tetano
Editor, Senior Moderator
Clin Microbiol Infect. 2016 Feb 29. pii: S1198-743X(16)00115-4. doi: 10.1016/j.cmi.2016.02.007. [Epub ahead of print]
[h=1]Virological surveillance of influenza and other respiratory viruses during six consecutive seasons from 2006 to 2012 in Catalonia, Spain.[/h] Anton A[SUP]1[/SUP], Marcos MA[SUP]2[/SUP], Torner N[SUP]3[/SUP], Isanta R[SUP]2[/SUP], Camps M[SUP]2[/SUP], Martinez A[SUP]3[/SUP], Dom?nguez A[SUP]3[/SUP], Jan? M[SUP]4[/SUP], Jim?nez de Anta MT[SUP]2[/SUP], Pumarola T[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Main attention is given to seasonal influenza and respiratory syncytial viruses outbreaks, but the cumulative burden caused by other respiratory viruses (RV) is not widely considered. The aim of the present study is to describe the RV circulation in the general population during six consecutive seasons from 2006 to 2012 in Catalonia, Spain. Cell culture, immunofluorescence and PCR-based assays were used for the RV laboratory-confirmation and subtyping. Phylogenetic and molecular characterisations of viral HA, NA and M2 were performed from a representative sampling of influenza viruses. A total of 6,315 nasopharyngeal samples were collected, of which the 64% were laboratory-confirmed, mainly by influenza A viruses and rhinoviruses. Results show the significant burden of viral aetiological agents in acute respiratory infection, particularly in the youngest cases. The study of influenza strains reveals the continuous evolution through either progressive mutations or by segment reassortments. Moreover, the predominant influenza B lineage was different from that included in the recommended vaccine in a half of the studied seasons, supporting the formulation and use of a quadrivalent influenza vaccine. Regarding neuraminidase inhibitors resistance, with the exception of 2007-2008 H275Y seasonal A(H1N1) strains, no other circulating influenza strains carrying known resistance genetic markers were found. Moreover, all circulating A(H1N1)pdm09 and A(H3N2) strains finally became geneticallyresistant to adamantanes. A wide knowledge of the seasonality patterns of the RV in the general population is well-appreciated, but it is a challenge due to the unpredictable RV circulation, highlighting the value of local and global respiratory virus surveillance.
Copyright ? 2016. Published by Elsevier Ltd.
[h=4]KEYWORDS:[/h] influenza viruses; molecular epidemiology; respiratory syncytial viruses; respiratory viruses; surveillance; type/subtype/lineage
PMID: 26939538 [PubMed - as supplied by publisher]
[h=1]Virological surveillance of influenza and other respiratory viruses during six consecutive seasons from 2006 to 2012 in Catalonia, Spain.[/h] Anton A[SUP]1[/SUP], Marcos MA[SUP]2[/SUP], Torner N[SUP]3[/SUP], Isanta R[SUP]2[/SUP], Camps M[SUP]2[/SUP], Martinez A[SUP]3[/SUP], Dom?nguez A[SUP]3[/SUP], Jan? M[SUP]4[/SUP], Jim?nez de Anta MT[SUP]2[/SUP], Pumarola T[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Main attention is given to seasonal influenza and respiratory syncytial viruses outbreaks, but the cumulative burden caused by other respiratory viruses (RV) is not widely considered. The aim of the present study is to describe the RV circulation in the general population during six consecutive seasons from 2006 to 2012 in Catalonia, Spain. Cell culture, immunofluorescence and PCR-based assays were used for the RV laboratory-confirmation and subtyping. Phylogenetic and molecular characterisations of viral HA, NA and M2 were performed from a representative sampling of influenza viruses. A total of 6,315 nasopharyngeal samples were collected, of which the 64% were laboratory-confirmed, mainly by influenza A viruses and rhinoviruses. Results show the significant burden of viral aetiological agents in acute respiratory infection, particularly in the youngest cases. The study of influenza strains reveals the continuous evolution through either progressive mutations or by segment reassortments. Moreover, the predominant influenza B lineage was different from that included in the recommended vaccine in a half of the studied seasons, supporting the formulation and use of a quadrivalent influenza vaccine. Regarding neuraminidase inhibitors resistance, with the exception of 2007-2008 H275Y seasonal A(H1N1) strains, no other circulating influenza strains carrying known resistance genetic markers were found. Moreover, all circulating A(H1N1)pdm09 and A(H3N2) strains finally became geneticallyresistant to adamantanes. A wide knowledge of the seasonality patterns of the RV in the general population is well-appreciated, but it is a challenge due to the unpredictable RV circulation, highlighting the value of local and global respiratory virus surveillance.
Copyright ? 2016. Published by Elsevier Ltd.
[h=4]KEYWORDS:[/h] influenza viruses; molecular epidemiology; respiratory syncytial viruses; respiratory viruses; surveillance; type/subtype/lineage
PMID: 26939538 [PubMed - as supplied by publisher]