tetano
Editor, Senior Moderator
Virol J
. 2021 Dec 7;18(1):244.
doi: 10.1186/s12985-021-01717-7.
Expression of plasma IFN signaling-related miRNAs during acute SARS-CoV-2 infection and its association with RBD-IgG antibody response
Jing Wu[SUP] #[/SUP][SUP] 1 [/SUP], Xingxiang Liu[SUP] #[/SUP][SUP] 2 [/SUP], Jianguo Shao[SUP] #[/SUP][SUP] 3 [/SUP], Yuanyuan Zhang[SUP] 2 [/SUP], Renfei Lu[SUP] 3 [/SUP], Hong Xue[SUP] 3 [/SUP], Yunfang Xu[SUP] 2 [/SUP], Lijuan Wang[SUP] 2 [/SUP], Hui Zhou[SUP] 4 [/SUP], Lugang Yu[SUP] 4 [/SUP], Ming Yue[SUP] 5 [/SUP], Chen Dong[SUP] 6 [/SUP]
Affiliations
Abstract
Background: Coronavirus disease 2019 (COVID-19) is a huge challenge worldwide. Although previous studies have suggested that type I interferon (IFN-I) could inhibit the virus replication, the expression characteristics of IFN-I signaling-related miRNAs (ISR-miRNAs) during acute severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection and its relationship with receptor-binding domain (RBD) IgG antibody response at the recovery phase remain unclear.
Methods: Expression profiles of 12 plasma ISR-miRNAs in COVID-19 patients and healthy controls were analyzed using RT-qPCR. The level of RBD-IgG antibody was determined using the competitive ELISA. Spearman correlation was done to measure the associations of plasma ISR-miRNAs with clinical characteristics during acute SARS-CoV-2 infection and RBD-IgG antibody response at the recovery phase.
Results: Compared with the healthy controls, COVID-19 patients exhibited higher levels of miR-29b-3p (Z = 3.15, P = 0.002) and miR-1246 (Z = 4.98, P < 0.001). However, the expression of miR-186-5p and miR-15a-5p were significantly decreased. As the results shown, miR-30b-5p was negatively correlated with CD4 + T cell counts (r = - 0.41, P = 0.027) and marginally positively correlated with fasting plasma glucose in COVID-19 patients (r = 0.37, P = 0.052). The competitive ELISA analysis showed the plasma level of miR-497-5p at the acute phase was positively correlated with RBD-IgG antibody response (r = 0.48, P = 0.038).
Conclusions: Our present results suggested that the expression level of ISR-miRNAs was not only associated with acute SARS-CoV-2 infection but also with RBD-IgG antibody response at the recovery phase of COVID-19. Future studies should be performed to explore the biological significance of ISR-miRNAs in SARS-CoV-2 infection.
Keywords: COVID-19; IFN-I signaling pathway; MicroRNA; RBD-IgG; SARS-CoV-2.
. 2021 Dec 7;18(1):244.
doi: 10.1186/s12985-021-01717-7.
Expression of plasma IFN signaling-related miRNAs during acute SARS-CoV-2 infection and its association with RBD-IgG antibody response
Jing Wu[SUP] #[/SUP][SUP] 1 [/SUP], Xingxiang Liu[SUP] #[/SUP][SUP] 2 [/SUP], Jianguo Shao[SUP] #[/SUP][SUP] 3 [/SUP], Yuanyuan Zhang[SUP] 2 [/SUP], Renfei Lu[SUP] 3 [/SUP], Hong Xue[SUP] 3 [/SUP], Yunfang Xu[SUP] 2 [/SUP], Lijuan Wang[SUP] 2 [/SUP], Hui Zhou[SUP] 4 [/SUP], Lugang Yu[SUP] 4 [/SUP], Ming Yue[SUP] 5 [/SUP], Chen Dong[SUP] 6 [/SUP]
Affiliations
- PMID: 34876159
- DOI: 10.1186/s12985-021-01717-7
Abstract
Background: Coronavirus disease 2019 (COVID-19) is a huge challenge worldwide. Although previous studies have suggested that type I interferon (IFN-I) could inhibit the virus replication, the expression characteristics of IFN-I signaling-related miRNAs (ISR-miRNAs) during acute severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection and its relationship with receptor-binding domain (RBD) IgG antibody response at the recovery phase remain unclear.
Methods: Expression profiles of 12 plasma ISR-miRNAs in COVID-19 patients and healthy controls were analyzed using RT-qPCR. The level of RBD-IgG antibody was determined using the competitive ELISA. Spearman correlation was done to measure the associations of plasma ISR-miRNAs with clinical characteristics during acute SARS-CoV-2 infection and RBD-IgG antibody response at the recovery phase.
Results: Compared with the healthy controls, COVID-19 patients exhibited higher levels of miR-29b-3p (Z = 3.15, P = 0.002) and miR-1246 (Z = 4.98, P < 0.001). However, the expression of miR-186-5p and miR-15a-5p were significantly decreased. As the results shown, miR-30b-5p was negatively correlated with CD4 + T cell counts (r = - 0.41, P = 0.027) and marginally positively correlated with fasting plasma glucose in COVID-19 patients (r = 0.37, P = 0.052). The competitive ELISA analysis showed the plasma level of miR-497-5p at the acute phase was positively correlated with RBD-IgG antibody response (r = 0.48, P = 0.038).
Conclusions: Our present results suggested that the expression level of ISR-miRNAs was not only associated with acute SARS-CoV-2 infection but also with RBD-IgG antibody response at the recovery phase of COVID-19. Future studies should be performed to explore the biological significance of ISR-miRNAs in SARS-CoV-2 infection.
Keywords: COVID-19; IFN-I signaling pathway; MicroRNA; RBD-IgG; SARS-CoV-2.