tetano
Editor, Senior Moderator
Virol J
. 2022 Oct 12;19(1):162.
doi: 10.1186/s12985-022-01888-x.
Transmission and pathogenicity of canine H3N2 influenza virus in dog and guinea pig models
Ratanaporn Tangwangvivat[SUP] 1 2 3 [/SUP], Supassama Chaiyawong[SUP] 1 2 [/SUP], Nutthawan Nonthabenjawan[SUP] 1 2 [/SUP], Kamonpan Charoenkul[SUP] 1 2 [/SUP], Taveesak Janethanakit[SUP] 1 2 [/SUP], Kitikhun Udom[SUP] 1 2 [/SUP], Sawang Kesdangsakonwut[SUP] 4 [/SUP], Rachod Tantilertcharoen[SUP] 4 [/SUP], Aunyaratana Thontiravong[SUP] 1 5 [/SUP], Alongkorn Amonsin[SUP] 6 7 [/SUP]
Affiliations
Abstract
Background: Influenza A virus causes respiratory disease in many animal species as well as in humans. Due to the high human-animal interface, the monitoring of canine influenza in dogs and the study of the transmission and pathogenicity of canine influenza in animals are important.
Methods: Eight-week-old beagle dogs (Canis lupus familaris) (n = 13) were used for the intraspecies transmission model. The dogs were inoculated intranasally with 1 ml of 10[SUP]6[/SUP] EID[SUB]50[/SUB] per ml of canine H3N2 influenza virus (A/canine/Thailand/CU-DC5299/2012) (CIV-H3N2). In addition, 4-week-old guinea pigs (Cavia porcellus) (n = 20) were used for the interspecies transmission model. The guinea pigs were inoculated intranasally with 300 µl of 10[SUP]6[/SUP] EID[SUB]50[/SUB] per ml of CIV-H3N2.
Results: For the Thai CIV-H3N2 challenged in the dog model, the incoculated and direct contact dogs developed respiratory signs at 2 dpi. The dogs shed the virus in the respiratory tract at 1 dpi and developed an H3-specific antibody against the virus at 10 dpi. Lung congestion and histopathological changes in the lung were observed. For the Thai CIV-H3N2 challenge in the guinea pig model, the incoculated, direct contact and aerosol-exposed guinea pigs developed fever at 1-2 dpi. The guinea pigs shed virus in the respiratory tract at 2 dpi and developed an H3-specific antibody against the virus at 7 dpi. Mild histopathological changes in the lung were observed.
Conclusion: The result of this study demonstrated evidence of intraspecies and interspecies transmission of CIV-H3N2 in a mammalian model.
Keywords: Canine influenza; Dog; Guinea pigs; H3N2; Pathogenicity; Transmission.
. 2022 Oct 12;19(1):162.
doi: 10.1186/s12985-022-01888-x.
Transmission and pathogenicity of canine H3N2 influenza virus in dog and guinea pig models
Ratanaporn Tangwangvivat[SUP] 1 2 3 [/SUP], Supassama Chaiyawong[SUP] 1 2 [/SUP], Nutthawan Nonthabenjawan[SUP] 1 2 [/SUP], Kamonpan Charoenkul[SUP] 1 2 [/SUP], Taveesak Janethanakit[SUP] 1 2 [/SUP], Kitikhun Udom[SUP] 1 2 [/SUP], Sawang Kesdangsakonwut[SUP] 4 [/SUP], Rachod Tantilertcharoen[SUP] 4 [/SUP], Aunyaratana Thontiravong[SUP] 1 5 [/SUP], Alongkorn Amonsin[SUP] 6 7 [/SUP]
Affiliations
- PMID: 36224594
- DOI: 10.1186/s12985-022-01888-x
Abstract
Background: Influenza A virus causes respiratory disease in many animal species as well as in humans. Due to the high human-animal interface, the monitoring of canine influenza in dogs and the study of the transmission and pathogenicity of canine influenza in animals are important.
Methods: Eight-week-old beagle dogs (Canis lupus familaris) (n = 13) were used for the intraspecies transmission model. The dogs were inoculated intranasally with 1 ml of 10[SUP]6[/SUP] EID[SUB]50[/SUB] per ml of canine H3N2 influenza virus (A/canine/Thailand/CU-DC5299/2012) (CIV-H3N2). In addition, 4-week-old guinea pigs (Cavia porcellus) (n = 20) were used for the interspecies transmission model. The guinea pigs were inoculated intranasally with 300 µl of 10[SUP]6[/SUP] EID[SUB]50[/SUB] per ml of CIV-H3N2.
Results: For the Thai CIV-H3N2 challenged in the dog model, the incoculated and direct contact dogs developed respiratory signs at 2 dpi. The dogs shed the virus in the respiratory tract at 1 dpi and developed an H3-specific antibody against the virus at 10 dpi. Lung congestion and histopathological changes in the lung were observed. For the Thai CIV-H3N2 challenge in the guinea pig model, the incoculated, direct contact and aerosol-exposed guinea pigs developed fever at 1-2 dpi. The guinea pigs shed virus in the respiratory tract at 2 dpi and developed an H3-specific antibody against the virus at 7 dpi. Mild histopathological changes in the lung were observed.
Conclusion: The result of this study demonstrated evidence of intraspecies and interspecies transmission of CIV-H3N2 in a mammalian model.
Keywords: Canine influenza; Dog; Guinea pigs; H3N2; Pathogenicity; Transmission.