tetano
Editor, Senior Moderator
Virol J
. 2021 Aug 19;18(1):171.
doi: 10.1186/s12985-021-01641-w.
Antiviral activity of diallyl trisulfide against H9N2 avian influenza virus infection in vitro and in vivo
Le Ming[SUP] 1 [/SUP], Zhihui Li[SUP] 1 [/SUP], Xiaofang Li[SUP] 1 [/SUP], Ling Tang[SUP] 1 [/SUP], Guimei He[SUP] 2 3 [/SUP]
Affiliations
Abstract
Background: Diallyl trisulfide (DATS) is a garlic-derived organosulfur compound. As it has been shown to have anti-viral activity, we hypothesized that it may alleviate infections caused by H9N2 avian influenza virus (AIV), which is prevalent in poultry with pandemic potential.
Methods: Human lung A549 epithelial cells were treated with three different concentrations of DATS 24 h before (pre-treatment) or one hour after (post-treatment) H9N2 AIV infection. Culture supernatants were collected 24 h and 48 h post-infection and analyzed for viral titers and levels of inflammatory and anti-viral immune responses. For in vivo experiments, BABL/c mice were administered daily by intraperitoneal injection with DATS (30 mg/kg) for 2 weeks starting 1 day after H9N2 AIV infection. Clinical signs, lung pathology, and inflammatory and anti-viral immune responses were assessed 2, 4, and 6 days after infection.
Results: Both pre-treatment and post-treatment of A549 cells with DATS resulted in reduced viral loads, increased expression of anti-viral genes (RIG-I, IRF-3, and interferon-β), and decreased expression of inflammatory cytokines (TNF-α and IL-6). These effects were also observed in H9N2 AIV-infected mice treated with DATS. Such treatment also reduced lung edema and inflammation in mice.
Conclusions: Results suggest that DATS has anti-viral activity against H9N2 AIV and may be used as an alternative treatment for influenza virus infection.
Keywords: Antiviral activity; Diallyl trisulfide; H9N2 AIV; Inflammation.
. 2021 Aug 19;18(1):171.
doi: 10.1186/s12985-021-01641-w.
Antiviral activity of diallyl trisulfide against H9N2 avian influenza virus infection in vitro and in vivo
Le Ming[SUP] 1 [/SUP], Zhihui Li[SUP] 1 [/SUP], Xiaofang Li[SUP] 1 [/SUP], Ling Tang[SUP] 1 [/SUP], Guimei He[SUP] 2 3 [/SUP]
Affiliations
- PMID: 34412671
- DOI: 10.1186/s12985-021-01641-w
Abstract
Background: Diallyl trisulfide (DATS) is a garlic-derived organosulfur compound. As it has been shown to have anti-viral activity, we hypothesized that it may alleviate infections caused by H9N2 avian influenza virus (AIV), which is prevalent in poultry with pandemic potential.
Methods: Human lung A549 epithelial cells were treated with three different concentrations of DATS 24 h before (pre-treatment) or one hour after (post-treatment) H9N2 AIV infection. Culture supernatants were collected 24 h and 48 h post-infection and analyzed for viral titers and levels of inflammatory and anti-viral immune responses. For in vivo experiments, BABL/c mice were administered daily by intraperitoneal injection with DATS (30 mg/kg) for 2 weeks starting 1 day after H9N2 AIV infection. Clinical signs, lung pathology, and inflammatory and anti-viral immune responses were assessed 2, 4, and 6 days after infection.
Results: Both pre-treatment and post-treatment of A549 cells with DATS resulted in reduced viral loads, increased expression of anti-viral genes (RIG-I, IRF-3, and interferon-β), and decreased expression of inflammatory cytokines (TNF-α and IL-6). These effects were also observed in H9N2 AIV-infected mice treated with DATS. Such treatment also reduced lung edema and inflammation in mice.
Conclusions: Results suggest that DATS has anti-viral activity against H9N2 AIV and may be used as an alternative treatment for influenza virus infection.
Keywords: Antiviral activity; Diallyl trisulfide; H9N2 AIV; Inflammation.