tetano
Editor, Senior Moderator
Virulence
. 2022 Dec;13(1):355-369.
doi: 10.1080/21505594.2022.2032928.
MERS-CoV nsp1 impairs the cellular metabolic processes by selectively downregulating mRNAs in a novel granules
Zhaoyi Pan[SUP] 1 [/SUP], Yujie Feng[SUP] 1 [/SUP], Zhihui Wang[SUP] 1 [/SUP], Zhengyang Lei[SUP] 1 [/SUP], Qiuju Han[SUP] 1 [/SUP], Jian Zhang[SUP] 1 [/SUP]
Affiliations
Abstract
MERS-CoV infection can damage the cellular metabolic processes, but the underlying mechanisms are largely unknown. Through screening, we found non-structural protein 1 (nsp1) of MERS-CoV could inhibit cell viability, cell cycle, and cell migration through its endonuclease activity. Transcriptome sequencing revealed that MERS-CoV nsp1 specifically downregulated the mRNAs of ribosomal protein genes, oxidative phosphorylation protein genes, and antigen presentation genes, but upregulated the mRNAs of transcriptional regulatory genes. Further analysis shown nsp1 existed in a novel ribonucleosome complex formed via liquid-liquid phase separation, which did not co-localize with mitochondria, lysosomes, P-bodies, or stress granules. Interestingly, the nsp1-located granules specifically contained mRNAs of ribosomal protein genes and oxidative phosphorylation genes, which may explain why MERS-CoV nsp1 selectively degraded these mRNAs in cells. Finally, MERS-CoV nsp1 transgenic mice showed significant loss of body weight and an increased sensitivity to poly(I:C)-induced inflammatory death. These findings demonstrate a new mechanism by which MERS-CoV impairs cell viability, which serves as a potential novel target for preventing MERS-CoV infection-induced pathological damage.Abbreviations: (Middle East respiratory syndrome coronavirus (MERS-CoV), Actinomycin D (Act D), liquid-liquid phase separation (LLPS), stress granules (SGs), Mass spectrometry (IP-MS), RNA Binding Protein Immunoprecipitation (RIP)).
Keywords: LLPS; MERS-CoV; nsp1; oxidative phosphorylation; ribosome.
. 2022 Dec;13(1):355-369.
doi: 10.1080/21505594.2022.2032928.
MERS-CoV nsp1 impairs the cellular metabolic processes by selectively downregulating mRNAs in a novel granules
Zhaoyi Pan[SUP] 1 [/SUP], Yujie Feng[SUP] 1 [/SUP], Zhihui Wang[SUP] 1 [/SUP], Zhengyang Lei[SUP] 1 [/SUP], Qiuju Han[SUP] 1 [/SUP], Jian Zhang[SUP] 1 [/SUP]
Affiliations
- PMID: 35129074
- DOI: 10.1080/21505594.2022.2032928
Abstract
MERS-CoV infection can damage the cellular metabolic processes, but the underlying mechanisms are largely unknown. Through screening, we found non-structural protein 1 (nsp1) of MERS-CoV could inhibit cell viability, cell cycle, and cell migration through its endonuclease activity. Transcriptome sequencing revealed that MERS-CoV nsp1 specifically downregulated the mRNAs of ribosomal protein genes, oxidative phosphorylation protein genes, and antigen presentation genes, but upregulated the mRNAs of transcriptional regulatory genes. Further analysis shown nsp1 existed in a novel ribonucleosome complex formed via liquid-liquid phase separation, which did not co-localize with mitochondria, lysosomes, P-bodies, or stress granules. Interestingly, the nsp1-located granules specifically contained mRNAs of ribosomal protein genes and oxidative phosphorylation genes, which may explain why MERS-CoV nsp1 selectively degraded these mRNAs in cells. Finally, MERS-CoV nsp1 transgenic mice showed significant loss of body weight and an increased sensitivity to poly(I:C)-induced inflammatory death. These findings demonstrate a new mechanism by which MERS-CoV impairs cell viability, which serves as a potential novel target for preventing MERS-CoV infection-induced pathological damage.Abbreviations: (Middle East respiratory syndrome coronavirus (MERS-CoV), Actinomycin D (Act D), liquid-liquid phase separation (LLPS), stress granules (SGs), Mass spectrometry (IP-MS), RNA Binding Protein Immunoprecipitation (RIP)).
Keywords: LLPS; MERS-CoV; nsp1; oxidative phosphorylation; ribosome.