tetano
Editor, Senior Moderator
Virol J
. 2023 Apr 24;20(1):78.
doi: 10.1186/s12985-023-02027-w.
Independent role of caspases and Bik in augmenting influenza A virus replication in airway epithelial cells and mice
Sourabh Soni[SUP] 1 [/SUP], Stephanie Walton-Filipczak[SUP] 2 3 [/SUP], Richard S Nho[SUP] 1 [/SUP], Yohannes Tesfaigzi[SUP] 4 [/SUP], Yohannes A Mebratu[SUP] 5 [/SUP]
Affiliations
Abstract
Caspases and poly (ADP-ribose) polymerase 1 (PARP1) have been shown to promote influenza A virus (IAV) replication. However, the relative importance and molecular mechanisms of specific caspases and their downstream substrate PARP1 in regulating viral replication in airway epithelial cells (AECs) remains incompletely elucidated. Here, we targeted caspase 2, 3, 6, and PARP1 using specific inhibitors to compare their role in promoting IAV replication. Inhibition of each of these proteins caused significant decline in viral titer, although PARP1 inhibitor led to the most robust reduction of viral replication. We previously showed that the pro-apoptotic protein Bcl-2 interacting killer (Bik) promotes IAV replication in the AECs by activating caspase 3. In this study, we found that as compared with AECs from wild-type mice, bik-deficiency alone resulted in ~ 3 logs reduction in virus titer in the absence of treatment with the pan-caspase inhibitor (Q-VD-Oph). Inhibiting overall caspase activity using Q-VD-Oph caused additional decline in viral titer by ~ 1 log in bik[SUP]-/-[/SUP] AECs. Similarly, mice treated with Q-VD-Oph were protected from IAV-induced lung inflammation and lethality. Inhibiting caspase activity diminished nucleo-cytoplasmic transport of viral nucleoprotein (NP) and cleavage of viral hemagglutinin and NP in human AECs. These findings suggest that caspases and PARP1 play major roles to independently promote IAV replication and that additional mechanism(s) independent of caspases and PARP1 may be involved in Bik-mediated IAV replication. Further, peptides or inhibitors that target and block multiple caspases or PARP1 may be effective treatment targets for influenza infection.
Keywords: Bik wild-type and knockout mice; Caspase; Influenza A virus; PARP1; Q-VD-Oph.
. 2023 Apr 24;20(1):78.
doi: 10.1186/s12985-023-02027-w.
Independent role of caspases and Bik in augmenting influenza A virus replication in airway epithelial cells and mice
Sourabh Soni[SUP] 1 [/SUP], Stephanie Walton-Filipczak[SUP] 2 3 [/SUP], Richard S Nho[SUP] 1 [/SUP], Yohannes Tesfaigzi[SUP] 4 [/SUP], Yohannes A Mebratu[SUP] 5 [/SUP]
Affiliations
- PMID: 37095508
- DOI: 10.1186/s12985-023-02027-w
Abstract
Caspases and poly (ADP-ribose) polymerase 1 (PARP1) have been shown to promote influenza A virus (IAV) replication. However, the relative importance and molecular mechanisms of specific caspases and their downstream substrate PARP1 in regulating viral replication in airway epithelial cells (AECs) remains incompletely elucidated. Here, we targeted caspase 2, 3, 6, and PARP1 using specific inhibitors to compare their role in promoting IAV replication. Inhibition of each of these proteins caused significant decline in viral titer, although PARP1 inhibitor led to the most robust reduction of viral replication. We previously showed that the pro-apoptotic protein Bcl-2 interacting killer (Bik) promotes IAV replication in the AECs by activating caspase 3. In this study, we found that as compared with AECs from wild-type mice, bik-deficiency alone resulted in ~ 3 logs reduction in virus titer in the absence of treatment with the pan-caspase inhibitor (Q-VD-Oph). Inhibiting overall caspase activity using Q-VD-Oph caused additional decline in viral titer by ~ 1 log in bik[SUP]-/-[/SUP] AECs. Similarly, mice treated with Q-VD-Oph were protected from IAV-induced lung inflammation and lethality. Inhibiting caspase activity diminished nucleo-cytoplasmic transport of viral nucleoprotein (NP) and cleavage of viral hemagglutinin and NP in human AECs. These findings suggest that caspases and PARP1 play major roles to independently promote IAV replication and that additional mechanism(s) independent of caspases and PARP1 may be involved in Bik-mediated IAV replication. Further, peptides or inhibitors that target and block multiple caspases or PARP1 may be effective treatment targets for influenza infection.
Keywords: Bik wild-type and knockout mice; Caspase; Influenza A virus; PARP1; Q-VD-Oph.