tetano
Editor, Senior Moderator
Virology
. 2022 Feb 24;569:44-55.
doi: 10.1016/j.virol.2022.02.004. Online ahead of print.
Functional antibody-dependent cell mediated cytotoxicity (ADCC) responses to vaccine and circulating influenza strains following vaccination
Xuemin Chen[SUP] 1 [/SUP], He-Ying Sun[SUP] 2 [/SUP], Chun Yi Lee[SUP] 3 [/SUP], Christina A Rostad[SUP] 4 [/SUP], Jessica Trost[SUP] 5 [/SUP], Rodrigo B Abreu[SUP] 6 [/SUP], Michael A Carlock[SUP] 6 [/SUP], Jason R Wilson[SUP] 7 [/SUP], Shane Gansebom[SUP] 8 [/SUP], Ted M Ross[SUP] 9 [/SUP], David A Steinhauer[SUP] 10 [/SUP], Evan J Anderson[SUP] 11 [/SUP], Larry J Anderson[SUP] 12 [/SUP]
Affiliations
Abstract
Novel cell-based assays were developed to assess antibody-dependence cellular cytotoxicity (ADCC) antibodies against both vaccine and a representative circulation strain HA and NA proteins for the 2014-15 influenza season. The four assays using target cells stably expressing one of the four proteins worked well. In pre- and post-vaccine sera from 70 participants in a pre-season vaccine trial, we found ADCC antibodies and a rise in ADCC antibody titer against target cells expressing the 4 proteins but a much higher titer for the vaccine than the circulating HA in both pre-and post-vaccine sera. These differences in HA ADCC antibodies were not reflected in differences in HA binding antibodies. Our observations suggested that relatively minor changes on the subtype HA can result in large differences in ADCC activity.
Keywords: Antibody-dependent cellular cytotoxicity (ADCC); Influenza hemagglutinin (HA); Influenza neuraminidase (NA); Influenza virus vaccine.
. 2022 Feb 24;569:44-55.
doi: 10.1016/j.virol.2022.02.004. Online ahead of print.
Functional antibody-dependent cell mediated cytotoxicity (ADCC) responses to vaccine and circulating influenza strains following vaccination
Xuemin Chen[SUP] 1 [/SUP], He-Ying Sun[SUP] 2 [/SUP], Chun Yi Lee[SUP] 3 [/SUP], Christina A Rostad[SUP] 4 [/SUP], Jessica Trost[SUP] 5 [/SUP], Rodrigo B Abreu[SUP] 6 [/SUP], Michael A Carlock[SUP] 6 [/SUP], Jason R Wilson[SUP] 7 [/SUP], Shane Gansebom[SUP] 8 [/SUP], Ted M Ross[SUP] 9 [/SUP], David A Steinhauer[SUP] 10 [/SUP], Evan J Anderson[SUP] 11 [/SUP], Larry J Anderson[SUP] 12 [/SUP]
Affiliations
- PMID: 35255298
- DOI: 10.1016/j.virol.2022.02.004
Abstract
Novel cell-based assays were developed to assess antibody-dependence cellular cytotoxicity (ADCC) antibodies against both vaccine and a representative circulation strain HA and NA proteins for the 2014-15 influenza season. The four assays using target cells stably expressing one of the four proteins worked well. In pre- and post-vaccine sera from 70 participants in a pre-season vaccine trial, we found ADCC antibodies and a rise in ADCC antibody titer against target cells expressing the 4 proteins but a much higher titer for the vaccine than the circulating HA in both pre-and post-vaccine sera. These differences in HA ADCC antibodies were not reflected in differences in HA binding antibodies. Our observations suggested that relatively minor changes on the subtype HA can result in large differences in ADCC activity.
Keywords: Antibody-dependent cellular cytotoxicity (ADCC); Influenza hemagglutinin (HA); Influenza neuraminidase (NA); Influenza virus vaccine.