tetano
Editor, Senior Moderator
Virology
. 2023 Nov 2:589:109920.
doi: 10.1016/j.virol.2023.109920. Online ahead of print. Identification of subgenomic mRNAs derived from the coronavirus 1a/1b protein gene: Implications for coronavirus transcription
Ching-Hung Lin[SUP] 1 [/SUP], Feng-Cheng Hsieh[SUP] 1 [/SUP], Meilin Wang[SUP] 2 [/SUP], Chieh Hsu[SUP] 1 [/SUP], Hsuan-Wei Hsu[SUP] 1 [/SUP], Chun-Chun Yang[SUP] 1 [/SUP], Cheng-Yao Yang[SUP] 3 [/SUP], Hung-Yi Wu[SUP] 4 [/SUP]
Affiliations
Synthesis of coronavirus subgenomic mRNA (sgmRNA) is guided by the transcription regulatory sequence (TRS). sgmRNA derived from the body TRS (TRS-B) located at the 1a/1b protein gene is designated 1ab/sgmRNA. In the current study, we comprehensively identified the 1ab/sgmRNAs synthesized from TRS-Bs located at the 1a/1b protein genes of different coronavirus genera both in vitro and in vivo by RT‒PCR and sequencing. The results suggested that the degree of sequence homology between the leader TRS (TRS-L) and TRS-B may not be a decisive factor for 1ab/sgmRNA synthesis. This observation led us to revisit the coronavirus transcription mechanism and to propose that the disassociation of coronavirus polymerase from the viral genome may be a prerequisite for sgmRNA synthesis. Once the polymerase can disassociate at TRS-B, the sequence homology between TRS-L and TRS-B is important for sgmRNA synthesis. The study therefore extends our understanding of transcription mechanisms.
Keywords: Coronavirus; Coronavirus genome structure; Coronavirus transcription; Gene expression; Subgenomic mRNA; Transcription regulatory sequence.
. 2023 Nov 2:589:109920.
doi: 10.1016/j.virol.2023.109920. Online ahead of print. Identification of subgenomic mRNAs derived from the coronavirus 1a/1b protein gene: Implications for coronavirus transcription
Ching-Hung Lin[SUP] 1 [/SUP], Feng-Cheng Hsieh[SUP] 1 [/SUP], Meilin Wang[SUP] 2 [/SUP], Chieh Hsu[SUP] 1 [/SUP], Hsuan-Wei Hsu[SUP] 1 [/SUP], Chun-Chun Yang[SUP] 1 [/SUP], Cheng-Yao Yang[SUP] 3 [/SUP], Hung-Yi Wu[SUP] 4 [/SUP]
Affiliations
- PMID: 37952466
- DOI: 10.1016/j.virol.2023.109920
Synthesis of coronavirus subgenomic mRNA (sgmRNA) is guided by the transcription regulatory sequence (TRS). sgmRNA derived from the body TRS (TRS-B) located at the 1a/1b protein gene is designated 1ab/sgmRNA. In the current study, we comprehensively identified the 1ab/sgmRNAs synthesized from TRS-Bs located at the 1a/1b protein genes of different coronavirus genera both in vitro and in vivo by RT‒PCR and sequencing. The results suggested that the degree of sequence homology between the leader TRS (TRS-L) and TRS-B may not be a decisive factor for 1ab/sgmRNA synthesis. This observation led us to revisit the coronavirus transcription mechanism and to propose that the disassociation of coronavirus polymerase from the viral genome may be a prerequisite for sgmRNA synthesis. Once the polymerase can disassociate at TRS-B, the sequence homology between TRS-L and TRS-B is important for sgmRNA synthesis. The study therefore extends our understanding of transcription mechanisms.
Keywords: Coronavirus; Coronavirus genome structure; Coronavirus transcription; Gene expression; Subgenomic mRNA; Transcription regulatory sequence.