tetano
Editor, Senior Moderator
Virol J
. 2025 Jul 5;22(1):223.
doi: 10.1186/s12985-025-02793-9. Dynamic adaptation mutations and pathogenic characterization of a mouse-adapted seasonal human H3N2 influenza virus
Cheng Zhang[SUP] 1 2 [/SUP], Yan Li[SUP] 2 [/SUP], Ning Zhang[SUP] 2 [/SUP], Ju Sun[SUP] 2 [/SUP], Deyu Tian[SUP] 2 [/SUP], Xuefeng Duan[SUP] 2 3 [/SUP], Jing Yang[SUP] 4 [/SUP], Yuhai Bi[SUP] 5 6 7 [/SUP]
Affiliations
Background: H3N2 influenza A viruses [A(H3N2)] circulate as seasonal influenza in humans worldwide, resulting in a huge disease burden. Adaptation study of A(H3N2) in mice could provide a basis for preclinical evaluation of antivirals and vaccines targeting A(H3N2) and identify the genetic markers responsible for the viral adaptation, replication, and pathogenesis.
Methods: Lung-to-lung passaging of wild-type (WT) A(H3N2) strain was performed in C57BL/6J mice. Amino acid (AA) mutations occurred during the passaging and temporal dynamics of these mutations were identified using the next-generation sequencing. We determined the polymerase activity of the ribonucleoprotein (RNP) complex containing mutation genes and compared the pathogenicity between the mouse-adapted (MA) and A(H3N2)-WT strains based on body weight change, survival rate, lung index, lung viral load, and lung pathology of the infected mice.
Results: The A(H3N2)-MA strain was obtained after seventeen lung-to-lung passages in mice. 14 AA mutations in the PB2, PB1, PA, HA, NP, and M1 genes were identified in the A(H3N2)-MA strain compared to the A(H3N2)-WT strain. In addition, the polymerase activity of the RNP complex containing mutation genes was increased, and the pathogenicity of the MA virus is significantly higher than that of the WT strain.
Conclusions: One A(H3N2)-MA strain has been developed that can infect and kill mice. The MA strain showed stronger replication ability and pathogenicity than the A(H3N2)-WT strain. This A(H3N2)-MA model provides a valuable basis for evaluating the effects of drugs and vaccines and for studying pathogenesis.
Keywords: Genetic markers; H3N2; Influenza A virus; Mouse adaptation; Mouse model; Mutations; Pathogenicity.
. 2025 Jul 5;22(1):223.
doi: 10.1186/s12985-025-02793-9. Dynamic adaptation mutations and pathogenic characterization of a mouse-adapted seasonal human H3N2 influenza virus
Cheng Zhang[SUP] 1 2 [/SUP], Yan Li[SUP] 2 [/SUP], Ning Zhang[SUP] 2 [/SUP], Ju Sun[SUP] 2 [/SUP], Deyu Tian[SUP] 2 [/SUP], Xuefeng Duan[SUP] 2 3 [/SUP], Jing Yang[SUP] 4 [/SUP], Yuhai Bi[SUP] 5 6 7 [/SUP]
Affiliations
- PMID: 40618079
- PMCID: PMC12229041
- DOI: 10.1186/s12985-025-02793-9
Background: H3N2 influenza A viruses [A(H3N2)] circulate as seasonal influenza in humans worldwide, resulting in a huge disease burden. Adaptation study of A(H3N2) in mice could provide a basis for preclinical evaluation of antivirals and vaccines targeting A(H3N2) and identify the genetic markers responsible for the viral adaptation, replication, and pathogenesis.
Methods: Lung-to-lung passaging of wild-type (WT) A(H3N2) strain was performed in C57BL/6J mice. Amino acid (AA) mutations occurred during the passaging and temporal dynamics of these mutations were identified using the next-generation sequencing. We determined the polymerase activity of the ribonucleoprotein (RNP) complex containing mutation genes and compared the pathogenicity between the mouse-adapted (MA) and A(H3N2)-WT strains based on body weight change, survival rate, lung index, lung viral load, and lung pathology of the infected mice.
Results: The A(H3N2)-MA strain was obtained after seventeen lung-to-lung passages in mice. 14 AA mutations in the PB2, PB1, PA, HA, NP, and M1 genes were identified in the A(H3N2)-MA strain compared to the A(H3N2)-WT strain. In addition, the polymerase activity of the RNP complex containing mutation genes was increased, and the pathogenicity of the MA virus is significantly higher than that of the WT strain.
Conclusions: One A(H3N2)-MA strain has been developed that can infect and kill mice. The MA strain showed stronger replication ability and pathogenicity than the A(H3N2)-WT strain. This A(H3N2)-MA model provides a valuable basis for evaluating the effects of drugs and vaccines and for studying pathogenesis.
Keywords: Genetic markers; H3N2; Influenza A virus; Mouse adaptation; Mouse model; Mutations; Pathogenicity.