Giuseppe
Emeritus
Course of seasonal influenza A/Brisbane/59/07 H1N1 infection in the ferret (Virol J., abstract, edited)
[Source: BMC/Virology Journal, <cite cite="http://www.virologyj.com/content/7/1/149/abstract">Abstract | Course of seasonal influenza A/Brisbane/59/07 H1N1 infection in the ferret</cite>. Abstract, edited.]
Course of seasonal influenza A/Brisbane/59/07 H1N1 infection in the ferret
Alexis McBrayer, Jeremy V Camp, Ronald Tapp, Vladimir Yamshchikov, Shiela Grimes, Diana L Noah, Colleen B Jonsson and Carl E Bruder
Virology Journal 2010, 7:149
doi:10.1186/1743-422X-7-149
Published: 9 July 2010
Abstract (provisional)
Every year, influenza viruses infect approximately 5-20% of the population in the United States leading to over 200,000 hospitalizations and 36,000 deaths from flu-related complications. In this study, we characterized the immune and pathological progression of a seasonal strain of H1N1 influenza virus, A/Brisbane/59/2007. The immune response of the ferrets showed a dose-dependent increase with increased virus challenge, as indicated by the presence of virus specific IgG, IgM, and neutralizing antibodies. Animals infected with higher doses of virus also experienced increasing severity of clinical symptoms and fever at 2 days post-infection (DPI). Interestingly, weight loss was more pronounced in animals infected with lower doses of virus compared to those infected with a higher dose; these results were consistent with viral titers of swabs collected from the nares, but not the throat. Analyzed specimens included nasal and throat swabs from 1, 3, 5, and 7 DPI as well as tissue samples from caudal lung and nasal turbinates. Viral titers of the swab samples in all groups were higher on 1 and 3 DPI and returned to baseline levels by 7 DPI. Analysis of nasal turbinates indicates presence of virus at 3 DPI in all infected groups, whereas virus was only detected in the lungs of animals in the two highest dose groups. Histological analysis of the lungs showed a range of pathology, such as chronic inflammation and bronchial epithelial hypertrophy. The results provided here offer important endpoints for preclinical testing of the efficacy of new antiviral compounds and experimental vaccines.
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[Source: BMC/Virology Journal, <cite cite="http://www.virologyj.com/content/7/1/149/abstract">Abstract | Course of seasonal influenza A/Brisbane/59/07 H1N1 infection in the ferret</cite>. Abstract, edited.]
Course of seasonal influenza A/Brisbane/59/07 H1N1 infection in the ferret
Alexis McBrayer, Jeremy V Camp, Ronald Tapp, Vladimir Yamshchikov, Shiela Grimes, Diana L Noah, Colleen B Jonsson and Carl E Bruder
Virology Journal 2010, 7:149
doi:10.1186/1743-422X-7-149
Published: 9 July 2010
Abstract (provisional)
Every year, influenza viruses infect approximately 5-20% of the population in the United States leading to over 200,000 hospitalizations and 36,000 deaths from flu-related complications. In this study, we characterized the immune and pathological progression of a seasonal strain of H1N1 influenza virus, A/Brisbane/59/2007. The immune response of the ferrets showed a dose-dependent increase with increased virus challenge, as indicated by the presence of virus specific IgG, IgM, and neutralizing antibodies. Animals infected with higher doses of virus also experienced increasing severity of clinical symptoms and fever at 2 days post-infection (DPI). Interestingly, weight loss was more pronounced in animals infected with lower doses of virus compared to those infected with a higher dose; these results were consistent with viral titers of swabs collected from the nares, but not the throat. Analyzed specimens included nasal and throat swabs from 1, 3, 5, and 7 DPI as well as tissue samples from caudal lung and nasal turbinates. Viral titers of the swab samples in all groups were higher on 1 and 3 DPI and returned to baseline levels by 7 DPI. Analysis of nasal turbinates indicates presence of virus at 3 DPI in all infected groups, whereas virus was only detected in the lungs of animals in the two highest dose groups. Histological analysis of the lungs showed a range of pathology, such as chronic inflammation and bronchial epithelial hypertrophy. The results provided here offer important endpoints for preclinical testing of the efficacy of new antiviral compounds and experimental vaccines.
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