Giuseppe
Emeritus
Virology. 2014 454-455:197-205 - DOI: 10.1016/j.virol.2014.02.018 - PMID: 24725946
Productive replication of Middle East respiratory syndrome coronavirus in monocyte-derived dendritic cells modulates innate immune response.
Chu, Hin; Zhou, Jie; Ho-Yin Wong, Bosco; Li, Cun; Cheng, Zhong-Shan S; Lin, Xiang; Kwok-Man Poon, Vincent; Sun, Tianhao; Choi-Yi Lau, Candy; Fuk-Woo Chan, Jasper; Kai-Wang To, Kelvin; Chan, Kwok-**** H; Lu, Liwei; Zheng, Bo-Jian J; Yuen, Kwok-Yung Y
Abstract
The Middle East respiratory syndrome coronavirus (MERS-CoV) closely resembled severe acute respiratory syndrome coronavirus (SARS-CoV) in disease manifestation as rapidly progressive acute pneumonia with multi-organ dysfunction. Using monocyte-derived-dendritic cells (Mo-DCs), we discovered fundamental discrepancies in the outcome of MERS-CoV- and SARS-CoV-infection. First, MERS-CoV productively infected Mo-DCs while SARS-CoV-infection was abortive. Second, MERS-CoV induced significantly higher levels of IFN-γ, IP-10, IL-12, and RANTES expression than SARS-CoV. Third, MERS-CoV-infection induced higher surface expression of MHC class II (HLA-DR) and the co-stimulatory molecule CD86 than SARS-CoV-infection. Overall, our data suggests that the dendritic cell can serve as an important target of viral replication and a vehicle for dissemination. MERS-CoV-infection in DCs results in the production of a rich combination of cytokines and chemokines, and modulates innate immune response differently from that of SARS-CoV-infection. Our findings may help to explain the apparent discrepancy in the pathogenicity between MERS-CoV and SARS-CoV.
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Productive replication of Middle East respiratory syndrome coronavirus in monocyte-derived dendritic cells modulates innate immune response.
Chu, Hin; Zhou, Jie; Ho-Yin Wong, Bosco; Li, Cun; Cheng, Zhong-Shan S; Lin, Xiang; Kwok-Man Poon, Vincent; Sun, Tianhao; Choi-Yi Lau, Candy; Fuk-Woo Chan, Jasper; Kai-Wang To, Kelvin; Chan, Kwok-**** H; Lu, Liwei; Zheng, Bo-Jian J; Yuen, Kwok-Yung Y
Abstract
The Middle East respiratory syndrome coronavirus (MERS-CoV) closely resembled severe acute respiratory syndrome coronavirus (SARS-CoV) in disease manifestation as rapidly progressive acute pneumonia with multi-organ dysfunction. Using monocyte-derived-dendritic cells (Mo-DCs), we discovered fundamental discrepancies in the outcome of MERS-CoV- and SARS-CoV-infection. First, MERS-CoV productively infected Mo-DCs while SARS-CoV-infection was abortive. Second, MERS-CoV induced significantly higher levels of IFN-γ, IP-10, IL-12, and RANTES expression than SARS-CoV. Third, MERS-CoV-infection induced higher surface expression of MHC class II (HLA-DR) and the co-stimulatory molecule CD86 than SARS-CoV-infection. Overall, our data suggests that the dendritic cell can serve as an important target of viral replication and a vehicle for dissemination. MERS-CoV-infection in DCs results in the production of a rich combination of cytokines and chemokines, and modulates innate immune response differently from that of SARS-CoV-infection. Our findings may help to explain the apparent discrepancy in the pathogenicity between MERS-CoV and SARS-CoV.
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