influenza A virus infection and primary human nasal epithelial cell cultures used to efficiently isolate H3N2 viruses. The isolates were classified by HA clade and the presence of a new set of co-selected mutations in NA (a glycosylation site, NAg+) and PB1-F2 (H75P). The NA and PB1-F2 mutations were present in a subset of clade 3C.2a viruses (NAg+F2P), which dominated during the subsequent influenza seasons. In human nasal epithelial cell cultures, a virus with the novel NAg+F2P genotype replicated less well compared with a virus with the parental genotype. Retrospective analyses of clinical data showed that NAg+F2P genotype viruses were associated with increased cough and shortness of breath in infected patients.
Keywords: H3N2; NA; PB1-F2; antigenic drift; human; influenza; symptoms.
. 2021 Jun 4;7(1):veab047.
doi: 10.1093/ve/veab047. eCollection 2021 Jan.
Identification of H3N2 NA and PB1-F2 genetic variants and their association with disease symptoms during the 2014-15 influenza season
Deena R Blumenkrantz[SUP] 1 [/SUP], Thomas Mehoke[SUP] 2 [/SUP], Kathryn Shaw-Saliba[SUP] 1 3 [/SUP], Harrison Powell[SUP] 1 [/SUP], Nicholas Wohlgemuth[SUP] 1 [/SUP], Hsuan Liu[SUP] 1 [/SUP], Elizabeth Macias[SUP] 4 [/SUP], Jared Evans[SUP] 2 [/SUP], Mitra Lewis[SUP] 5 [/SUP], Rebecca Medina[SUP] 5 [/SUP], Justin Hardick[SUP] 5 [/SUP], Lauren M Sauer[SUP] 5 [/SUP], Andrea Dugas[SUP] 5 [/SUP], Anna DuVal[SUP] 3 [/SUP], Andrew P Lane[SUP] 6 [/SUP], Charlotte Gaydos[SUP] 3 5 [/SUP], Richard Rothman[SUP] 3 5 [/SUP], Peter Thielen[SUP] 2 [/SUP], Andrew Pekosz[SUP] 1 [/SUP]
Affiliations
- PMID: 34131512
- PMCID: PMC8197029
- DOI: 10.1093/ve/veab047
Abstract
The 2014-15 influenza season saw the emergence of an H3N2 antigenic drift variant that formed the 3C.2a HA clade. Whole viral genomes were sequenced from nasopharyngeal swabs of ninety-four patients with confirmed influenza A virus infection and primary human nasal epithelial cell cultures used to efficiently isolate H3N2 viruses. The isolates were classified by HA clade and the presence of a new set of co-selected mutations in NA (a glycosylation site, NAg+) and PB1-F2 (H75P). The NA and PB1-F2 mutations were present in a subset of clade 3C.2a viruses (NAg+F2P), which dominated during the subsequent influenza seasons. In human nasal epithelial cell cultures, a virus with the novel NAg+F2P genotype replicated less well compared with a virus with the parental genotype. Retrospective analyses of clinical data showed that NAg+F2P genotype viruses were associated with increased cough and shortness of breath in infected patients.
Keywords: H3N2; NA; PB1-F2; antigenic drift; human; influenza; symptoms.