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Virology . Pathogenicity and transmission of novel highly pathogenic H7N2 variants originating from H7N9 avian influenza viruses in chickens

tetano

Editor, Senior Moderator
Virology


. 2024 May 23:597:110121.
doi: 10.1016/j.virol.2024.110121. Online ahead of print. Pathogenicity and transmission of novel highly pathogenic H7N2 variants originating from H7N9 avian influenza viruses in chickens

Jinyu Huang[SUP] 1 [/SUP], Kaixiong Ma[SUP] 2 [/SUP], Jiahao Zhang[SUP] 2 [/SUP], Jiangtao Zhou[SUP] 1 [/SUP], Jiahui Yi[SUP] 1 [/SUP], Wenbao Qi[SUP] 3 [/SUP], Ming Liao[SUP] 4 [/SUP]



Affiliations
Abstract

The H7 subtype avian influenza viruses are circulating widely worldwide, causing significant economic losses to the poultry industry and posing a serious threat to human health. In 2019, H7N2 and H7N9 co-circulated in Chinese poultry, yet the risk of H7N2 remained unclear. We isolated and sequenced four H7N2 viruses from chickens, revealing them as novel reassortants with H7N9-derived HA, M, NS genes and H9N2-derived PB2, PB1, PA,NP, NA genes. To further explore the key segment of pathogenicity, H7N2-H7N9NA and H7N2-H9N2HA single-substitution were constructed. Pathogenicity study showed H7N2 isolates to be highly pathogenic in chickens, with H7N2-H7N9NA slightly weaker than H7N2-Wild type. Transcriptomic analysis suggested that H7N9-derived HA genes primarily drove the high pathogenicity of H7N2 isolates, eliciting a strong inflammatory response. These findings underscored the increased threat posed by reassorted H7N2 viruses to chickens, emphasizing the necessity of long-term monitoring of H7 subtype avian influenza viruses.

Keywords: Avian influenza virus; H7N2; H7N9; Pathogenicity; Reassortment.

 
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