tetano
Editor, Senior Moderator
Virology
Available online 6 July 2012
In Press, Corrected Proof ? Note to users
Cover image
The influenza fingerprints: NS1 and M1 proteins contribute to specific host cell ultrastructure signatures upon infection by different influenza A viruses
Olivier Terriera, 1,
Vincent Moulesa, 1,
Coralie Carrona,
Ga?lle Carteta,
Emilie Frobertb,
Matthieu Yvera,
Aurelien Traversiera,
Thorsten Wolffc,
Beatrice Riteaud,
Nadia Naffakhe,
Bruno Linab, d,
Jean-Jacques Diazf,
Manuel Rosa-Calatravaa, Corresponding author contact information, E-mail the corresponding author
a Equipe VirCell, Laboratoire de Virologie et Pathologie Humaine, VirPath EMR 4610, Universit? de Lyon, Universit? Claude Bernard Lyon 1, Hospices Civils de Lyon, Facult? de m?decine RTH Laennec, rue Guillaume Paradin, F-69008 Lyon, France
b Laboratoire de Virologie, Centre de Biologie et de Pathologie Est, Hospices Civils de Lyon, 59 boulevard Pinel, F-69677 Bron Cedex, Lyon, France
c Division of Influenza/Respiratory Viruses, Robert Koch Institute, Nordufer 20, D-13353 Berlin, Germany
d Laboratoire de Virologie et Pathologie Humaine, VirPath EMR 4610, Universit? de Lyon, Universit? Claude Bernard Lyon 1, Hospices Civils de Lyon, Facult? de m?decine RTH Laennec, rue Guillaume Paradin, F-69008 Lyon, France
e Institut Pasteur, Unit? de G?n?tique Mol?culaire des Virus Respiratoires, URA CNRS 3015, EA302 Universit? Paris Diderot, Paris, France
f Centre de Recherche en Canc?rologie de Lyon UMR Inserm 1052 CNRS 5286, Centre L?on B?rard, FNCLCC, F-69373 Lyon, France
Available online 6 July 2012.
http://dx.doi.org/10.1016/j.virol.2012.05.019, How to Cite or Link Using DOI
Abstract
Influenza A are nuclear replicating viruses which hijack host machineries in order to achieve optimal infection. Numerous functional virus?host interactions have now been characterized, but little information has been gathered concerning their link to the virally induced remodeling of the host cellular architecture. In this study, we infected cells with several human and avian influenza viruses and we have analyzed their ultrastructural modifications by using electron and confocal microscopy. We discovered that infections lead to a major and systematic disruption of nucleoli and the formation of a large number of diverse viral structures showing specificity that depended on the subtype origin and genomic composition of viruses. We identified NS1 and M1 proteins as the main actors in the remodeling of the host ultra-structure and our results suggest that each influenza A virus strain could be associated with a specific cellular fingerprint, possibly correlated to the functional properties of their viral components.
http://0-www.sciencedirect.com.precise.petronas.com.my/science/article/pii/S004268221200270X
Available online 6 July 2012
In Press, Corrected Proof ? Note to users
Cover image
The influenza fingerprints: NS1 and M1 proteins contribute to specific host cell ultrastructure signatures upon infection by different influenza A viruses
Olivier Terriera, 1,
Vincent Moulesa, 1,
Coralie Carrona,
Ga?lle Carteta,
Emilie Frobertb,
Matthieu Yvera,
Aurelien Traversiera,
Thorsten Wolffc,
Beatrice Riteaud,
Nadia Naffakhe,
Bruno Linab, d,
Jean-Jacques Diazf,
Manuel Rosa-Calatravaa, Corresponding author contact information, E-mail the corresponding author
a Equipe VirCell, Laboratoire de Virologie et Pathologie Humaine, VirPath EMR 4610, Universit? de Lyon, Universit? Claude Bernard Lyon 1, Hospices Civils de Lyon, Facult? de m?decine RTH Laennec, rue Guillaume Paradin, F-69008 Lyon, France
b Laboratoire de Virologie, Centre de Biologie et de Pathologie Est, Hospices Civils de Lyon, 59 boulevard Pinel, F-69677 Bron Cedex, Lyon, France
c Division of Influenza/Respiratory Viruses, Robert Koch Institute, Nordufer 20, D-13353 Berlin, Germany
d Laboratoire de Virologie et Pathologie Humaine, VirPath EMR 4610, Universit? de Lyon, Universit? Claude Bernard Lyon 1, Hospices Civils de Lyon, Facult? de m?decine RTH Laennec, rue Guillaume Paradin, F-69008 Lyon, France
e Institut Pasteur, Unit? de G?n?tique Mol?culaire des Virus Respiratoires, URA CNRS 3015, EA302 Universit? Paris Diderot, Paris, France
f Centre de Recherche en Canc?rologie de Lyon UMR Inserm 1052 CNRS 5286, Centre L?on B?rard, FNCLCC, F-69373 Lyon, France
Available online 6 July 2012.
http://dx.doi.org/10.1016/j.virol.2012.05.019, How to Cite or Link Using DOI
Abstract
Influenza A are nuclear replicating viruses which hijack host machineries in order to achieve optimal infection. Numerous functional virus?host interactions have now been characterized, but little information has been gathered concerning their link to the virally induced remodeling of the host cellular architecture. In this study, we infected cells with several human and avian influenza viruses and we have analyzed their ultrastructural modifications by using electron and confocal microscopy. We discovered that infections lead to a major and systematic disruption of nucleoli and the formation of a large number of diverse viral structures showing specificity that depended on the subtype origin and genomic composition of viruses. We identified NS1 and M1 proteins as the main actors in the remodeling of the host ultra-structure and our results suggest that each influenza A virus strain could be associated with a specific cellular fingerprint, possibly correlated to the functional properties of their viral components.
http://0-www.sciencedirect.com.precise.petronas.com.my/science/article/pii/S004268221200270X