tetano
Editor, Senior Moderator
Virol Sin
. 2023 Nov 13:S1995-820X(23)00135-9.
doi: 10.1016/j.virs.2023.11.003. Online ahead of print. Pangolin HKU4-related coronaviruses found in greater bamboo bat from southern China
Min Guo[SUP] 1 [/SUP], Kai Zhao[SUP] 2 [/SUP], Xingwen Peng[SUP] 1 [/SUP], Xiangyang He[SUP] 1 [/SUP], Jin Deng[SUP] 1 [/SUP], Bo Wang[SUP] 3 [/SUP], Xinglou Yang[SUP] 4 [/SUP], Libiao Zhang[SUP] 5 [/SUP]
Affiliations
Coronavirus (CoV) spillover originating from game animals, particularly pangolins, is currently a significant concern. Meanwhile, vigilance is urgently needed for coronaviruses carried by bats, recognised as natural reservoirs of many coronaviruses. In this study, we collected 729 anal swabs of 20 different bat species from nine locations in Yunnan and Guangdong provinces, southern China, in 2016 and 2017 and described the molecular characteristics and genetic diversity of alphacoronaviruses (αCoVs) and betacoronaviruses (βCoVs) in bats. Using RT-PCR, we detected 58 (8.0%) bat CoVs in nine bat species from six locations. Furthermore, using the Illumina platform, we sequenced two representative full-length genomes of the bat CoVs, TyRo-CoV-162275 and TyRo-CoV-162269. Sequence analysis showed that TyRo-CoV-162275 shared the highest identity with Malayan pangolin (Manis javanica) HKU4-related coronaviruses (MjHKU4r-CoVs) from Guangxi Province, whereas TyRo-CoV-162269 was closely related to HKU33-CoV discovered in a greater bamboo bat (Tylonycteris robustula) from Guizhou Province. Notably, TyRo-CoV-162275 has putative furin protease cleavage site in its S protein and likely uses human dipeptidyl peptidase-4 (hDPP4) as a cell-entry receptor, similar to MERS-CoV. To the best of our knowledge, this is the first report of a bat HKU4r-CoV strain with a furin protease cleavage site. These findings expand our understanding of coronavirus geographic and host distributions.
Keywords: Tylonycteris robustula; bat HKU4r-CoV; coronavirus; pangolin HKU4r-CoV; spillover risk.
. 2023 Nov 13:S1995-820X(23)00135-9.
doi: 10.1016/j.virs.2023.11.003. Online ahead of print. Pangolin HKU4-related coronaviruses found in greater bamboo bat from southern China
Min Guo[SUP] 1 [/SUP], Kai Zhao[SUP] 2 [/SUP], Xingwen Peng[SUP] 1 [/SUP], Xiangyang He[SUP] 1 [/SUP], Jin Deng[SUP] 1 [/SUP], Bo Wang[SUP] 3 [/SUP], Xinglou Yang[SUP] 4 [/SUP], Libiao Zhang[SUP] 5 [/SUP]
Affiliations
- PMID: 37967719
- DOI: 10.1016/j.virs.2023.11.003
Coronavirus (CoV) spillover originating from game animals, particularly pangolins, is currently a significant concern. Meanwhile, vigilance is urgently needed for coronaviruses carried by bats, recognised as natural reservoirs of many coronaviruses. In this study, we collected 729 anal swabs of 20 different bat species from nine locations in Yunnan and Guangdong provinces, southern China, in 2016 and 2017 and described the molecular characteristics and genetic diversity of alphacoronaviruses (αCoVs) and betacoronaviruses (βCoVs) in bats. Using RT-PCR, we detected 58 (8.0%) bat CoVs in nine bat species from six locations. Furthermore, using the Illumina platform, we sequenced two representative full-length genomes of the bat CoVs, TyRo-CoV-162275 and TyRo-CoV-162269. Sequence analysis showed that TyRo-CoV-162275 shared the highest identity with Malayan pangolin (Manis javanica) HKU4-related coronaviruses (MjHKU4r-CoVs) from Guangxi Province, whereas TyRo-CoV-162269 was closely related to HKU33-CoV discovered in a greater bamboo bat (Tylonycteris robustula) from Guizhou Province. Notably, TyRo-CoV-162275 has putative furin protease cleavage site in its S protein and likely uses human dipeptidyl peptidase-4 (hDPP4) as a cell-entry receptor, similar to MERS-CoV. To the best of our knowledge, this is the first report of a bat HKU4r-CoV strain with a furin protease cleavage site. These findings expand our understanding of coronavirus geographic and host distributions.
Keywords: Tylonycteris robustula; bat HKU4r-CoV; coronavirus; pangolin HKU4r-CoV; spillover risk.